A polyalanine peptide derived from polar fish with anti-infectious activities
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/37805 |
Resumo: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4 |
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A polyalanine peptide derived from polar fish with anti-infectious activitiesThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4Due to the growing concern about antibiotic-resistant microbial infections, increasing support has been given to new drug discovery programs. A promising alternative to counter bacterial infections includes the antimicrobial peptides (AMPs), which have emerged as model molecules for rational design strategies. Here we focused on the study of Pa-MAP 1.9, a rationally designed AMP derived from the polar fish Pleuronectes americanus. Pa-MAP 1.9 was active against Gram-negative planktonic bacteria and biofilms, without being cytotoxic to mammalian cells. By using AFM, leakage assays, CD spectroscopy and in silico tools, we found that Pa-MAP 1.9 may be acting both on intracellular targets and on the bacterial surface, also being more efficient at interacting with anionic LUVs mimicking Gram-negative bacterial surface, where this peptide adopts α-helical conformations, than cholesterolenriched LUVs mimicking mammalian cells. Thus, as bacteria present varied physiological features that favor antibiotic-resistance, Pa-MAP 1.9 could be a promising candidate in the development of tools against infections caused by pathogenic bacteria.Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number R21AI098701, and by a grant from the Canadian Institutes. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. R.E.W.H. holds a Canada Research Chair in Health and Genomics. C.D.L.F.-N. received a scholarship from the Fundación “la Caixa” and Fundación Canadá (Spain), and currently holds a postdoctoral scholarship from Fundación Ramón Areces (Spain). This work was also supported by Fundaçao para a Ciencia e a Tecnologia–Ministério da Educaçao e Ciencia (FCT-MEC, Portugal), including the fellowship SPRH/BD/100517/2014 to M.R.F., and by Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE, European Union) project INPACT (call H2020-MSCA-RISE-2014, grant agreement 644167). M.H.C., L.M. and O.L.F. acknowledge funding from CNPq, CAPES, FUNDECT and FAPDF.Nature ResearchRepositório da Universidade de LisboaCardoso, Marlon H.Ribeiro, Suzana M.Nolasco, Diego O.Fuente-Núñez, César de laFelício, Mário RomãoAbreu, Sónia GonçalvesMatos, Carolina O.Liao, Luciano M.Santos, Nuno C.Hancock, Robert E. W.Franco, Octávio L.Migliolo, Ludovico2019-04-02T11:27:31Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/37805engScientific Reports | 6:213852045-232210.1038/srep21385info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:35:09Zoai:repositorio.ul.pt:10451/37805Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:51:47.402233Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| title |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| spellingShingle |
A polyalanine peptide derived from polar fish with anti-infectious activities Cardoso, Marlon H. |
| title_short |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| title_full |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| title_fullStr |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| title_full_unstemmed |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| title_sort |
A polyalanine peptide derived from polar fish with anti-infectious activities |
| author |
Cardoso, Marlon H. |
| author_facet |
Cardoso, Marlon H. Ribeiro, Suzana M. Nolasco, Diego O. Fuente-Núñez, César de la Felício, Mário Romão Abreu, Sónia Gonçalves Matos, Carolina O. Liao, Luciano M. Santos, Nuno C. Hancock, Robert E. W. Franco, Octávio L. Migliolo, Ludovico |
| author_role |
author |
| author2 |
Ribeiro, Suzana M. Nolasco, Diego O. Fuente-Núñez, César de la Felício, Mário Romão Abreu, Sónia Gonçalves Matos, Carolina O. Liao, Luciano M. Santos, Nuno C. Hancock, Robert E. W. Franco, Octávio L. Migliolo, Ludovico |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Cardoso, Marlon H. Ribeiro, Suzana M. Nolasco, Diego O. Fuente-Núñez, César de la Felício, Mário Romão Abreu, Sónia Gonçalves Matos, Carolina O. Liao, Luciano M. Santos, Nuno C. Hancock, Robert E. W. Franco, Octávio L. Migliolo, Ludovico |
| description |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4 |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2019-04-02T11:27:31Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/37805 |
| url |
http://hdl.handle.net/10451/37805 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Scientific Reports | 6:21385 2045-2322 10.1038/srep21385 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Nature Research |
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Nature Research |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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