An integrated BAC approach to the genomics of cork formation

Detalhes bibliográficos
Autor(a) principal: Pinto, Maria Joana Amado Teixeira
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/6107
Resumo: Mestrado em Biologia Funcional - Instituto Superior de Agronomia
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spelling An integrated BAC approach to the genomics of cork formationcorksuberinBAC libraryBESsFISHquantitative real time PCRMestrado em Biologia Funcional - Instituto Superior de AgronomiaCork is produced by Quercus suber, an evergreen oak with major economic and environmental importance in Portugal. Suberin is the main component of cork but little is known about the molecular processes underlying suberin biosynthesis. We screened a Quercus suber bacterial artificial chromosome (BAC) library for the presence of relevant genes for suberin biosynthesis through high-density BAC filters hybridizations and we validated hybridization positive clones by Colony PCR. We analyzed BAC-end sequences (BESs) to characterize the structure and composition of the cork oak genome. And we hybridized BAC DNA with mitotic cork oak chromosomes to obtain a cytogenetic map of Q. suber. Finally, we analyzed expression profiles of candidate genes of cork samples from cork oaks with different cork qualities, using quantitative real-time PCR. We identified several genes involved in suberin biosynthesis pathways. BESs analysis showed high identity with coding regions and suggest the existence of conserved regions within the Fagacea family. We found strong evidence that the genes Cyp 86A1 and GDSL involved in lipid suberin biosynthesis pathway are closely located in the genome. However, we did not succeed in mapping genes of interest in the cork oak genome and we did not find any relation between the genes relative expression and cork qualityThe work was partially supported by a grant from the Fundação para a Ciência e Tecnologia (FCT), project SuberGene (PTDC/AGR-GPL/101785/2008, Genomics of Cork Formation: an integrated approach of cork qualityISAPaiva, Jorge PintoCecílio, Leonor MoraisRepositório da Universidade de LisboaPinto, Maria Joana Amado Teixeira2013-11-06T15:41:23Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/6107TID:201847272engPinto, M.J.A.T. - An integrated BAC approach to the genomics of cork formation. Lisboa: ISA, 2013, 129 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:36:58Zoai:www.repository.utl.pt:10400.5/6107Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:53:32.583820Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An integrated BAC approach to the genomics of cork formation
title An integrated BAC approach to the genomics of cork formation
spellingShingle An integrated BAC approach to the genomics of cork formation
Pinto, Maria Joana Amado Teixeira
cork
suberin
BAC library
BESs
FISH
quantitative real time PCR
title_short An integrated BAC approach to the genomics of cork formation
title_full An integrated BAC approach to the genomics of cork formation
title_fullStr An integrated BAC approach to the genomics of cork formation
title_full_unstemmed An integrated BAC approach to the genomics of cork formation
title_sort An integrated BAC approach to the genomics of cork formation
author Pinto, Maria Joana Amado Teixeira
author_facet Pinto, Maria Joana Amado Teixeira
author_role author
dc.contributor.none.fl_str_mv Paiva, Jorge Pinto
Cecílio, Leonor Morais
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pinto, Maria Joana Amado Teixeira
dc.subject.por.fl_str_mv cork
suberin
BAC library
BESs
FISH
quantitative real time PCR
topic cork
suberin
BAC library
BESs
FISH
quantitative real time PCR
description Mestrado em Biologia Funcional - Instituto Superior de Agronomia
publishDate 2013
dc.date.none.fl_str_mv 2013-11-06T15:41:23Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/6107
TID:201847272
url http://hdl.handle.net/10400.5/6107
identifier_str_mv TID:201847272
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pinto, M.J.A.T. - An integrated BAC approach to the genomics of cork formation. Lisboa: ISA, 2013, 129 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131011251961856

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