Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.26/30474 |
Resumo: | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spotsHIV-1 infectionAngolaDNA PCR assayDried blood spotsEarly infant diagnosisThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.BACKGROUND: Early diagnosis and treatment reduces HIV-1-related mortality, morbidity and size of viral reservoirs in infants infected perinatally. Commercial molecular tests enable the early diagnosis of infection in infants but the high cost and low sensitivity with dried blood spots (DBS) limit their use in sub-Saharan Africa. OBJECTIVES: To develop and validate a sensitive and cheap qualitative proviral DNA PCR-based assay for early infant diagnosis (EID) in HIV-1-exposed infants using DBS samples. STUDY DESIGN: Chelex-based method was used to extract DNA from DBS samples followed by a nested PCR assay using primers for the HIV-1 integrase gene. Limit of detection (LoD) was determined by Probit regression using limiting dilutions of newly produced recombinant plasmids with the integrase gene of all HIV-1 subtypes and ACH-2 cells. Clinical sensitivity and specificity were evaluated on 100 HIV-1 infected adults; 5 infected infants; 50 healthy volunteers; 139 HIV-1-exposed infants of the Angolan Pediatric HIV Cohort (APEHC) with serology at 18 months of life. RESULTS: All subtypes and CRF02_AG were amplified with a LoD of 14 copies. HIV-1 infection in infants was detected at month 1 of life. Sensitivity rate in adults varied with viral load, while diagnostic specificity was 100%. The percentage of HIV-1 MTCT cases between January 2012 and October 2014 was 2.2%. The cost per test was 8-10 USD which is 2- to 4-fold lower in comparison to commercial assays. CONCLUSIONS: The new PCR assay enables early and accurate EID. The simplicity and low-cost of the assay make it suitable for generalized implementation in Angola and other resource-constrained countries.PLoSRepositório ComumMartin, FranciscoPalladino, ClaudiaMateus, RitaBolzan, AnnaGomes, PerpétuaBrito, JoséCarvalho, Ana PatríciaCardoso, YolandaDomingos, CristóvãoClemente, Vanda Sofia LôaTaveira, Nuno2019-12-13T11:33:04Z2017-07-01T00:00:00Z2017-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/30474engMartin F, Palladino C, Mateus R, Bolzan A, Gomes P, Brito J, et al. (2017) Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots. PLoS ONE 12(7): e0181352. https://doi.org/10.1371/journal.pone.01813521932-620310.1371/journal.pone.0181352info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:53:52Zoai:comum.rcaap.pt:10400.26/30474Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:09:38.134049Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| title |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| spellingShingle |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots Martin, Francisco HIV-1 infection Angola DNA PCR assay Dried blood spots Early infant diagnosis |
| title_short |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| title_full |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| title_fullStr |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| title_full_unstemmed |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| title_sort |
Early infant diagnosis of HIV-1 infection in Luanda using a new DNA PCR test and dried blood spots |
| author |
Martin, Francisco |
| author_facet |
Martin, Francisco Palladino, Claudia Mateus, Rita Bolzan, Anna Gomes, Perpétua Brito, José Carvalho, Ana Patrícia Cardoso, Yolanda Domingos, Cristóvão Clemente, Vanda Sofia Lôa Taveira, Nuno |
| author_role |
author |
| author2 |
Palladino, Claudia Mateus, Rita Bolzan, Anna Gomes, Perpétua Brito, José Carvalho, Ana Patrícia Cardoso, Yolanda Domingos, Cristóvão Clemente, Vanda Sofia Lôa Taveira, Nuno |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Comum |
| dc.contributor.author.fl_str_mv |
Martin, Francisco Palladino, Claudia Mateus, Rita Bolzan, Anna Gomes, Perpétua Brito, José Carvalho, Ana Patrícia Cardoso, Yolanda Domingos, Cristóvão Clemente, Vanda Sofia Lôa Taveira, Nuno |
| dc.subject.por.fl_str_mv |
HIV-1 infection Angola DNA PCR assay Dried blood spots Early infant diagnosis |
| topic |
HIV-1 infection Angola DNA PCR assay Dried blood spots Early infant diagnosis |
| description |
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-07-01T00:00:00Z 2017-07-01T00:00:00Z 2019-12-13T11:33:04Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.26/30474 |
| url |
http://hdl.handle.net/10400.26/30474 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Martin F, Palladino C, Mateus R, Bolzan A, Gomes P, Brito J, et al. (2017) Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots. PLoS ONE 12(7): e0181352. https://doi.org/10.1371/journal.pone.0181352 1932-6203 10.1371/journal.pone.0181352 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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PLoS |
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PLoS |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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