Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/23937 |
Resumo: | The results confirm UE-ME3 strain as a Crabtree-positive yeast, since the addition of glucose to respiratory cells caused an increase of cell viability, alcoholic fermentative and glycolytic activities, repression of citrate cycle and respiratory chain, followed by a decrease in cell damages. The exposure of S. cerevisiae to TiO2-NP with a molecular diameter less than 100 nm under heat shock (28/40 °C), for 200 min has prevent the glucose-dependent transition from respiratory to respiratory-fermentative metabolism, as evidenced by maintenance of ADH enzyme activity near of detected in respiratory cells, as well as by lowest levels of cell viability, glycolytic, fermentative, and antioxidant activities and highest levels of oxidative stress, cell damages and more active respiratory metabolism. |
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Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae.YeastTiO2-NPenergy metabolismrespiratory metabolismThe results confirm UE-ME3 strain as a Crabtree-positive yeast, since the addition of glucose to respiratory cells caused an increase of cell viability, alcoholic fermentative and glycolytic activities, repression of citrate cycle and respiratory chain, followed by a decrease in cell damages. The exposure of S. cerevisiae to TiO2-NP with a molecular diameter less than 100 nm under heat shock (28/40 °C), for 200 min has prevent the glucose-dependent transition from respiratory to respiratory-fermentative metabolism, as evidenced by maintenance of ADH enzyme activity near of detected in respiratory cells, as well as by lowest levels of cell viability, glycolytic, fermentative, and antioxidant activities and highest levels of oxidative stress, cell damages and more active respiratory metabolism.SciKnowledge2019-01-09T14:36:59Z2019-01-092018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/23937http://hdl.handle.net/10174/23937engCapela-Pires J, Ferreira R, Alves-Pereira I (2018) Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. 4th International Conference on Green Chemistry and Sustainable Engineering (GreenChem-18) 23-25 July 2018 Madrid, Spain, EU978-84-09-03323-2jmcp@uevora.ptraf@uevora.ptiap@uevora.ptCapela-Pires, JFerreira, RAlves-Pereira, Iinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:16:32Zoai:dspace.uevora.pt:10174/23937Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:41.477938Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
title |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
spellingShingle |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. Capela-Pires, J Yeast TiO2-NP energy metabolism respiratory metabolism |
title_short |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
title_full |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
title_fullStr |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
title_full_unstemmed |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
title_sort |
Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. |
author |
Capela-Pires, J |
author_facet |
Capela-Pires, J Ferreira, R Alves-Pereira, I |
author_role |
author |
author2 |
Ferreira, R Alves-Pereira, I |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Capela-Pires, J Ferreira, R Alves-Pereira, I |
dc.subject.por.fl_str_mv |
Yeast TiO2-NP energy metabolism respiratory metabolism |
topic |
Yeast TiO2-NP energy metabolism respiratory metabolism |
description |
The results confirm UE-ME3 strain as a Crabtree-positive yeast, since the addition of glucose to respiratory cells caused an increase of cell viability, alcoholic fermentative and glycolytic activities, repression of citrate cycle and respiratory chain, followed by a decrease in cell damages. The exposure of S. cerevisiae to TiO2-NP with a molecular diameter less than 100 nm under heat shock (28/40 °C), for 200 min has prevent the glucose-dependent transition from respiratory to respiratory-fermentative metabolism, as evidenced by maintenance of ADH enzyme activity near of detected in respiratory cells, as well as by lowest levels of cell viability, glycolytic, fermentative, and antioxidant activities and highest levels of oxidative stress, cell damages and more active respiratory metabolism. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01T00:00:00Z 2019-01-09T14:36:59Z 2019-01-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/23937 http://hdl.handle.net/10174/23937 |
url |
http://hdl.handle.net/10174/23937 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Capela-Pires J, Ferreira R, Alves-Pereira I (2018) Titanium dioxide nanoparticles under heat-shock negatively modulate the Crabtree effect in Saccharomyces cerevisiae. 4th International Conference on Green Chemistry and Sustainable Engineering (GreenChem-18) 23-25 July 2018 Madrid, Spain, EU 978-84-09-03323-2 jmcp@uevora.pt raf@uevora.pt iap@uevora.pt |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
SciKnowledge |
publisher.none.fl_str_mv |
SciKnowledge |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136628405436416 |