The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/56270 |
Resumo: | Gold nanoparticles (AuNPs) enable the treatment and real-time monitoring of several diseases, providing an exciting and advantageous nanomedicine strategy. These NPs have therefore been adequately functionalized to enable them to carry growth factors (GF), namely basic fibroblastic (bF) GF, which play an essential role in different and important cellular processes including cellular proliferation, survival, migration and differentiation. The AuNPs were coated with natural polymers, chitosan and heparin, to enhance their physicochemical properties such as suspension stability. The polyelectrolyte coating was monitored using a quartz crystal microbalance with dissipation, size and zeta-potential analysis. The natural polymer-coated AuNPs have a spherical shape and a positive surface charge due to chitosan amino groups, enabling their biofunctionalization with monoclonal antibodies to target specific biomolecules. Additionally, cellular assays with the chondrocyte cell line ATDC5 show that the NPs are cytocompatible at relevant concentrations. As a proof of concept of their potential application in tissue regeneration, the natural polymer-coated AuNPs were further functionalized with an antibody to selectively bind the desired GF. The bFGF concentration reached in the NPs without compromising the cytocompatibility demonstrates the potential of this carrier for tissue regeneration. |
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The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regenerationbasic fibroblastic growth factorGold nanoparticleslayer-by-layer technologynatural polymersScience & TechnologyGold nanoparticles (AuNPs) enable the treatment and real-time monitoring of several diseases, providing an exciting and advantageous nanomedicine strategy. These NPs have therefore been adequately functionalized to enable them to carry growth factors (GF), namely basic fibroblastic (bF) GF, which play an essential role in different and important cellular processes including cellular proliferation, survival, migration and differentiation. The AuNPs were coated with natural polymers, chitosan and heparin, to enhance their physicochemical properties such as suspension stability. The polyelectrolyte coating was monitored using a quartz crystal microbalance with dissipation, size and zeta-potential analysis. The natural polymer-coated AuNPs have a spherical shape and a positive surface charge due to chitosan amino groups, enabling their biofunctionalization with monoclonal antibodies to target specific biomolecules. Additionally, cellular assays with the chondrocyte cell line ATDC5 show that the NPs are cytocompatible at relevant concentrations. As a proof of concept of their potential application in tissue regeneration, the natural polymer-coated AuNPs were further functionalized with an antibody to selectively bind the desired GF. The bFGF concentration reached in the NPs without compromising the cytocompatibility demonstrates the potential of this carrier for tissue regeneration.The authors acknowledge QREN (project ‘‘RL2-SCN-NORTE-070124-FEDER-000018’’ co-financed by the North Portugal Regional Operational Programme (ON.2, O Novo Norte) under the NSRF through the ERDF), Portuguese Foundation for Science and Technology (project PTDC/CTM-BIO/4388/2014-SPARTAN) and European Research Council grant agreement ERC-2012ADG-20120216-321266 – project ComplexiTE for financing this research work. Authors would also like to thank the Portuguese Foundation for Science and Technology for the Post-Doc grant of Marta Alves da Silva (SFRH/BPD/73322/2010, financed by POPH– QREN–Tipologia 4.1– Advanced Formation, co-financed by Fundo Social Europeu and MEC national funds).info:eu-repo/semantics/publishedVersionRoyal Society of ChemistryUniversidade do MinhoFerreira, H.Martins, A.Silva, M. L. A.Amorim, S.Faria, S.Pires, R. A.Reis, R. L.Neves, N. M.2018-032018-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56270engFerreira H., Martins A., Silva M. L. A., Amorim S., Faria S., Pires R. A., Reis R. L., Neves N. M. The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration, J. Mater. Chem. B, Vol. 6, Issue 14, pp. 2104-2115, doi:10.1039/C7TB03273K, 20182050-750X10.1039/C7TB03273K32254433http://www.rsc.org/journals-books-databases/about-journals/journal-of-materials-chemistry-b/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:13Zoai:repositorium.sdum.uminho.pt:1822/56270Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:29.202532Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
title |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
spellingShingle |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration Ferreira, H. basic fibroblastic growth factor Gold nanoparticles layer-by-layer technology natural polymers Science & Technology |
title_short |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
title_full |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
title_fullStr |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
title_full_unstemmed |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
title_sort |
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration |
author |
Ferreira, H. |
author_facet |
Ferreira, H. Martins, A. Silva, M. L. A. Amorim, S. Faria, S. Pires, R. A. Reis, R. L. Neves, N. M. |
author_role |
author |
author2 |
Martins, A. Silva, M. L. A. Amorim, S. Faria, S. Pires, R. A. Reis, R. L. Neves, N. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ferreira, H. Martins, A. Silva, M. L. A. Amorim, S. Faria, S. Pires, R. A. Reis, R. L. Neves, N. M. |
dc.subject.por.fl_str_mv |
basic fibroblastic growth factor Gold nanoparticles layer-by-layer technology natural polymers Science & Technology |
topic |
basic fibroblastic growth factor Gold nanoparticles layer-by-layer technology natural polymers Science & Technology |
description |
Gold nanoparticles (AuNPs) enable the treatment and real-time monitoring of several diseases, providing an exciting and advantageous nanomedicine strategy. These NPs have therefore been adequately functionalized to enable them to carry growth factors (GF), namely basic fibroblastic (bF) GF, which play an essential role in different and important cellular processes including cellular proliferation, survival, migration and differentiation. The AuNPs were coated with natural polymers, chitosan and heparin, to enhance their physicochemical properties such as suspension stability. The polyelectrolyte coating was monitored using a quartz crystal microbalance with dissipation, size and zeta-potential analysis. The natural polymer-coated AuNPs have a spherical shape and a positive surface charge due to chitosan amino groups, enabling their biofunctionalization with monoclonal antibodies to target specific biomolecules. Additionally, cellular assays with the chondrocyte cell line ATDC5 show that the NPs are cytocompatible at relevant concentrations. As a proof of concept of their potential application in tissue regeneration, the natural polymer-coated AuNPs were further functionalized with an antibody to selectively bind the desired GF. The bFGF concentration reached in the NPs without compromising the cytocompatibility demonstrates the potential of this carrier for tissue regeneration. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03 2018-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/56270 |
url |
http://hdl.handle.net/1822/56270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreira H., Martins A., Silva M. L. A., Amorim S., Faria S., Pires R. A., Reis R. L., Neves N. M. The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration, J. Mater. Chem. B, Vol. 6, Issue 14, pp. 2104-2115, doi:10.1039/C7TB03273K, 2018 2050-750X 10.1039/C7TB03273K 32254433 http://www.rsc.org/journals-books-databases/about-journals/journal-of-materials-chemistry-b/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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