Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70876 |
Resumo: | The aim of this study was to evaluate the behavior of different lipid-based nanostructures during in vitro digestion, in particular on curcumins bioaccessibility, and to access their potential toxicity. Solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC) and nanoemulsions (NE) were submitted to harmonized static in vitro digestion and their cytotoxicity and cellular transport were evaluated using Caco-2 cell line. NE presented the highest curcumins bioaccessibility followed by NLC and SLN, 71.1%, 63.7% and 53.3%, respectively. Free fatty acids percentage increased in the following order: NLC ? NE < SLN. Non-digested nanostructures and excipients presented no cytotoxicity; however, digested NE and NLC presented cytotoxicity due to MCT oil, which presented cytotoxicity after digestion. The apparent permeability coefficient of NLC was higher than SLN and NE. These results showed that lipid-based nanostructures physical state and composition have a high influence on particles' behavior during digestion, and on their cytotoxicity/intestinal permeability, and highlights the importance of conducting cytotoxicity assessments after in vitro digestion. This work contributes to a better understanding of the behavior of lipid-based nanostructures under digestion/adsorption, and this knowledge will be useful in design of nanostructures that afford both safety and an increased bioactive compounds bioavailability. |
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Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessmentNanoemulsionsNanostructured lipid carriersSolid lipid nanoparticlesstatic digestionIntestinal permeabilityLipid physical stateIn vitro static digestionScience & TechnologyThe aim of this study was to evaluate the behavior of different lipid-based nanostructures during in vitro digestion, in particular on curcumins bioaccessibility, and to access their potential toxicity. Solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC) and nanoemulsions (NE) were submitted to harmonized static in vitro digestion and their cytotoxicity and cellular transport were evaluated using Caco-2 cell line. NE presented the highest curcumins bioaccessibility followed by NLC and SLN, 71.1%, 63.7% and 53.3%, respectively. Free fatty acids percentage increased in the following order: NLC ? NE < SLN. Non-digested nanostructures and excipients presented no cytotoxicity; however, digested NE and NLC presented cytotoxicity due to MCT oil, which presented cytotoxicity after digestion. The apparent permeability coefficient of NLC was higher than SLN and NE. These results showed that lipid-based nanostructures physical state and composition have a high influence on particles' behavior during digestion, and on their cytotoxicity/intestinal permeability, and highlights the importance of conducting cytotoxicity assessments after in vitro digestion. This work contributes to a better understanding of the behavior of lipid-based nanostructures under digestion/adsorption, and this knowledge will be useful in design of nanostructures that afford both safety and an increased bioactive compounds bioavailability.Acknowledgments Raquel F. S. Goncalves acknowledge the Foundation for Science and Technology (FCT) for her fellowship (SFRH/BD/140182/2018) . This study was supported by Foundation for Science and Technology (FCT) under the scope of Project PTDC/AGRTEC/5215/2014, the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020Programa Operacional Regional do Norte. Funding from INTERFACE Programme through the Innovation, Technology and Circular Economy Fund (FITEC) and iNova4Health, a program also financially supported by Fundacao para a Ciencia eTecnologia, is also gratefully acknowledged.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoGonçalves, Raquel Filipa SilvaMartins, Joana Teresa RodriguesAbrunhosa, LuísBaixinho, JoãoMatias, Ana A.Vicente, A. A.Pinheiro, A. C.20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70876engGonçalves, Raquel F. S.; Martins, Joana T.; Abrunhosa, Luís; Baixinho, João; Matias, Ana A.; Vicente, António A.; Pinheiro, Ana Cristina, Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment. Food Research International, 143(110278), 20210963-996910.1016/j.foodres.2021.11027833992378http://www.journals.elsevier.com/food-research-international/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:52Zoai:repositorium.sdum.uminho.pt:1822/70876Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:59.730873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
title |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
spellingShingle |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment Gonçalves, Raquel Filipa Silva Nanoemulsions Nanostructured lipid carriers Solid lipid nanoparticles static digestion Intestinal permeability Lipid physical state In vitro static digestion Science & Technology |
title_short |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
title_full |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
title_fullStr |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
title_full_unstemmed |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
title_sort |
Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment |
author |
Gonçalves, Raquel Filipa Silva |
author_facet |
Gonçalves, Raquel Filipa Silva Martins, Joana Teresa Rodrigues Abrunhosa, Luís Baixinho, João Matias, Ana A. Vicente, A. A. Pinheiro, A. C. |
author_role |
author |
author2 |
Martins, Joana Teresa Rodrigues Abrunhosa, Luís Baixinho, João Matias, Ana A. Vicente, A. A. Pinheiro, A. C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gonçalves, Raquel Filipa Silva Martins, Joana Teresa Rodrigues Abrunhosa, Luís Baixinho, João Matias, Ana A. Vicente, A. A. Pinheiro, A. C. |
dc.subject.por.fl_str_mv |
Nanoemulsions Nanostructured lipid carriers Solid lipid nanoparticles static digestion Intestinal permeability Lipid physical state In vitro static digestion Science & Technology |
topic |
Nanoemulsions Nanostructured lipid carriers Solid lipid nanoparticles static digestion Intestinal permeability Lipid physical state In vitro static digestion Science & Technology |
description |
The aim of this study was to evaluate the behavior of different lipid-based nanostructures during in vitro digestion, in particular on curcumins bioaccessibility, and to access their potential toxicity. Solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC) and nanoemulsions (NE) were submitted to harmonized static in vitro digestion and their cytotoxicity and cellular transport were evaluated using Caco-2 cell line. NE presented the highest curcumins bioaccessibility followed by NLC and SLN, 71.1%, 63.7% and 53.3%, respectively. Free fatty acids percentage increased in the following order: NLC ? NE < SLN. Non-digested nanostructures and excipients presented no cytotoxicity; however, digested NE and NLC presented cytotoxicity due to MCT oil, which presented cytotoxicity after digestion. The apparent permeability coefficient of NLC was higher than SLN and NE. These results showed that lipid-based nanostructures physical state and composition have a high influence on particles' behavior during digestion, and on their cytotoxicity/intestinal permeability, and highlights the importance of conducting cytotoxicity assessments after in vitro digestion. This work contributes to a better understanding of the behavior of lipid-based nanostructures under digestion/adsorption, and this knowledge will be useful in design of nanostructures that afford both safety and an increased bioactive compounds bioavailability. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70876 |
url |
http://hdl.handle.net/1822/70876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gonçalves, Raquel F. S.; Martins, Joana T.; Abrunhosa, Luís; Baixinho, João; Matias, Ana A.; Vicente, António A.; Pinheiro, Ana Cristina, Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment. Food Research International, 143(110278), 2021 0963-9969 10.1016/j.foodres.2021.110278 33992378 http://www.journals.elsevier.com/food-research-international/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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