β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/9268 |
Resumo: | β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane- Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline (mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI) using glutaraldehyde as activating agent being the synthesis of GOS evaluated and compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by fitting the experimental data were compared in order to determine the effect of the immobilization process with different supports on the synthesis of oligosaccharides. These results clearly demonstrate that all supports may be used for β-galactosidase immobilization as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation from the obtained reaction products is easy to accomplish. |
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β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides productionβ-galactosidaseImmobilizationMagnetic supportsGOSMathematical modelingβ-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane- Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline (mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI) using glutaraldehyde as activating agent being the synthesis of GOS evaluated and compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by fitting the experimental data were compared in order to determine the effect of the immobilization process with different supports on the synthesis of oligosaccharides. These results clearly demonstrate that all supports may be used for β-galactosidase immobilization as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation from the obtained reaction products is easy to accomplish.Programme Alβan, European Union Programme of High Level Scholarships for Latin AmericaBrazilian National Research Council (CNPq)Universidade do MinhoNeri, D. F. M.Balcão, V. M.Costa, Rafael S.Ferreira, Eugénio C.Torres, D.Rodrigues, Lígia M.Carvalho Junior, L. B.Teixeira, J. A.2008-092008-09-01T00:00:00Zconference paperinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/9268engFERREIRA, E. C. ; MOTA, M., ed. lit. – “Chempor 2008 : proceedings of the International Chemical and Biological Engineering Conference, 10, Braga, 2008”. Braga : Departamento de Engenharia Biológica da Universidade do Minho, 2008. ISBN 97 978-972-97810-3-2. p.1036-1041.978-972-97810-3-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:05:34Zoai:repositorium.sdum.uminho.pt:1822/9268Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:05:34Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
title |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
spellingShingle |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production Neri, D. F. M. β-galactosidase Immobilization Magnetic supports GOS Mathematical modeling |
title_short |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
title_full |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
title_fullStr |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
title_full_unstemmed |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
title_sort |
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production |
author |
Neri, D. F. M. |
author_facet |
Neri, D. F. M. Balcão, V. M. Costa, Rafael S. Ferreira, Eugénio C. Torres, D. Rodrigues, Lígia M. Carvalho Junior, L. B. Teixeira, J. A. |
author_role |
author |
author2 |
Balcão, V. M. Costa, Rafael S. Ferreira, Eugénio C. Torres, D. Rodrigues, Lígia M. Carvalho Junior, L. B. Teixeira, J. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Neri, D. F. M. Balcão, V. M. Costa, Rafael S. Ferreira, Eugénio C. Torres, D. Rodrigues, Lígia M. Carvalho Junior, L. B. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
β-galactosidase Immobilization Magnetic supports GOS Mathematical modeling |
topic |
β-galactosidase Immobilization Magnetic supports GOS Mathematical modeling |
description |
β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane- Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline (mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI) using glutaraldehyde as activating agent being the synthesis of GOS evaluated and compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by fitting the experimental data were compared in order to determine the effect of the immobilization process with different supports on the synthesis of oligosaccharides. These results clearly demonstrate that all supports may be used for β-galactosidase immobilization as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation from the obtained reaction products is easy to accomplish. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09 2008-09-01T00:00:00Z |
dc.type.driver.fl_str_mv |
conference paper |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/9268 |
url |
https://hdl.handle.net/1822/9268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FERREIRA, E. C. ; MOTA, M., ed. lit. – “Chempor 2008 : proceedings of the International Chemical and Biological Engineering Conference, 10, Braga, 2008”. Braga : Departamento de Engenharia Biológica da Universidade do Minho, 2008. ISBN 97 978-972-97810-3-2. p.1036-1041. 978-972-97810-3-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544512329744384 |