Quantification of the CBD-FITC conjugates surface coating on cellulose fibres

Detalhes bibliográficos
Autor(a) principal: Pinto, João Ricardo
Data de Publicação: 2008
Outros Autores: Amaral, A. L., Ferreira, Eugénio C., Mota, M., Vilanova, Manuel, Ruel, Katia, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/7735
Resumo: Background Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).
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spelling Quantification of the CBD-FITC conjugates surface coating on cellulose fibresScience & TechnologyBackground Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/6934/2001.BioMed Central (BMC)Universidade do MinhoPinto, João RicardoAmaral, A. L.Ferreira, Eugénio C.Mota, M.Vilanova, ManuelRuel, KatiaGama, F. M.20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/7735eng"BMC Biotechnology". ISSN 1472-6750. 8:2 (2008).1472-675010.1186/1472-6750-8-118184429This article is available from: http://www.biomedcentral.com/1472-6750/8/1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:34Zoai:repositorium.sdum.uminho.pt:1822/7735Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:45.109919Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
title Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
spellingShingle Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
Pinto, João Ricardo
Science & Technology
title_short Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
title_full Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
title_fullStr Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
title_full_unstemmed Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
title_sort Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
author Pinto, João Ricardo
author_facet Pinto, João Ricardo
Amaral, A. L.
Ferreira, Eugénio C.
Mota, M.
Vilanova, Manuel
Ruel, Katia
Gama, F. M.
author_role author
author2 Amaral, A. L.
Ferreira, Eugénio C.
Mota, M.
Vilanova, Manuel
Ruel, Katia
Gama, F. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pinto, João Ricardo
Amaral, A. L.
Ferreira, Eugénio C.
Mota, M.
Vilanova, Manuel
Ruel, Katia
Gama, F. M.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Background Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/7735
url https://hdl.handle.net/1822/7735
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "BMC Biotechnology". ISSN 1472-6750. 8:2 (2008).
1472-6750
10.1186/1472-6750-8-1
18184429
This article is available from: http://www.biomedcentral.com/1472-6750/8/1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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