Development of a platelet lysates-based hydrogel for nerve regeneration
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/30911 |
Resumo: | Spinal cord injury (SCI) is a life-changing and debilitating medical condition. Currently, there is no definitive treatment. Novel regenerative medicine therapies have emerged in order to treat SCI. In this field, hydrogels are promising tools due to its high versatility of applications and design. Brain tissue has some particular characteristics such as its viscoelastic microenvironment and its soft extracellular matrix (ECM). Hydrogels can be designed to suit these characteristics and, therefore, be used to mimic brain tissue. Herein, we investigated the potential of a methacryloyl platelet lysates (PLMA) hydrogel to be applied as scaffold for axonal regeneration or as a carrier of cells. We observed that neurons do not adhere easily to the surface of the PLMA hydrogel alone. However, when the gel is coated with adhesives proteins like laminin or poly-D-lysine we observed that a large number of neurons adhere to its surface. Moreover, our data suggests that PLMA100 20% hydrogel can be used as a carrier of cortical neurons, which is of great relevance given that it can be used to replaced dead neurons. Additionally, adipose-derived stem cells (ASCs) are appealing cells to be encapsulated in PLMA100 hydrogels due to their effects on neurites outgrowth. In order to accomplish functional recovery, it is required that axonal regeneration be followed by synapse formation. However, ASCs’ synaptogenic potencial continues undetermined. In line with this, we analyzed the ability of ASCs to promote synapse formation. Our results indicate that ASCs are capable of inducing presynaptic clusters, a hallmark of synapse formation. PLMA hydrogel might possibly be used as a scaffold for axonal regeneration and a carrier for neuronal cells, making it a strong contender for development of future novel neuro-regenerative therapies. |
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Development of a platelet lysates-based hydrogel for nerve regenerationRegenerative medicineHydrogelsSpinal cord injuryAxonal regenerationCortical neuronsHippocampal neuronsAdipose-derived stem cellsSynapseSpinal cord injury (SCI) is a life-changing and debilitating medical condition. Currently, there is no definitive treatment. Novel regenerative medicine therapies have emerged in order to treat SCI. In this field, hydrogels are promising tools due to its high versatility of applications and design. Brain tissue has some particular characteristics such as its viscoelastic microenvironment and its soft extracellular matrix (ECM). Hydrogels can be designed to suit these characteristics and, therefore, be used to mimic brain tissue. Herein, we investigated the potential of a methacryloyl platelet lysates (PLMA) hydrogel to be applied as scaffold for axonal regeneration or as a carrier of cells. We observed that neurons do not adhere easily to the surface of the PLMA hydrogel alone. However, when the gel is coated with adhesives proteins like laminin or poly-D-lysine we observed that a large number of neurons adhere to its surface. Moreover, our data suggests that PLMA100 20% hydrogel can be used as a carrier of cortical neurons, which is of great relevance given that it can be used to replaced dead neurons. Additionally, adipose-derived stem cells (ASCs) are appealing cells to be encapsulated in PLMA100 hydrogels due to their effects on neurites outgrowth. In order to accomplish functional recovery, it is required that axonal regeneration be followed by synapse formation. However, ASCs’ synaptogenic potencial continues undetermined. In line with this, we analyzed the ability of ASCs to promote synapse formation. Our results indicate that ASCs are capable of inducing presynaptic clusters, a hallmark of synapse formation. PLMA hydrogel might possibly be used as a scaffold for axonal regeneration and a carrier for neuronal cells, making it a strong contender for development of future novel neuro-regenerative therapies.A lesão da medula espinhal é uma condição médica debilitante que transforma negativamente a vida dos pacientes. Atualmente, não há um tratamento eficaz. Por esta razão, novas terapias de medicina regenerativa surgiram com o intuito de tratar esta condição. Nesta área, os hidrogéis são ferramentas promissoras devido à sua versatilidade de aplicações e design. O tecido cerebral tem caraterísticas particulares, como o seu microambiente viscoelástico e a sua matriz extracelular suave/mole. Os hidrogéis podem ser produzidos de forma a cumprir estas caraterísticas e, assim, serem usados de forma a mimetizar o tecido cerebral. Neste trabalho investigámos o potencial do hidrogel de metacriloílo e lisados de plaquetas (PLMA) ser aplicado como um scaffold para regeneração axonal ou como um transportador de células. Observámos que os neurónios não aderiram facilmente à superfície do hidrogel PLMA. No entanto, quando o gel é revestido com