Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/42052 |
Resumo: | The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and -glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs. |
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Valorizing recycled paper sludge by a bioethanol production process with cellulase recyclingRecycled paper sludgeCellulase recyclingUltrafiltrationAlkaline washCellulosic bioethanolScience & TechnologyThe feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and -glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs.The authors acknowledge the financial support of the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). The financial support of FCT through GlycoCBMs Project PTDC/AGR-FOR/3090/2012–FCOMP-01-0124-FEDER-027948 and the PhD grant to DG (SFRH/BD/88623/2012) is equally acknowledged. The authors also thank RENOVA (Portugal) for kindly providing the recycled paper sludge.ElsevierUniversidade do MinhoGomes, Daniel GonçalvesDomingues, LucíliaGama, F. M.2016-092016-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42052engGomes, Daniel G.; Domingues, Lucília; Gama, F. M., Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling. Bioresource Technology, 216, 637-644, 20160960-85240960-852410.1016/j.biortech.2016.06.00427289054http://www.journals.elsevier.com/bioresource-technology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:54:23Zoai:repositorium.sdum.uminho.pt:1822/42052Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:55.092376Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
title |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
spellingShingle |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling Gomes, Daniel Gonçalves Recycled paper sludge Cellulase recycling Ultrafiltration Alkaline wash Cellulosic bioethanol Science & Technology |
title_short |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
title_full |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
title_fullStr |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
title_full_unstemmed |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
title_sort |
Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling |
author |
Gomes, Daniel Gonçalves |
author_facet |
Gomes, Daniel Gonçalves Domingues, Lucília Gama, F. M. |
author_role |
author |
author2 |
Domingues, Lucília Gama, F. M. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gomes, Daniel Gonçalves Domingues, Lucília Gama, F. M. |
dc.subject.por.fl_str_mv |
Recycled paper sludge Cellulase recycling Ultrafiltration Alkaline wash Cellulosic bioethanol Science & Technology |
topic |
Recycled paper sludge Cellulase recycling Ultrafiltration Alkaline wash Cellulosic bioethanol Science & Technology |
description |
The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and -glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09 2016-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42052 |
url |
http://hdl.handle.net/1822/42052 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gomes, Daniel G.; Domingues, Lucília; Gama, F. M., Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling. Bioresource Technology, 216, 637-644, 2016 0960-8524 0960-8524 10.1016/j.biortech.2016.06.004 27289054 http://www.journals.elsevier.com/bioresource-technology/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133136926277632 |