SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.34629/ipl.isel.i-ETC.4 |
Resumo: | The transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALSSemiconductor Devices; Optoelectronics; ElectronicsFRET; biosensor; spectral response; pinpin photodiodeThe transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device.ISEL - High Institute of Engineering of Lisbon2013-06-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.34629/ipl.isel.i-ETC.4oai:i-ETC.journals.isel.pt:article/4i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-22182-4010reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://journals.isel.pt/index.php/i-ETC/article/view/4https://doi.org/10.34629/ipl.isel.i-ETC.4http://journals.isel.pt/index.php/i-ETC/article/view/4/4Copyright (c) 2013 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computershttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLouro, PaulaVieira, M.Vieira, M. A.Karmali, A.Fernades, M.2022-09-20T15:26:06Zoai:i-ETC.journals.isel.pt:article/4Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:51:12.452856Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
title |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
spellingShingle |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS Louro, Paula Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
title_short |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
title_full |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
title_fullStr |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
title_full_unstemmed |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
title_sort |
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
author |
Louro, Paula |
author_facet |
Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
author_role |
author |
author2 |
Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
dc.subject.por.fl_str_mv |
Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
topic |
Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
description |
The transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-27T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.34629/ipl.isel.i-ETC.4 oai:i-ETC.journals.isel.pt:article/4 |
url |
https://doi.org/10.34629/ipl.isel.i-ETC.4 |
identifier_str_mv |
oai:i-ETC.journals.isel.pt:article/4 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://journals.isel.pt/index.php/i-ETC/article/view/4 https://doi.org/10.34629/ipl.isel.i-ETC.4 http://journals.isel.pt/index.php/i-ETC/article/view/4/4 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2013 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2013 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ISEL - High Institute of Engineering of Lisbon |
publisher.none.fl_str_mv |
ISEL - High Institute of Engineering of Lisbon |
dc.source.none.fl_str_mv |
i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2 2182-4010 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130375508721664 |