Biofabrication of Conductive Materials for Cardiac Regeneration

Detalhes bibliográficos
Autor(a) principal: Oliveira, Mariana Antunes de
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/62419
Resumo: Tese de mestrado, Engenharia Biomédica e Biofísica, 2023, Universidade de Lisboa, Faculdade de Ciências
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spelling Biofabrication of Conductive Materials for Cardiac RegenerationBioimpressãocardiomiócitosCNTsMXenosalginatoTeses de mestrado - 2023Departamento de FísicaTese de mestrado, Engenharia Biomédica e Biofísica, 2023, Universidade de Lisboa, Faculdade de CiênciasCardiovascular diseases (CVDs) are the leading cause of death worldwide. Heart donor shortage and cardiomyocyte poor self-renewal rate make it imperious to develop novel alternative therapies and approaches. To this end, Regenerative Medicine and Tissue Engineering (TE) have been playing an increasingly relevant role, and in particular 3D bioprinting techniques are a very promising solution as they allow to print human cells embedded in biocompatible materials in a controlled and precise fashion. However, a common problem found in cardiac cells bioprinting, is the lack of maturation of the printed or seeded cardiomyocytes. Moreover, nanomaterials with properties like electrical conductivity have been added to hydrogels to achieve properties suitable for cardiomyocyte culture. Hence, Alginate-Gelatin hydrogels containing either carbon nanotubes (CNTs), titanium carbide MXenes or no conductive nanomaterial were tested to be used as scaffolds for cell seeding and bioinks for bioprinting. Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CM) were seeded in 3 different hydrogel sheets (control - without particles -, with CNTs and with MXenes) and encapsulated in 3 bioinks with the same composition, for further extrusion bioprinting. Cell viability and metabolism were assessed. Primary tests regarding the conductivity and other relevant physical properties were conducted prior to cell culture. Findings suggested that both CNTs and MXenes have a positive effect on hiPSC-CMs metabolism without compromising cell viability. For seeded constructs, both conductive nanomaterials are thought to improve the maturation of hiPSC-CMs as after 6 days of culture there were clusters of cells displaying a mature phenotype. Yet, bioprinted hiPSC-CMs did not exhibit signs of maturity, as they did not spread along the hydrogel matrix and maintained a round shape, which is an indicator of low expression of contractile proteins. Although the obtained results are far from ideal, they configure a solid starting point for research on this topicMatela, Nuno Miguel de Pinto Lobo e, 1978-Almeida, HenriqueRepositório da Universidade de LisboaOliveira, Mariana Antunes de2024-02-01T18:27:02Z202320232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/62419enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-05T01:23:54Zoai:repositorio.ul.pt:10451/62419Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:08:31.259951Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofabrication of Conductive Materials for Cardiac Regeneration
title Biofabrication of Conductive Materials for Cardiac Regeneration
spellingShingle Biofabrication of Conductive Materials for Cardiac Regeneration
Oliveira, Mariana Antunes de
Bioimpressão
cardiomiócitos
CNTs
MXenos
alginato
Teses de mestrado - 2023
Departamento de Física
title_short Biofabrication of Conductive Materials for Cardiac Regeneration
title_full Biofabrication of Conductive Materials for Cardiac Regeneration
title_fullStr Biofabrication of Conductive Materials for Cardiac Regeneration
title_full_unstemmed Biofabrication of Conductive Materials for Cardiac Regeneration
title_sort Biofabrication of Conductive Materials for Cardiac Regeneration
author Oliveira, Mariana Antunes de
author_facet Oliveira, Mariana Antunes de
author_role author
dc.contributor.none.fl_str_mv Matela, Nuno Miguel de Pinto Lobo e, 1978-
Almeida, Henrique
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Oliveira, Mariana Antunes de
dc.subject.por.fl_str_mv Bioimpressão
cardiomiócitos
CNTs
MXenos
alginato
Teses de mestrado - 2023
Departamento de Física
topic Bioimpressão
cardiomiócitos
CNTs
MXenos
alginato
Teses de mestrado - 2023
Departamento de Física
description Tese de mestrado, Engenharia Biomédica e Biofísica, 2023, Universidade de Lisboa, Faculdade de Ciências
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023-01-01T00:00:00Z
2024-02-01T18:27:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/62419
url http://hdl.handle.net/10451/62419
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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