Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.4/2222 |
Resumo: | Type 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardioprotective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [(14)C]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P= 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P< 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment. |
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Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetesAdipócitosTecido AdiposoDiabetes Mellitus Tipo 2Insuficiência CardíacaLipóliseType 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardioprotective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [(14)C]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P= 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P< 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment.RIHUCBurgeiro, AFuhrmann, ACherian, SEspinoza, DJarak, ICarvalho, RALoureiro, MPatrício, MAntunes, MJCarvalho, E2019-05-22T16:07:20Z2016-04-012016-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/2222engAm J Physiol Endocrinol Metab. 2016 Apr 1;310(7):E550-64.10.1152/ajpendo.00384.2015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:23:36Zoai:rihuc.huc.min-saude.pt:10400.4/2222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:04:41.197718Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
title |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
spellingShingle |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes Burgeiro, A Adipócitos Tecido Adiposo Diabetes Mellitus Tipo 2 Insuficiência Cardíaca Lipólise |
title_short |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
title_full |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
title_fullStr |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
title_full_unstemmed |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
title_sort |
Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes |
author |
Burgeiro, A |
author_facet |
Burgeiro, A Fuhrmann, A Cherian, S Espinoza, D Jarak, I Carvalho, RA Loureiro, M Patrício, M Antunes, MJ Carvalho, E |
author_role |
author |
author2 |
Fuhrmann, A Cherian, S Espinoza, D Jarak, I Carvalho, RA Loureiro, M Patrício, M Antunes, MJ Carvalho, E |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RIHUC |
dc.contributor.author.fl_str_mv |
Burgeiro, A Fuhrmann, A Cherian, S Espinoza, D Jarak, I Carvalho, RA Loureiro, M Patrício, M Antunes, MJ Carvalho, E |
dc.subject.por.fl_str_mv |
Adipócitos Tecido Adiposo Diabetes Mellitus Tipo 2 Insuficiência Cardíaca Lipólise |
topic |
Adipócitos Tecido Adiposo Diabetes Mellitus Tipo 2 Insuficiência Cardíaca Lipólise |
description |
Type 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardioprotective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [(14)C]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P= 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P< 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 2016-04-01T00:00:00Z 2019-05-22T16:07:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.4/2222 |
url |
http://hdl.handle.net/10400.4/2222 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Am J Physiol Endocrinol Metab. 2016 Apr 1;310(7):E550-64. 10.1152/ajpendo.00384.2015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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