Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/52298 |
Resumo: | Supplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs. |
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Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterialsmelanomagold nanoparticlesbioproductionlaser photothermal therapyin vitro modelsexperimental resultsSupplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs.Cancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompatible gold nanoparticles (GNPs) coated with hyaluronic acid and oleic acid were prepared and characterized in terms of size, morphology and cytotoxicity in the presence of Saccharomyces cerevisiae, and two cell lines, the keratinocytes (healthy skin cells, HaCat) and the melanoma cells (B16F10). Results showed that these GNPs absorb within the near-infrared region (750–1400 nm), in the optical therapeutic window (from 650 to 1300 nm), in contrast to other commercial gold nanoparticles, which enables light to penetrate into deep skin layers. A laser emitting in this region was applied and its effect also analyzed. The coated GNPs showed a spherical morphology with a mean size of 297 nm without cytotoxic effects towards yeast and tested cell lines. Nevertheless, after laser irradiation, a reduction of 20% in B16F10 cell line viability was observed. In summary, this work appears to be a promising strategy for the treatment of non-metastatic melanoma or other superficial tumors.This research was funded by Fundação para a Ciência e a Tecnologia (FCT) through the projects: UIDB/00645/2020, UID/DTP/04138/2019, PTDC/MED-QUI/31721/2017 as well as by the PhD fellowship SFRH/BD/148044/2019 (J.L.).Repositório da Universidade de LisboaLopes, JoanaCoelho, João Miguel PintoVieira, Pedro Manuel CardosoViana, Ana SilveiraGaspar, Maria ManuelaReis, Catarina Pinto2022-04-12T12:33:24Z2020-08-052022-02-22T10:53:55Z2020-08-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/52298engLopes J, Coelho JMP, Vieira PMC, Viana AS, Gaspar MM, Reis C. Preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials. Nanomaterials [Internet]. 2020;10(8):1536. Disponível em: https://www.mdpi.com/2079-4991/10/8/15362079-4991cv-prod-2446145https://doi.org/10.3390/nano10081536info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:08Zoai:repositorio.ul.pt:10451/52298Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:44.186324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
title |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
spellingShingle |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials Lopes, Joana melanoma gold nanoparticles bioproduction laser photothermal therapy in vitro models experimental results |
title_short |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
title_full |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
title_fullStr |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
title_full_unstemmed |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
title_sort |
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials |
author |
Lopes, Joana |
author_facet |
Lopes, Joana Coelho, João Miguel Pinto Vieira, Pedro Manuel Cardoso Viana, Ana Silveira Gaspar, Maria Manuela Reis, Catarina Pinto |
author_role |
author |
author2 |
Coelho, João Miguel Pinto Vieira, Pedro Manuel Cardoso Viana, Ana Silveira Gaspar, Maria Manuela Reis, Catarina Pinto |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Lopes, Joana Coelho, João Miguel Pinto Vieira, Pedro Manuel Cardoso Viana, Ana Silveira Gaspar, Maria Manuela Reis, Catarina Pinto |
dc.subject.por.fl_str_mv |
melanoma gold nanoparticles bioproduction laser photothermal therapy in vitro models experimental results |
topic |
melanoma gold nanoparticles bioproduction laser photothermal therapy in vitro models experimental results |
description |
Supplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-05 2020-08-05T00:00:00Z 2022-04-12T12:33:24Z 2022-02-22T10:53:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/52298 |
url |
http://hdl.handle.net/10451/52298 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lopes J, Coelho JMP, Vieira PMC, Viana AS, Gaspar MM, Reis C. Preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials. Nanomaterials [Internet]. 2020;10(8):1536. Disponível em: https://www.mdpi.com/2079-4991/10/8/1536 2079-4991 cv-prod-2446145 https://doi.org/10.3390/nano10081536 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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