Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers

Detalhes bibliográficos
Autor(a) principal: Pinto, João Ricardo
Data de Publicação: 2007
Outros Autores: Amaral, A. L., Carvalho, Joana, Ferreira, Eugénio C., Mota, M., Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/7319
Resumo: The surface concentration of CBD-FITC conjugates, adsorbed on cellulose fibers, was determined by image analysis. The program consists of two scripts, the first dedicated to the elaboration of the calibration curve. The emission of fluorescent light, detected by image analysis, is correlated with the concentration of CBD solutions. This calibration is then used (second script) to determine the concentration of CBDs adsorbed on cellulosic fibers. This method allows the direct estimation of the surface concentration of adsorbed CBDs, which usually is not accurately calculated from depletion studies, since the surface area is hardly known. By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs.
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spelling Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibersScience & TechnologyThe surface concentration of CBD-FITC conjugates, adsorbed on cellulose fibers, was determined by image analysis. The program consists of two scripts, the first dedicated to the elaboration of the calibration curve. The emission of fluorescent light, detected by image analysis, is correlated with the concentration of CBD solutions. This calibration is then used (second script) to determine the concentration of CBDs adsorbed on cellulosic fibers. This method allows the direct estimation of the surface concentration of adsorbed CBDs, which usually is not accurately calculated from depletion studies, since the surface area is hardly known. By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs.Fundação para a Ciência e a Tecnologia (FCT)American Chemical SocietyAmerican Institute of Chemical EngineersUniversidade do MinhoPinto, João RicardoAmaral, A. L.Carvalho, JoanaFerreira, Eugénio C.Mota, M.Gama, F. M.2007-122007-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/7319eng"Biotechnology Progress". ISSN 8756-7938. 23:6 (Dec. 2007) 1492-1497.8756-793810.1021/bp070026v17988144info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:18Zoai:repositorium.sdum.uminho.pt:1822/7319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:32.220609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
title Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
spellingShingle Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
Pinto, João Ricardo
Science & Technology
title_short Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
title_full Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
title_fullStr Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
title_full_unstemmed Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
title_sort Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
author Pinto, João Ricardo
author_facet Pinto, João Ricardo
Amaral, A. L.
Carvalho, Joana
Ferreira, Eugénio C.
Mota, M.
Gama, F. M.
author_role author
author2 Amaral, A. L.
Carvalho, Joana
Ferreira, Eugénio C.
Mota, M.
Gama, F. M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pinto, João Ricardo
Amaral, A. L.
Carvalho, Joana
Ferreira, Eugénio C.
Mota, M.
Gama, F. M.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description The surface concentration of CBD-FITC conjugates, adsorbed on cellulose fibers, was determined by image analysis. The program consists of two scripts, the first dedicated to the elaboration of the calibration curve. The emission of fluorescent light, detected by image analysis, is correlated with the concentration of CBD solutions. This calibration is then used (second script) to determine the concentration of CBDs adsorbed on cellulosic fibers. This method allows the direct estimation of the surface concentration of adsorbed CBDs, which usually is not accurately calculated from depletion studies, since the surface area is hardly known. By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
2007-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/7319
url https://hdl.handle.net/1822/7319
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biotechnology Progress". ISSN 8756-7938. 23:6 (Dec. 2007) 1492-1497.
8756-7938
10.1021/bp070026v
17988144
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
American Institute of Chemical Engineers
publisher.none.fl_str_mv American Chemical Society
American Institute of Chemical Engineers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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