Comparative transcriptomics of Arabidopsis sperm cells
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/104 |
Resumo: | n flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization are crucial goals in the study of plant reproduction. Studies of gene expression in male gametes of maize (Zea mays) and Plumbago and in lily (Lilium longiflorum) generative cells already showed that the previously held view of transcriptionally inert male gametes was not true, but genome-wide studies were lacking. Analyses in the model plant Arabidopsis (Arabidopsis thaliana) were hindered, because no method to isolate sperm cells was available. Here, we used fluorescence-activated cell sorting to isolate sperm cells from Arabidopsis, allowing GeneChip analysis of their transcriptome at a genome-wide level. Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has a distinct and diverse transcriptional profile. Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitin-mediated proteolysis, and cell cycle progression are overrepresented Gene Ontology categories. Moreover, analysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome. We identified numerous candidate genes whose involvement in sperm cell development and fertilization can now be directly tested in Arabidopsis. These results provide a roadmap to decipher the role of sperm-expressed proteins |
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Comparative transcriptomics of Arabidopsis sperm cellsLONGIFLORUM GENERATIVE CELLSPOLLEN-TUBE GUIDANCEF-BOX PROTEINSn flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization are crucial goals in the study of plant reproduction. Studies of gene expression in male gametes of maize (Zea mays) and Plumbago and in lily (Lilium longiflorum) generative cells already showed that the previously held view of transcriptionally inert male gametes was not true, but genome-wide studies were lacking. Analyses in the model plant Arabidopsis (Arabidopsis thaliana) were hindered, because no method to isolate sperm cells was available. Here, we used fluorescence-activated cell sorting to isolate sperm cells from Arabidopsis, allowing GeneChip analysis of their transcriptome at a genome-wide level. Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has a distinct and diverse transcriptional profile. Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitin-mediated proteolysis, and cell cycle progression are overrepresented Gene Ontology categories. Moreover, analysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome. We identified numerous candidate genes whose involvement in sperm cell development and fertilization can now be directly tested in Arabidopsis. These results provide a roadmap to decipher the role of sperm-expressed proteinsAmerican Society of Plant PhysiologistsARCABorges, Filipe FilipeGomes, GabrielaGardner, RuiMoreno, NunoMcCormick, SheilaFeijo, Jose A. Jose A.Becker, Joeg D Joeg D2010-04-21T15:52:41Z2008-102008-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/104engBorges, F., Gomes, M.G.M., Gardner, R., Moreno, N., McCormick, S., Feijó, J.A., Becker, J.D. (2008). "Comparative Transcriptomics of Arabidopsis Sperm Cells." Plant Physiology 148: 1-14.0032-0889info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:38Zoai:arca.igc.gulbenkian.pt:10400.7/104Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:34.797081Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative transcriptomics of Arabidopsis sperm cells |
title |
Comparative transcriptomics of Arabidopsis sperm cells |
spellingShingle |
Comparative transcriptomics of Arabidopsis sperm cells Borges, Filipe Filipe LONGIFLORUM GENERATIVE CELLS POLLEN-TUBE GUIDANCE F-BOX PROTEINS |
title_short |
Comparative transcriptomics of Arabidopsis sperm cells |
title_full |
Comparative transcriptomics of Arabidopsis sperm cells |
title_fullStr |
Comparative transcriptomics of Arabidopsis sperm cells |
title_full_unstemmed |
Comparative transcriptomics of Arabidopsis sperm cells |
title_sort |
Comparative transcriptomics of Arabidopsis sperm cells |
author |
Borges, Filipe Filipe |
author_facet |
Borges, Filipe Filipe Gomes, Gabriela Gardner, Rui Moreno, Nuno McCormick, Sheila Feijo, Jose A. Jose A. Becker, Joeg D Joeg D |
author_role |
author |
author2 |
Gomes, Gabriela Gardner, Rui Moreno, Nuno McCormick, Sheila Feijo, Jose A. Jose A. Becker, Joeg D Joeg D |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Borges, Filipe Filipe Gomes, Gabriela Gardner, Rui Moreno, Nuno McCormick, Sheila Feijo, Jose A. Jose A. Becker, Joeg D Joeg D |
dc.subject.por.fl_str_mv |
LONGIFLORUM GENERATIVE CELLS POLLEN-TUBE GUIDANCE F-BOX PROTEINS |
topic |
LONGIFLORUM GENERATIVE CELLS POLLEN-TUBE GUIDANCE F-BOX PROTEINS |
description |
n flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization are crucial goals in the study of plant reproduction. Studies of gene expression in male gametes of maize (Zea mays) and Plumbago and in lily (Lilium longiflorum) generative cells already showed that the previously held view of transcriptionally inert male gametes was not true, but genome-wide studies were lacking. Analyses in the model plant Arabidopsis (Arabidopsis thaliana) were hindered, because no method to isolate sperm cells was available. Here, we used fluorescence-activated cell sorting to isolate sperm cells from Arabidopsis, allowing GeneChip analysis of their transcriptome at a genome-wide level. Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has a distinct and diverse transcriptional profile. Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitin-mediated proteolysis, and cell cycle progression are overrepresented Gene Ontology categories. Moreover, analysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome. We identified numerous candidate genes whose involvement in sperm cell development and fertilization can now be directly tested in Arabidopsis. These results provide a roadmap to decipher the role of sperm-expressed proteins |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10 2008-10-01T00:00:00Z 2010-04-21T15:52:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/104 |
url |
http://hdl.handle.net/10400.7/104 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Borges, F., Gomes, M.G.M., Gardner, R., Moreno, N., McCormick, S., Feijó, J.A., Becker, J.D. (2008). "Comparative Transcriptomics of Arabidopsis Sperm Cells." Plant Physiology 148: 1-14. 0032-0889 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Plant Physiologists |
publisher.none.fl_str_mv |
American Society of Plant Physiologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130571434098688 |