Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process

Detalhes bibliográficos
Autor(a) principal: Palatsi, J.
Data de Publicação: 2012
Outros Autores: Affes, R., Fernandez, B., Pereira, M. A., Alves, M. M., Flotats, X.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22323
Resumo: The impact of LCFA adsorption on the methanogenic activity was evaluated in batch assays for two anaerobic granular sludges in the presence and absence of bentonite as synthetic adsorbent. A clear inhibitory effect at an oleate (C18:1) concentration of 0.5 g(C18:1) L(-1) was observed for both sludges. Palmitate (C16:0) was confirmed to be the main intermediate of C18:1 degradation in not adapted sludge and its accumulation was further evidenced by fluorescence staining and microscopy techniques. LCFA inhibition could be decreased by the addition of bentonite, reducing the lag-phase and accelerating the kinetics of LCFA degradation, concluding in the importance of the adsorptive nature of the LCFA inhibitory process. Granule morphology and molecular profiling of predominant microorganisms revealed that biomass adaptation to LCFA could modify the intermediates accumulation profiles and process rates.
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spelling Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition processAnaerobic granular sludgeLCFA inhibitionAdsorption isotherms16S rDNA profilingMicroscopic techniquesScience & TechnologyThe impact of LCFA adsorption on the methanogenic activity was evaluated in batch assays for two anaerobic granular sludges in the presence and absence of bentonite as synthetic adsorbent. A clear inhibitory effect at an oleate (C18:1) concentration of 0.5 g(C18:1) L(-1) was observed for both sludges. Palmitate (C16:0) was confirmed to be the main intermediate of C18:1 degradation in not adapted sludge and its accumulation was further evidenced by fluorescence staining and microscopy techniques. LCFA inhibition could be decreased by the addition of bentonite, reducing the lag-phase and accelerating the kinetics of LCFA degradation, concluding in the importance of the adsorptive nature of the LCFA inhibitory process. Granule morphology and molecular profiling of predominant microorganisms revealed that biomass adaptation to LCFA could modify the intermediates accumulation profiles and process rates.This work was funded by the Spanish Ministry of Science and Innovation (projects ENE 2007-65850 and CTM 2010-18212), and was partially supported by a grand from the Department of Universities, Research and Media Society of Catalonia Government (BE-DGR 2008 BE1 00261). We would like to thank Lucia Neves, Ana Nicolau, Madalena Vieira, and Ana Julia Cavaleiro, from University of Minho, for their assistance in microscopic observations and analytical methods. We also thank Miriam Guivernau (IRTA) for assistance in the PCR-DGGE profiling and ribotype sequencing. We are also grateful to David Bedoya (MWH) and Francesc Prenafeta (IRTA) for the revision and critical reading of the manuscript.ElsevierUniversidade do MinhoPalatsi, J.Affes, R.Fernandez, B.Pereira, M. A.Alves, M. M.Flotats, X.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22323eng0043-135410.1016/j.watres.2012.07.00822841596info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:07Zoai:repositorium.sdum.uminho.pt:1822/22323Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:27.895091Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
title Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
spellingShingle Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
Palatsi, J.
Anaerobic granular sludge
LCFA inhibition
Adsorption isotherms
16S rDNA profiling
Microscopic techniques
Science & Technology
title_short Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
title_full Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
title_fullStr Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
title_full_unstemmed Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
title_sort Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process
author Palatsi, J.
author_facet Palatsi, J.
Affes, R.
Fernandez, B.
Pereira, M. A.
Alves, M. M.
Flotats, X.
author_role author
author2 Affes, R.
Fernandez, B.
Pereira, M. A.
Alves, M. M.
Flotats, X.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Palatsi, J.
Affes, R.
Fernandez, B.
Pereira, M. A.
Alves, M. M.
Flotats, X.
dc.subject.por.fl_str_mv Anaerobic granular sludge
LCFA inhibition
Adsorption isotherms
16S rDNA profiling
Microscopic techniques
Science & Technology
topic Anaerobic granular sludge
LCFA inhibition
Adsorption isotherms
16S rDNA profiling
Microscopic techniques
Science & Technology
description The impact of LCFA adsorption on the methanogenic activity was evaluated in batch assays for two anaerobic granular sludges in the presence and absence of bentonite as synthetic adsorbent. A clear inhibitory effect at an oleate (C18:1) concentration of 0.5 g(C18:1) L(-1) was observed for both sludges. Palmitate (C16:0) was confirmed to be the main intermediate of C18:1 degradation in not adapted sludge and its accumulation was further evidenced by fluorescence staining and microscopy techniques. LCFA inhibition could be decreased by the addition of bentonite, reducing the lag-phase and accelerating the kinetics of LCFA degradation, concluding in the importance of the adsorptive nature of the LCFA inhibitory process. Granule morphology and molecular profiling of predominant microorganisms revealed that biomass adaptation to LCFA could modify the intermediates accumulation profiles and process rates.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22323
url http://hdl.handle.net/1822/22323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0043-1354
10.1016/j.watres.2012.07.008
22841596
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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