Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability

Detalhes bibliográficos
Autor(a) principal: Cota, João Bettencourt
Data de Publicação: 2019
Outros Autores: Silva, Vanessa Ferreira da, Chambel, Lélia, Veloso, Maria Gabriela, Vieira-Pinto, Madalena, Oliveira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/21427
Resumo: Microbiology / Veterinary Sciences
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spelling Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence abilityCarcassContaminationPFGEPorkSalmonellaSlaughterhouseMicrobiology / Veterinary SciencesABSTRACT - Pork is one of the most common vehicles of non-typhoid foodborne Salmonella, with the slaughterhouse representing a key point for the infection of pigs and carcass contamination. By comparing matching samples taken from animals at the dirty (skin) and clean (inner and outer carcass surface) areas of the slaughterline, this study aimed to assess potential Salmonella contamination routes of pig carcasses within a Portuguese abattoir. Forty-four Salmonella isolates were retrieved from 120 pigs, and further characterized through pheno and genotypical methods. Most frequent serotypes found were Salmonella 4, [5],12:i:- (47.7%), Salmonella Rissen (40.9%) and Salmonella Derby (11.4%). Isolates were most commonly collected from the skin of pigs sampled at the dirty area (59.1%), followed by the inner (38.1%) and outer (9.1%) carcass surface sampled at the clean area. Most isolates (79.5%) were considered to be multidrug resistant and all harbored the virulence associated genes invA, invH, sopB, stn, slyA, phoP, phoQ and agfA. PFGE analysis revealed that most bacterial isolates belonging to the same serotype, recovered from animals from different farms, and slaughtered at separate days were genetically undistinguishable. Furthermore, our findings suggest that Salmonella Rissen might have an increased ability to endure on the slaughterhouse environment when compared with the other serotypes. Concluding, this study shows that the slaughterhouse may be a key point for the dissemination of resistant and virulent Salmonella strains, which stresses the importance of the implementation of good hygiene practices at the slaughterhouse and of the application of corrective measures to avoid cross-contamination.Elsevier B.V.Repositório da Universidade de LisboaCota, João BettencourtSilva, Vanessa Ferreira daChambel, LéliaVeloso, Maria GabrielaVieira-Pinto, MadalenaOliveira, Manuela2021-06-03T23:54:27Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21427engCota, João Bettencourt, Silva, Vanessa Ferreira da, Chambel, Lélia, Veloso, Maria Gabriela, Vieira-Pinto, Madalena, Oliveira, Manuela 2019. Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability [article]. Veterinary Microbiology, 239:108457. Doi:10.1016/j.vetmic.2019.1084571873254210.1016/j.vetmic.2019.108457info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:50:52Zoai:www.repository.utl.pt:10400.5/21427Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:01.030033Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
title Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
spellingShingle Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
Cota, João Bettencourt
Carcass
Contamination
PFGE
Pork
Salmonella
Slaughterhouse
title_short Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
title_full Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
title_fullStr Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
title_full_unstemmed Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
title_sort Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability
author Cota, João Bettencourt
author_facet Cota, João Bettencourt
Silva, Vanessa Ferreira da
Chambel, Lélia
Veloso, Maria Gabriela
Vieira-Pinto, Madalena
Oliveira, Manuela
author_role author
author2 Silva, Vanessa Ferreira da
Chambel, Lélia
Veloso, Maria Gabriela
Vieira-Pinto, Madalena
Oliveira, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cota, João Bettencourt
Silva, Vanessa Ferreira da
Chambel, Lélia
Veloso, Maria Gabriela
Vieira-Pinto, Madalena
Oliveira, Manuela
dc.subject.por.fl_str_mv Carcass
Contamination
PFGE
Pork
Salmonella
Slaughterhouse
topic Carcass
Contamination
PFGE
Pork
Salmonella
Slaughterhouse
description Microbiology / Veterinary Sciences
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2019-12-01T00:00:00Z
2021-06-03T23:54:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21427
url http://hdl.handle.net/10400.5/21427
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cota, João Bettencourt, Silva, Vanessa Ferreira da, Chambel, Lélia, Veloso, Maria Gabriela, Vieira-Pinto, Madalena, Oliveira, Manuela 2019. Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability [article]. Veterinary Microbiology, 239:108457. Doi:10.1016/j.vetmic.2019.108457
18732542
10.1016/j.vetmic.2019.108457
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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