Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10437/12146 |
Resumo: | The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology. |
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Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharidesBIOTECNOLOGIABIOQUÍMICABIOQUÍMICA ALIMENTARBIOTECHNOLOGYBIOCHEMISTRYFOOD BIOCHEMISTRYThe crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.Springer2021-09-06T11:37:17Z2021-01-01T00:00:00Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10437/12146eng10.1007/s10068-021-00931-xRibeiro, Geise Camila de AraújoFernandes, Pedro Carlos de BarrosSilva, Dayse Alessandra AlmeidaBrandão, Hugo NevesAssis, Sandra Aparecida deinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:07:10Zoai:recil.ensinolusofona.pt:10437/12146Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:14:35.162334Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
title |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
spellingShingle |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides Ribeiro, Geise Camila de Araújo BIOTECNOLOGIA BIOQUÍMICA BIOQUÍMICA ALIMENTAR BIOTECHNOLOGY BIOCHEMISTRY FOOD BIOCHEMISTRY |
title_short |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
title_full |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
title_fullStr |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
title_full_unstemmed |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
title_sort |
Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides |
author |
Ribeiro, Geise Camila de Araújo |
author_facet |
Ribeiro, Geise Camila de Araújo Fernandes, Pedro Carlos de Barros Silva, Dayse Alessandra Almeida Brandão, Hugo Neves Assis, Sandra Aparecida de |
author_role |
author |
author2 |
Fernandes, Pedro Carlos de Barros Silva, Dayse Alessandra Almeida Brandão, Hugo Neves Assis, Sandra Aparecida de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Geise Camila de Araújo Fernandes, Pedro Carlos de Barros Silva, Dayse Alessandra Almeida Brandão, Hugo Neves Assis, Sandra Aparecida de |
dc.subject.por.fl_str_mv |
BIOTECNOLOGIA BIOQUÍMICA BIOQUÍMICA ALIMENTAR BIOTECHNOLOGY BIOCHEMISTRY FOOD BIOCHEMISTRY |
topic |
BIOTECNOLOGIA BIOQUÍMICA BIOQUÍMICA ALIMENTAR BIOTECHNOLOGY BIOCHEMISTRY FOOD BIOCHEMISTRY |
description |
The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-06T11:37:17Z 2021-01-01T00:00:00Z 2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10437/12146 |
url |
http://hdl.handle.net/10437/12146 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1007/s10068-021-00931-x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131242843602944 |