Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22617 |
Resumo: | This work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), were also determined. 7 The capacity of both strains to develop biofilms was greatly impaired in the 8 presence of CIP and GM. Sanitization was not complete allowing biofilm 9 recovery after the intermittent cycles of antibiotic pressure. The environmental 10 pressure exerted by CIP and GM did not develop P. aeruginosa resistance to 11 antibiotics nor cross-resistance towards BC. However, data highlighted that 12 none of the antimicrobials led to complete biofilm eradication, allowing the 13 recovery of the remaining adhered population possibly due to the selection of 14 persister cells. This feature may lead to biofilm recalcitrance, reinforce 15 bacterial attachment, and re-colonization of other sites. |
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Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosaClinically-relevant biofilmsThis work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), were also determined. 7 The capacity of both strains to develop biofilms was greatly impaired in the 8 presence of CIP and GM. Sanitization was not complete allowing biofilm 9 recovery after the intermittent cycles of antibiotic pressure. The environmental 10 pressure exerted by CIP and GM did not develop P. aeruginosa resistance to 11 antibiotics nor cross-resistance towards BC. However, data highlighted that 12 none of the antimicrobials led to complete biofilm eradication, allowing the 13 recovery of the remaining adhered population possibly due to the selection of 14 persister cells. This feature may lead to biofilm recalcitrance, reinforce 15 bacterial attachment, and re-colonization of other sites.Hindawi LimitedUniversidade do MinhoMachado, IdalinaGraça, JoanaLopes, H.Lopes, Susana PatríciaPereira, Maria Olívia20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22617engdalina Machado, Joana Graça, Hélder Lopes, Susana Lopes, and Maria O. Pereira, “Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa,” ISRN Biotechnology, vol. 2013, Article ID 178646, 10 pages, 2013. https://doi.org/10.5402/2013/178646.2090-940310.5402/2013/178646info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:32:20Zoai:repositorium.sdum.uminho.pt:1822/22617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:27:40.406927Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
title |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
spellingShingle |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa Machado, Idalina Clinically-relevant biofilms |
title_short |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
title_full |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
title_fullStr |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
title_full_unstemmed |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
title_sort |
Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa |
author |
Machado, Idalina |
author_facet |
Machado, Idalina Graça, Joana Lopes, H. Lopes, Susana Patrícia Pereira, Maria Olívia |
author_role |
author |
author2 |
Graça, Joana Lopes, H. Lopes, Susana Patrícia Pereira, Maria Olívia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Machado, Idalina Graça, Joana Lopes, H. Lopes, Susana Patrícia Pereira, Maria Olívia |
dc.subject.por.fl_str_mv |
Clinically-relevant biofilms |
topic |
Clinically-relevant biofilms |
description |
This work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), were also determined. 7 The capacity of both strains to develop biofilms was greatly impaired in the 8 presence of CIP and GM. Sanitization was not complete allowing biofilm 9 recovery after the intermittent cycles of antibiotic pressure. The environmental 10 pressure exerted by CIP and GM did not develop P. aeruginosa resistance to 11 antibiotics nor cross-resistance towards BC. However, data highlighted that 12 none of the antimicrobials led to complete biofilm eradication, allowing the 13 recovery of the remaining adhered population possibly due to the selection of 14 persister cells. This feature may lead to biofilm recalcitrance, reinforce 15 bacterial attachment, and re-colonization of other sites. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22617 |
url |
http://hdl.handle.net/1822/22617 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
dalina Machado, Joana Graça, Hélder Lopes, Susana Lopes, and Maria O. Pereira, “Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa,” ISRN Biotechnology, vol. 2013, Article ID 178646, 10 pages, 2013. https://doi.org/10.5402/2013/178646. 2090-9403 10.5402/2013/178646 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Limited |
publisher.none.fl_str_mv |
Hindawi Limited |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132769452818432 |