proteínas adesivas como a lamina ou a poli-D lisina (PDL) observámos que um maior número de neurónios aderiu à sua superfície. Além disso, os nossos resultados sugerem que o hidrogel PLMA100 20% pode ser usado como um transportador de neurónios corticais, o que é de grande relevância uma vez que pode ser usado para substituir neurónios degenerados. Adicionalmente, as células mesenquimais derivadas do tecido adiposo (ASCs) são células atrativas para serem encapsuladas nos hidrogéis PLMA100 devido aos seus efeitos no crescimento de neurites. De forma a obter uma recuperação funcional, é necessário que a regeneração axonal seja seguida por formação de sinapses. No entanto, o potencial sinaptogénico das ASCs continua inexplorado. Deste modo, analisámos a capacidade das ASCs de promover a formação de sinapses. Os nossos resultados indicam que as ASCs são capazes de induzir clusters pré-sinápticos, uma das principais caraterísticas da formação de sinapses. O hidrogel PLMA poderá possivelmente ser usado como um scaffold para regeneração axonal e um transportador para células neuronais, tornando-o um forte candidato no desenvolvimento de futuras terapêuticas neuro-regenerativas.2025-03-06T00:00:00Z2020-02-22T00:00:00Z2020-02-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30911engCorreia, Joana Filipa Ribeiroinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:59:45Zoai:ria.ua.pt:10773/30911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:55.962897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of a platelet lysates-based hydrogel for nerve regeneration |
title |
Development of a platelet lysates-based hydrogel for nerve regeneration |
spellingShingle |
Development of a platelet lysates-based hydrogel for nerve regeneration Correia, Joana Filipa Ribeiro Regenerative medicine Hydrogels Spinal cord injury Axonal regeneration Cortical neurons Hippocampal neurons Adipose-derived stem cells Synapse |
title_short |
Development of a platelet lysates-based hydrogel for nerve regeneration |
title_full |
Development of a platelet lysates-based hydrogel for nerve regeneration |
title_fullStr |
Development of a platelet lysates-based hydrogel for nerve regeneration |
title_full_unstemmed |
Development of a platelet lysates-based hydrogel for nerve regeneration |
title_sort |
Development of a platelet lysates-based hydrogel for nerve regeneration |
author |
Correia, Joana Filipa Ribeiro |
author_facet |
Correia, Joana Filipa Ribeiro |
author_role |
author |
dc.contributor.author.fl_str_mv |
Correia, Joana Filipa Ribeiro |
dc.subject.por.fl_str_mv |
Regenerative medicine Hydrogels Spinal cord injury Axonal regeneration Cortical neurons Hippocampal neurons Adipose-derived stem cells Synapse |
topic |
Regenerative medicine Hydrogels Spinal cord injury Axonal regeneration Cortical neurons Hippocampal neurons Adipose-derived stem cells Synapse |
description |
Spinal cord injury (SCI) is a life-changing and debilitating medical condition. Currently, there is no definitive treatment. Novel regenerative medicine therapies have emerged in order to treat SCI. In this field, hydrogels are promising tools due to its high versatility of applications and design. Brain tissue has some particular characteristics such as its viscoelastic microenvironment and its soft extracellular matrix (ECM). Hydrogels can be designed to suit these characteristics and, therefore, be used to mimic brain tissue. Herein, we investigated the potential of a methacryloyl platelet lysates (PLMA) hydrogel to be applied as scaffold for axonal regeneration or as a carrier of cells. We observed that neurons do not adhere easily to the surface of the PLMA hydrogel alone. However, when the gel is coated with adhesives proteins like laminin or poly-D-lysine we observed that a large number of neurons adhere to its surface. Moreover, our data suggests that PLMA100 20% hydrogel can be used as a carrier of cortical neurons, which is of great relevance given that it can be used to replaced dead neurons. Additionally, adipose-derived stem cells (ASCs) are appealing cells to be encapsulated in PLMA100 hydrogels due to their effects on neurites outgrowth. In order to accomplish functional recovery, it is required that axonal regeneration be followed by synapse formation. However, ASCs’ synaptogenic potencial continues undetermined. In line with this, we analyzed the ability of ASCs to promote synapse formation. Our results indicate that ASCs are capable of inducing presynaptic clusters, a hallmark of synapse formation. PLMA hydrogel might possibly be used as a scaffold for axonal regeneration and a carrier for neuronal cells, making it a strong contender for development of future novel neuro-regenerative therapies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-22T00:00:00Z 2020-02-22 2025-03-06T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/30911 |
url |
http://hdl.handle.net/10773/30911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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