Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/13597 |
Resumo: | Bare-root transplants of strawberry (Fragaria × ananassa Duch. cv ‘Diamond’) were grown in a Hoagland’s nutrient solution with or without Fe. Forty two days after Fe deprivation, recovery was induced by addition of 10 M of Fe (Fe-EDDHA) to the Fe(−) nutrient solution. Total leaf chlorophyll concentration in young leaves decreased progressively with time in Fe-deprived plants, and before Fe resupply it was only 6% of that of Fe(+) control plants. Spatio-temporal changes on photosynthetic efficiency were monitored by imaging chlorophyll a fluorescence in four areas of interest (AOIs) located on the midrib and on interveinal mesophyll areas of leaf blades. Chlorophyll fluorescence images (Fv/Fm, II, NPQ, qp) showed a large spatial variation, particularly at day 42, with greater values in midrib areas. Temporal changes were also observed in all measured parameters along the experimental period, but the onset and intensity of impact was clearly different between parameters. Maximal efficiency of PSII (Fv/Fm) was the last parameter to be affected, being the effects visible only in plants that had lost over 90% of their chlorophyll (day 42). In contrast, actual efficiency of PSII ( II) and photochemical quenching (qp) were affected earlier on, showing noticeable changes by day 20, when chlorophyll concentration had declined by 38%. Decreases in II were balanced by increases in quantum yield of non-regulated energy dissipation ( NO). Fluorescence parameters, with the exception of II and Fe content, recovered within eight days following Fe resupply to values found in Fe(+) plants. The results of this study indicate that: (i) Chl fluorescence imaging is a useful technique to evaluate Fe deficiency (ii) Fe stress generates spatio-temporal heterogeneity in fluorescence response (iii) II measured in interveinal mesophyll areas could be used as an early and fast indicator of Fe deficiency and could be applied for fertilization management. |
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Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plantschlorophyll fluorescence imagingenergy partitioningfragaria x ananassairon deficiencyphotosynthetic efficiencyBare-root transplants of strawberry (Fragaria × ananassa Duch. cv ‘Diamond’) were grown in a Hoagland’s nutrient solution with or without Fe. Forty two days after Fe deprivation, recovery was induced by addition of 10 M of Fe (Fe-EDDHA) to the Fe(−) nutrient solution. Total leaf chlorophyll concentration in young leaves decreased progressively with time in Fe-deprived plants, and before Fe resupply it was only 6% of that of Fe(+) control plants. Spatio-temporal changes on photosynthetic efficiency were monitored by imaging chlorophyll a fluorescence in four areas of interest (AOIs) located on the midrib and on interveinal mesophyll areas of leaf blades. Chlorophyll fluorescence images (Fv/Fm, II, NPQ, qp) showed a large spatial variation, particularly at day 42, with greater values in midrib areas. Temporal changes were also observed in all measured parameters along the experimental period, but the onset and intensity of impact was clearly different between parameters. Maximal efficiency of PSII (Fv/Fm) was the last parameter to be affected, being the effects visible only in plants that had lost over 90% of their chlorophyll (day 42). In contrast, actual efficiency of PSII ( II) and photochemical quenching (qp) were affected earlier on, showing noticeable changes by day 20, when chlorophyll concentration had declined by 38%. Decreases in II were balanced by increases in quantum yield of non-regulated energy dissipation ( NO). Fluorescence parameters, with the exception of II and Fe content, recovered within eight days following Fe resupply to values found in Fe(+) plants. The results of this study indicate that: (i) Chl fluorescence imaging is a useful technique to evaluate Fe deficiency (ii) Fe stress generates spatio-temporal heterogeneity in fluorescence response (iii) II measured in interveinal mesophyll areas could be used as an early and fast indicator of Fe deficiency and could be applied for fertilization management.ElsevierRepositório da Universidade de LisboaOsório, JúlioOsório, Maria LeonorCorreia, Pedro JoséVarennes, AmarilisPestana, Maribela2017-05-08T09:09:11Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/13597eng"Scientia Horticulturae". ISSN 0304-4238. 165 (2014) p.148-155http://dx.doi.org/10.1016/j.scientia.2013.10.042info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:43:41Zoai:www.repository.utl.pt:10400.5/13597Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:59:33.938408Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
title |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
spellingShingle |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants Osório, Júlio chlorophyll fluorescence imaging energy partitioning fragaria x ananassa iron deficiency photosynthetic efficiency |
title_short |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
title_full |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
title_fullStr |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
title_full_unstemmed |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
title_sort |
Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and ressupply on photosynthetic performance of strawberry plants |
author |
Osório, Júlio |
author_facet |
Osório, Júlio Osório, Maria Leonor Correia, Pedro José Varennes, Amarilis Pestana, Maribela |
author_role |
author |
author2 |
Osório, Maria Leonor Correia, Pedro José Varennes, Amarilis Pestana, Maribela |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Osório, Júlio Osório, Maria Leonor Correia, Pedro José Varennes, Amarilis Pestana, Maribela |
dc.subject.por.fl_str_mv |
chlorophyll fluorescence imaging energy partitioning fragaria x ananassa iron deficiency photosynthetic efficiency |
topic |
chlorophyll fluorescence imaging energy partitioning fragaria x ananassa iron deficiency photosynthetic efficiency |
description |
Bare-root transplants of strawberry (Fragaria × ananassa Duch. cv ‘Diamond’) were grown in a Hoagland’s nutrient solution with or without Fe. Forty two days after Fe deprivation, recovery was induced by addition of 10 M of Fe (Fe-EDDHA) to the Fe(−) nutrient solution. Total leaf chlorophyll concentration in young leaves decreased progressively with time in Fe-deprived plants, and before Fe resupply it was only 6% of that of Fe(+) control plants. Spatio-temporal changes on photosynthetic efficiency were monitored by imaging chlorophyll a fluorescence in four areas of interest (AOIs) located on the midrib and on interveinal mesophyll areas of leaf blades. Chlorophyll fluorescence images (Fv/Fm, II, NPQ, qp) showed a large spatial variation, particularly at day 42, with greater values in midrib areas. Temporal changes were also observed in all measured parameters along the experimental period, but the onset and intensity of impact was clearly different between parameters. Maximal efficiency of PSII (Fv/Fm) was the last parameter to be affected, being the effects visible only in plants that had lost over 90% of their chlorophyll (day 42). In contrast, actual efficiency of PSII ( II) and photochemical quenching (qp) were affected earlier on, showing noticeable changes by day 20, when chlorophyll concentration had declined by 38%. Decreases in II were balanced by increases in quantum yield of non-regulated energy dissipation ( NO). Fluorescence parameters, with the exception of II and Fe content, recovered within eight days following Fe resupply to values found in Fe(+) plants. The results of this study indicate that: (i) Chl fluorescence imaging is a useful technique to evaluate Fe deficiency (ii) Fe stress generates spatio-temporal heterogeneity in fluorescence response (iii) II measured in interveinal mesophyll areas could be used as an early and fast indicator of Fe deficiency and could be applied for fertilization management. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z 2017-05-08T09:09:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/13597 |
url |
http://hdl.handle.net/10400.5/13597 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Scientia Horticulturae". ISSN 0304-4238. 165 (2014) p.148-155 http://dx.doi.org/10.1016/j.scientia.2013.10.042 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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