Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5174 https://doi.org/10.1016/S0022-0728(02)00944-0 |
Resumo: | Adriamycin adsorbs strongly and irreversibly onto surfaces and this enabled electrochemical detection of in situ adriamycin oxidative damage to DNA. The adsorption of adriamycin onto glassy carbon and highly oriented pyrolytic graphite (HOPG) electrodes was studied by voltammetry and mode atomic force microscopy (MAC). At a glassy carbon electrode (GCE), the adsorbate has similar voltammetric behaviour to adriamycin in solution, which enabled the cyclic, differential pulse and square wave voltammetric study of the electron transfer reaction. The total surface concentration of adriamycin adsorbed onto GCE, from a 50 nM adriamycin solution during 3 min, was calculated to be 2.57×10-12 mol cm-2. The oxidation of adsorbed adriamycin is pH-dependent and corresponds to a two electron/two proton mechanism, and the detection limit for adriamycin adsorbed onto the GCE was 3.33×10-10 M. In situ AFM images show quick and spontaneous adsorption of the adriamycin onto a HOPG surface. Adriamycin forms a stable monolayer when adsorbed from different concentrations of adriamycin solutions and for short adsorption times. The strong and irreversible chemisorption of adriamycin onto carbon electrodes enables detection limits of the order of picomolar, which is much lower than the detection limits attainable by voltammetric methods for most organic compounds. |
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Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfacesVoltammetryAdriamycinDNA-biosensorAdsorptionElectrochemistryAdriamycin adsorbs strongly and irreversibly onto surfaces and this enabled electrochemical detection of in situ adriamycin oxidative damage to DNA. The adsorption of adriamycin onto glassy carbon and highly oriented pyrolytic graphite (HOPG) electrodes was studied by voltammetry and mode atomic force microscopy (MAC). At a glassy carbon electrode (GCE), the adsorbate has similar voltammetric behaviour to adriamycin in solution, which enabled the cyclic, differential pulse and square wave voltammetric study of the electron transfer reaction. The total surface concentration of adriamycin adsorbed onto GCE, from a 50 nM adriamycin solution during 3 min, was calculated to be 2.57×10-12 mol cm-2. The oxidation of adsorbed adriamycin is pH-dependent and corresponds to a two electron/two proton mechanism, and the detection limit for adriamycin adsorbed onto the GCE was 3.33×10-10 M. In situ AFM images show quick and spontaneous adsorption of the adriamycin onto a HOPG surface. Adriamycin forms a stable monolayer when adsorbed from different concentrations of adriamycin solutions and for short adsorption times. The strong and irreversible chemisorption of adriamycin onto carbon electrodes enables detection limits of the order of picomolar, which is much lower than the detection limits attainable by voltammetric methods for most organic compounds.http://www.sciencedirect.com/science/article/B6TGB-461XHJC-2/1/bf02acdf6451e90599fa676d325be3172002info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5174http://hdl.handle.net/10316/5174https://doi.org/10.1016/S0022-0728(02)00944-0engJournal of Electroanalytical Chemistry. 538-539:(2002) 267-276Brett, Ana Maria OliveiraPiedade, José António P.Paquim, Ana Maria Chiorceainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-12T10:21:31Zoai:estudogeral.uc.pt:10316/5174Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:20.432656Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
title |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
spellingShingle |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces Brett, Ana Maria Oliveira Voltammetry Adriamycin DNA-biosensor Adsorption Electrochemistry |
title_short |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
title_full |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
title_fullStr |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
title_full_unstemmed |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
title_sort |
Anodic voltammetry and AFM imaging of picomoles of adriamycin adsorbed onto carbon surfaces |
author |
Brett, Ana Maria Oliveira |
author_facet |
Brett, Ana Maria Oliveira Piedade, José António P. Paquim, Ana Maria Chiorcea |
author_role |
author |
author2 |
Piedade, José António P. Paquim, Ana Maria Chiorcea |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Brett, Ana Maria Oliveira Piedade, José António P. Paquim, Ana Maria Chiorcea |
dc.subject.por.fl_str_mv |
Voltammetry Adriamycin DNA-biosensor Adsorption Electrochemistry |
topic |
Voltammetry Adriamycin DNA-biosensor Adsorption Electrochemistry |
description |
Adriamycin adsorbs strongly and irreversibly onto surfaces and this enabled electrochemical detection of in situ adriamycin oxidative damage to DNA. The adsorption of adriamycin onto glassy carbon and highly oriented pyrolytic graphite (HOPG) electrodes was studied by voltammetry and mode atomic force microscopy (MAC). At a glassy carbon electrode (GCE), the adsorbate has similar voltammetric behaviour to adriamycin in solution, which enabled the cyclic, differential pulse and square wave voltammetric study of the electron transfer reaction. The total surface concentration of adriamycin adsorbed onto GCE, from a 50 nM adriamycin solution during 3 min, was calculated to be 2.57×10-12 mol cm-2. The oxidation of adsorbed adriamycin is pH-dependent and corresponds to a two electron/two proton mechanism, and the detection limit for adriamycin adsorbed onto the GCE was 3.33×10-10 M. In situ AFM images show quick and spontaneous adsorption of the adriamycin onto a HOPG surface. Adriamycin forms a stable monolayer when adsorbed from different concentrations of adriamycin solutions and for short adsorption times. The strong and irreversible chemisorption of adriamycin onto carbon electrodes enables detection limits of the order of picomolar, which is much lower than the detection limits attainable by voltammetric methods for most organic compounds. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5174 http://hdl.handle.net/10316/5174 https://doi.org/10.1016/S0022-0728(02)00944-0 |
url |
http://hdl.handle.net/10316/5174 https://doi.org/10.1016/S0022-0728(02)00944-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Electroanalytical Chemistry. 538-539:(2002) 267-276 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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