Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/95690 https://doi.org/10.1186/s12865-021-00423-x |
Resumo: | Background Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveries. The ability of innate lymphoid cells (ILCs) to act as pro-inflammatory mediators arose the interest to study their role in normal and pathological pregnancies. The aim of this work was to analyze the relative frequencies of ILCs subsets in pregnancy and the levels of IL-4, IL-17, IL-22, and IFN-γ as inflammatory mediators. Accordingly, we hypothesized that changes in the proportions of ILCs subpopulations could be related to preterm birth. Methods We analyzed 15 full-term delivery samples and six preterm delivery samples. In the full-term group (FTB) peripheral blood was taken during routine blood analysis, on 3 occasions: 1st, 2nd and 3rd trimester. After delivery, peripheral blood, cord blood and placenta were collected. In PTB group, peripheral blood samples were obtained on two occasions: before and 24 h after treatment with progesterone. We used flow cytometry to analyze ILCs in maternal peripheral blood, placenta, and cord blood samples. Maternal peripheral blood and cord blood samples were analyzed by enzyme-linked immunosorbent assay for IL-4, IL-17, IL-22, and IFN-γ plasma levels at the time of labor. Results We observed significantly increased relative frequencies of ILC2 and ILC3 in the decidua, as well as an increase of ILC2 in cord blood samples in PTB group, compared to FTB samples. We also found a decrease in IFN-γ in peripheral blood samples of the PTB group, suggesting a functional withdrawal. Additionally, IL-4, IL-17, IL-22 levels were similar in PTB and FTB groups, denoting a relevant role in mediating labor. Conclusion Our results suggest that ILC2 and ILC3 play a role in PTB by mediating an inflammatory response. Further work is necessary to evaluate the importance of ILCs in the regulation of labor. |
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Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birthPreterm birthInflammationInnate immune responseInnate lymphoid cellsPreterm laborBackground Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveries. The ability of innate lymphoid cells (ILCs) to act as pro-inflammatory mediators arose the interest to study their role in normal and pathological pregnancies. The aim of this work was to analyze the relative frequencies of ILCs subsets in pregnancy and the levels of IL-4, IL-17, IL-22, and IFN-γ as inflammatory mediators. Accordingly, we hypothesized that changes in the proportions of ILCs subpopulations could be related to preterm birth. Methods We analyzed 15 full-term delivery samples and six preterm delivery samples. In the full-term group (FTB) peripheral blood was taken during routine blood analysis, on 3 occasions: 1st, 2nd and 3rd trimester. After delivery, peripheral blood, cord blood and placenta were collected. In PTB group, peripheral blood samples were obtained on two occasions: before and 24 h after treatment with progesterone. We used flow cytometry to analyze ILCs in maternal peripheral blood, placenta, and cord blood samples. Maternal peripheral blood and cord blood samples were analyzed by enzyme-linked immunosorbent assay for IL-4, IL-17, IL-22, and IFN-γ plasma levels at the time of labor. Results We observed significantly increased relative frequencies of ILC2 and ILC3 in the decidua, as well as an increase of ILC2 in cord blood samples in PTB group, compared to FTB samples. We also found a decrease in IFN-γ in peripheral blood samples of the PTB group, suggesting a functional withdrawal. Additionally, IL-4, IL-17, IL-22 levels were similar in PTB and FTB groups, denoting a relevant role in mediating labor. Conclusion Our results suggest that ILC2 and ILC3 play a role in PTB by mediating an inflammatory response. Further work is necessary to evaluate the importance of ILCs in the regulation of labor.F31D-D663-4EF2 | Anabela Mota PintoSpringer Nature2021-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/95690http://hdl.handle.net/10316/95690https://doi.org/10.1186/s12865-021-00423-xeng33957866PMC8101215Mendes, JoãoRodrigues-Santos, PauloAreia, Ana LuísaAlmeida, Jani-SofiaAlves, VeraSantos-Rosa, ManuelMota-Pinto, Anabelainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T03:12:20Zoai:estudogeral.uc.pt:10316/95690Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:14:06.274777Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
title |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
spellingShingle |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth Mendes, João Preterm birth Inflammation Innate immune response Innate lymphoid cells Preterm labor |
title_short |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
title_full |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
title_fullStr |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
title_full_unstemmed |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
title_sort |
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth |
author |
Mendes, João |
author_facet |
Mendes, João Rodrigues-Santos, Paulo Areia, Ana Luísa Almeida, Jani-Sofia Alves, Vera Santos-Rosa, Manuel Mota-Pinto, Anabela |
author_role |
author |
author2 |
Rodrigues-Santos, Paulo Areia, Ana Luísa Almeida, Jani-Sofia Alves, Vera Santos-Rosa, Manuel Mota-Pinto, Anabela |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mendes, João Rodrigues-Santos, Paulo Areia, Ana Luísa Almeida, Jani-Sofia Alves, Vera Santos-Rosa, Manuel Mota-Pinto, Anabela |
dc.subject.por.fl_str_mv |
Preterm birth Inflammation Innate immune response Innate lymphoid cells Preterm labor |
topic |
Preterm birth Inflammation Innate immune response Innate lymphoid cells Preterm labor |
description |
Background Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveries. The ability of innate lymphoid cells (ILCs) to act as pro-inflammatory mediators arose the interest to study their role in normal and pathological pregnancies. The aim of this work was to analyze the relative frequencies of ILCs subsets in pregnancy and the levels of IL-4, IL-17, IL-22, and IFN-γ as inflammatory mediators. Accordingly, we hypothesized that changes in the proportions of ILCs subpopulations could be related to preterm birth. Methods We analyzed 15 full-term delivery samples and six preterm delivery samples. In the full-term group (FTB) peripheral blood was taken during routine blood analysis, on 3 occasions: 1st, 2nd and 3rd trimester. After delivery, peripheral blood, cord blood and placenta were collected. In PTB group, peripheral blood samples were obtained on two occasions: before and 24 h after treatment with progesterone. We used flow cytometry to analyze ILCs in maternal peripheral blood, placenta, and cord blood samples. Maternal peripheral blood and cord blood samples were analyzed by enzyme-linked immunosorbent assay for IL-4, IL-17, IL-22, and IFN-γ plasma levels at the time of labor. Results We observed significantly increased relative frequencies of ILC2 and ILC3 in the decidua, as well as an increase of ILC2 in cord blood samples in PTB group, compared to FTB samples. We also found a decrease in IFN-γ in peripheral blood samples of the PTB group, suggesting a functional withdrawal. Additionally, IL-4, IL-17, IL-22 levels were similar in PTB and FTB groups, denoting a relevant role in mediating labor. Conclusion Our results suggest that ILC2 and ILC3 play a role in PTB by mediating an inflammatory response. Further work is necessary to evaluate the importance of ILCs in the regulation of labor. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/95690 http://hdl.handle.net/10316/95690 https://doi.org/10.1186/s12865-021-00423-x |
url |
http://hdl.handle.net/10316/95690 https://doi.org/10.1186/s12865-021-00423-x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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33957866 PMC8101215 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer Nature |
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Springer Nature |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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