Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy
| Autor(a) principal: | |
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| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/58208 |
Resumo: | Copyright © 2021 Carmona-Fernandes, Barreira, Leonardo, Casimiro, Castro, Santos, Fernandes, Cortes-Figueiredo, Gonçalves, Cruz, Fernandes, Ivo, Pedro, Canhão, Fonseca and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomyAtherosclerosisBone remodeling biomarkersOsteoporosisPro-inflammatory cytokinesTissue expression analysisCopyright © 2021 Carmona-Fernandes, Barreira, Leonardo, Casimiro, Castro, Santos, Fernandes, Cortes-Figueiredo, Gonçalves, Cruz, Fernandes, Ivo, Pedro, Canhão, Fonseca and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Background and Aims: Atherosclerosis and osteoporosis share common risk factors, as well as inflammatory mechanisms. Our aim was to understand how atherosclerotic lesions are related with disturbances in bone. Methods: Gene expression of pro-inflammatory and bone metabolism related proteins (IL-1β, IL-6, IL-17A, TNF, RANKL, OPG, COL1, CTSK, OCL, TRAP, CBFA1, DKK1, SOST, ADIPOQ, and ADIPOR1) were analyzed in arteries and bones from 45 deceased donors and adipose tissue was used as control. Additionally, in 139 patients with advanced atherosclerosis submitted to carotid endarterectomy we compared calcium content (Alizarin red) and plaque inflammatory scores (CD3+, CD68+, and adiponectin) of patients with normal bone mineral density (BMD) with those with low BMD and explored the associations between gene expression in atherosclerotic plaques and BMD. Serum levels of pro-inflammatory and bone related proteins were measured both in donors and patients. Associations were investigated by the Pearson or Spearman correlation tests, and multivariate regression analyzes were performed when justified. Results: Gene expression of bone remodeling and pro-inflammatory proteins correlated positively in bone and aorta, independently of age and sex of donors, but not in adipose tissue. The expression of bone formation genes was significantly higher in atheroma plaques from endarterectomized patients with normal vs. low BMD as well as inflammatory CD68+ scores, regardless of patients' age and sex, but not of body mass index. No relationship was observed between serum levels and gene expression levels of pro-inflammatory or bone remodeling proteins. Conclusions: Our results suggest that the relationship between bones and vessels in the context of atherosclerotic disease and osteoporosis may rely on the intrinsic connection between the tissues involved, independently of disease stage. Serum measurements of pro-inflammatory and bone-remodeling proteins do not accurately translate tissue pathologic processes.FrontiersRepositório da Universidade de LisboaCarmona-Fernandes, DianaBarreira, SofiaLeonardo, NatachaCasimiro, Renata I.Castro, Alice M.Santos, Pedro OliveiraFernandes, António N.Cortes-Figueiredo, FilipeGonçalves, Carolina A.Cruz, Rafael Moiteiro DaFernandes, Mariana L.Ivo, MargaridaPedro, Luís MCanhao, HelenaFonseca, João EuricoSantos, Maria2023-06-14T14:24:08Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/58208engFront Med (Lausanne). 2021 May 20;8:67249610.3389/fmed.2021.6724962296-858Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:06:56Zoai:repositorio.ul.pt:10451/58208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:08:30.790564Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| title |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| spellingShingle |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy Carmona-Fernandes, Diana Atherosclerosis Bone remodeling biomarkers Osteoporosis Pro-inflammatory cytokines Tissue expression analysis |
| title_short |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| title_full |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| title_fullStr |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| title_full_unstemmed |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| title_sort |
Atherosclerosis and bone loss in humans: results from deceased donors and from patients submitted to carotid endarterectomy |
| author |
Carmona-Fernandes, Diana |
| author_facet |
Carmona-Fernandes, Diana Barreira, Sofia Leonardo, Natacha Casimiro, Renata I. Castro, Alice M. Santos, Pedro Oliveira Fernandes, António N. Cortes-Figueiredo, Filipe Gonçalves, Carolina A. Cruz, Rafael Moiteiro Da Fernandes, Mariana L. Ivo, Margarida Pedro, Luís M Canhao, Helena Fonseca, João Eurico Santos, Maria |
| author_role |
author |
| author2 |
Barreira, Sofia Leonardo, Natacha Casimiro, Renata I. Castro, Alice M. Santos, Pedro Oliveira Fernandes, António N. Cortes-Figueiredo, Filipe Gonçalves, Carolina A. Cruz, Rafael Moiteiro Da Fernandes, Mariana L. Ivo, Margarida Pedro, Luís M Canhao, Helena Fonseca, João Eurico Santos, Maria |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Carmona-Fernandes, Diana Barreira, Sofia Leonardo, Natacha Casimiro, Renata I. Castro, Alice M. Santos, Pedro Oliveira Fernandes, António N. Cortes-Figueiredo, Filipe Gonçalves, Carolina A. Cruz, Rafael Moiteiro Da Fernandes, Mariana L. Ivo, Margarida Pedro, Luís M Canhao, Helena Fonseca, João Eurico Santos, Maria |
| dc.subject.por.fl_str_mv |
Atherosclerosis Bone remodeling biomarkers Osteoporosis Pro-inflammatory cytokines Tissue expression analysis |
| topic |
Atherosclerosis Bone remodeling biomarkers Osteoporosis Pro-inflammatory cytokines Tissue expression analysis |
| description |
Copyright © 2021 Carmona-Fernandes, Barreira, Leonardo, Casimiro, Castro, Santos, Fernandes, Cortes-Figueiredo, Gonçalves, Cruz, Fernandes, Ivo, Pedro, Canhão, Fonseca and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2023-06-14T14:24:08Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/58208 |
| url |
http://hdl.handle.net/10451/58208 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Front Med (Lausanne). 2021 May 20;8:672496 10.3389/fmed.2021.672496 2296-858X |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Frontiers |
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Frontiers |
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