Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/4734 https://doi.org/10.1016/j.ejca.2006.03.033 |
Resumo: | The aim of this study is to evaluate epidermal growth factor receptor variant III, EGFRvIII, a cancer specific mutant, as a possible marker for the diagnosis of breast cancer occult systemic disease. EGFRvIII mRNA was identified by an RT-nested PCR with a high sensitivity. In 102 women studied, the mutant was detected in the peripheral blood of 30% of 33 low risk, early stage patients, in 56% of 18 patients selected for neoadjuvant chemotherapy, in 63.6% of 11 patients with disseminated disease and 0% of 40 control women. In low risk, early stage patients, the presence of one or more tumour characteristics predicting recurrence such as the absence of oestrogen receptors and the presence of ERBB2 or histologic grades G2/G3 was significantly associated with EFGRvIII detection (p < 0.05). EGFRvIII mRNA has characteristics to be a useful marker for the diagnosis of occult systemic disease in breast cancer. Follow-up studies will evaluate its clinical value as a decision criterion for systemic therapy. |
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Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patientsEGFRvIIIBreast cancerOccult systemic diseaseThe aim of this study is to evaluate epidermal growth factor receptor variant III, EGFRvIII, a cancer specific mutant, as a possible marker for the diagnosis of breast cancer occult systemic disease. EGFRvIII mRNA was identified by an RT-nested PCR with a high sensitivity. In 102 women studied, the mutant was detected in the peripheral blood of 30% of 33 low risk, early stage patients, in 56% of 18 patients selected for neoadjuvant chemotherapy, in 63.6% of 11 patients with disseminated disease and 0% of 40 control women. In low risk, early stage patients, the presence of one or more tumour characteristics predicting recurrence such as the absence of oestrogen receptors and the presence of ERBB2 or histologic grades G2/G3 was significantly associated with EFGRvIII detection (p < 0.05). EGFRvIII mRNA has characteristics to be a useful marker for the diagnosis of occult systemic disease in breast cancer. Follow-up studies will evaluate its clinical value as a decision criterion for systemic therapy.http://www.sciencedirect.com/science/article/B6T68-4KV2RH2-1/1/8d7f06700e09e0cb34c8a3861e1b0bab2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/4734http://hdl.handle.net/10316/4734https://doi.org/10.1016/j.ejca.2006.03.033engEuropean Journal of Cancer. 42:15 (2006) 2617-2622Silva, Henriqueta A. C.Abraúl, ElsaRaimundo, DulceDias, Margarida F.Marques, CarlosGuerra, CarlosOliveira, Carlos F. deRegateiro, Fernando J.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-27T10:09:03Zoai:estudogeral.uc.pt:10316/4734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:33.683106Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
title |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
spellingShingle |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients Silva, Henriqueta A. C. EGFRvIII Breast cancer Occult systemic disease |
title_short |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
title_full |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
title_fullStr |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
title_full_unstemmed |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
title_sort |
Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients |
author |
Silva, Henriqueta A. C. |
author_facet |
Silva, Henriqueta A. C. Abraúl, Elsa Raimundo, Dulce Dias, Margarida F. Marques, Carlos Guerra, Carlos Oliveira, Carlos F. de Regateiro, Fernando J. |
author_role |
author |
author2 |
Abraúl, Elsa Raimundo, Dulce Dias, Margarida F. Marques, Carlos Guerra, Carlos Oliveira, Carlos F. de Regateiro, Fernando J. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Henriqueta A. C. Abraúl, Elsa Raimundo, Dulce Dias, Margarida F. Marques, Carlos Guerra, Carlos Oliveira, Carlos F. de Regateiro, Fernando J. |
dc.subject.por.fl_str_mv |
EGFRvIII Breast cancer Occult systemic disease |
topic |
EGFRvIII Breast cancer Occult systemic disease |
description |
The aim of this study is to evaluate epidermal growth factor receptor variant III, EGFRvIII, a cancer specific mutant, as a possible marker for the diagnosis of breast cancer occult systemic disease. EGFRvIII mRNA was identified by an RT-nested PCR with a high sensitivity. In 102 women studied, the mutant was detected in the peripheral blood of 30% of 33 low risk, early stage patients, in 56% of 18 patients selected for neoadjuvant chemotherapy, in 63.6% of 11 patients with disseminated disease and 0% of 40 control women. In low risk, early stage patients, the presence of one or more tumour characteristics predicting recurrence such as the absence of oestrogen receptors and the presence of ERBB2 or histologic grades G2/G3 was significantly associated with EFGRvIII detection (p < 0.05). EGFRvIII mRNA has characteristics to be a useful marker for the diagnosis of occult systemic disease in breast cancer. Follow-up studies will evaluate its clinical value as a decision criterion for systemic therapy. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/4734 http://hdl.handle.net/10316/4734 https://doi.org/10.1016/j.ejca.2006.03.033 |
url |
http://hdl.handle.net/10316/4734 https://doi.org/10.1016/j.ejca.2006.03.033 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Cancer. 42:15 (2006) 2617-2622 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133707776294912 |