Bacteriophage-encoded depolymerases: their diversity and biotechnological applications
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/40516 |
Resumo: | Bacteriophages (phages), natural enemies of bacteria, can encode enzymes able to degrade polymeric substances. These substances can be found in the bacterial cell surface, such as polysaccharides, or are produced by bacteria when they are living in biofilm communities, the most common bacterial lifestyle. Consequently, phages with depolymerase activity have a facilitated access to the host receptors, by degrading the capsular polysaccharides, and are believed to have a better performance against bacterial biofilms, since the degradation of extracellular polymeric substances by depolymerases might facilitate the access of phages to the cells within different biofilm layers. Since the diversity of phage depolymerases is not yet fully explored, this is the first review gathering information about all the depolymerases encoded by fully sequenced phages. Overall, in this study, 160 putative depolymerases, including sialidases, levanases, xylosidases, dextranases, hyaluronidases, peptidases as well as pectate/pectin lyases, were found in 143 phages (43 Myoviridae, 47 Siphoviridae, 37 Podoviridae, and 16 unclassified) infecting 24 genera of bacteria. We further provide information about the main applications of phage depolymerases, which can comprise areas as diverse as medical, chemical, or food-processing industry. |
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Bacteriophage-encoded depolymerases: their diversity and biotechnological applicationsBacteriophagesPhage depolymerasesCapsular polysaccharidesBiofilmsScience & TechnologyBacteriophages (phages), natural enemies of bacteria, can encode enzymes able to degrade polymeric substances. These substances can be found in the bacterial cell surface, such as polysaccharides, or are produced by bacteria when they are living in biofilm communities, the most common bacterial lifestyle. Consequently, phages with depolymerase activity have a facilitated access to the host receptors, by degrading the capsular polysaccharides, and are believed to have a better performance against bacterial biofilms, since the degradation of extracellular polymeric substances by depolymerases might facilitate the access of phages to the cells within different biofilm layers. Since the diversity of phage depolymerases is not yet fully explored, this is the first review gathering information about all the depolymerases encoded by fully sequenced phages. Overall, in this study, 160 putative depolymerases, including sialidases, levanases, xylosidases, dextranases, hyaluronidases, peptidases as well as pectate/pectin lyases, were found in 143 phages (43 Myoviridae, 47 Siphoviridae, 37 Podoviridae, and 16 unclassified) infecting 24 genera of bacteria. We further provide information about the main applications of phage depolymerases, which can comprise areas as diverse as medical, chemical, or food-processing industry.DPP acknowledges the financial support from the Portuguese Foundation for Science and Technology (FCT) through the grant SFRH/BD/76440/2011. SS is an FCT investigator (IF/01413/2013). The authors also thank FCT for the Strategic Project of the UID/BIO/04469/2013 unit, FCT and European Union funds (FEDER/COMPETE) for the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER027462).SpringerUniversidade do MinhoPires, Diana Priscila PensoOliveira, Hugo Alexandre MendesMelo, Luís D. R.Sillankorva, SannaAzeredo, Joana2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40516engPires, Diana; Oliveira, Hugo; Melo, L.; Sillankorva, Sanna; Azeredo, Joana, Bacteriophage-encoded depolymerases: their diversity and biotechnological applications. Applied Microbiology and Biotechnology, 100(5), 2141-2151, 20160175-75981432-061410.1007/s00253-015-7247-026767986http://www.springer.com/chemistry/biotechnology/journal/253info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:47Zoai:repositorium.sdum.uminho.pt:1822/40516Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:28.364780Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
title |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
spellingShingle |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications Pires, Diana Priscila Penso Bacteriophages Phage depolymerases Capsular polysaccharides Biofilms Science & Technology |
title_short |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
title_full |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
title_fullStr |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
title_full_unstemmed |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
title_sort |
Bacteriophage-encoded depolymerases: their diversity and biotechnological applications |
author |
Pires, Diana Priscila Penso |
author_facet |
Pires, Diana Priscila Penso Oliveira, Hugo Alexandre Mendes Melo, Luís D. R. Sillankorva, Sanna Azeredo, Joana |
author_role |
author |
author2 |
Oliveira, Hugo Alexandre Mendes Melo, Luís D. R. Sillankorva, Sanna Azeredo, Joana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pires, Diana Priscila Penso Oliveira, Hugo Alexandre Mendes Melo, Luís D. R. Sillankorva, Sanna Azeredo, Joana |
dc.subject.por.fl_str_mv |
Bacteriophages Phage depolymerases Capsular polysaccharides Biofilms Science & Technology |
topic |
Bacteriophages Phage depolymerases Capsular polysaccharides Biofilms Science & Technology |
description |
Bacteriophages (phages), natural enemies of bacteria, can encode enzymes able to degrade polymeric substances. These substances can be found in the bacterial cell surface, such as polysaccharides, or are produced by bacteria when they are living in biofilm communities, the most common bacterial lifestyle. Consequently, phages with depolymerase activity have a facilitated access to the host receptors, by degrading the capsular polysaccharides, and are believed to have a better performance against bacterial biofilms, since the degradation of extracellular polymeric substances by depolymerases might facilitate the access of phages to the cells within different biofilm layers. Since the diversity of phage depolymerases is not yet fully explored, this is the first review gathering information about all the depolymerases encoded by fully sequenced phages. Overall, in this study, 160 putative depolymerases, including sialidases, levanases, xylosidases, dextranases, hyaluronidases, peptidases as well as pectate/pectin lyases, were found in 143 phages (43 Myoviridae, 47 Siphoviridae, 37 Podoviridae, and 16 unclassified) infecting 24 genera of bacteria. We further provide information about the main applications of phage depolymerases, which can comprise areas as diverse as medical, chemical, or food-processing industry. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/40516 |
url |
http://hdl.handle.net/1822/40516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pires, Diana; Oliveira, Hugo; Melo, L.; Sillankorva, Sanna; Azeredo, Joana, Bacteriophage-encoded depolymerases: their diversity and biotechnological applications. Applied Microbiology and Biotechnology, 100(5), 2141-2151, 2016 0175-7598 1432-0614 10.1007/s00253-015-7247-0 26767986 http://www.springer.com/chemistry/biotechnology/journal/253 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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