Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/110351 |
Resumo: | The hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NKT lymphocytes and myeloid cell, including the Mac-1+ (CD18+/CD11b+) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1β and increased TGF-β1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials. |
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Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regenerationAbsorbable ImplantsAnimalsBone Regeneration/drug effectsBone Regeneration/immunologyCytokines/immunologyDrug Implants/administration & dosageFemoral Fractures/immunologyFemoral Fractures/pathologyFemoral Fractures/therapyFibrinogen/administration & dosageFibrinogen/chemistryFracture Healing/drug effectsFracture Healing/immunologyGuided Tissue Regeneration/instrumentationImmunologic Factors/administration & dosageMaleRatsRats, WistarTissue ScaffoldsTreatment OutcomeThe hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NKT lymphocytes and myeloid cell, including the Mac-1+ (CD18+/CD11b+) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1β and increased TGF-β1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials.Elsevier20162016-01-01T00:00:00Z2018-12-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10216/110351eng0142-961210.1016/j.biomaterials.2016.10.004Vasconcelos, DMGonçalves, RMAlmeida, CRPereira, IOOliveira, MINeves, NSilva, AMRibeiro, ACCunha, CAlmeida, ARRibeiro, CCGil, AMSeebach, EKynast, KLRichter, WLamghari, MSantos, SGBarbosa, MAinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:32:20Zoai:repositorio-aberto.up.pt:10216/110351Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:26:01.822806Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
title |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
spellingShingle |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration Vasconcelos, DM Absorbable Implants Animals Bone Regeneration/drug effects Bone Regeneration/immunology Cytokines/immunology Drug Implants/administration & dosage Femoral Fractures/immunology Femoral Fractures/pathology Femoral Fractures/therapy Fibrinogen/administration & dosage Fibrinogen/chemistry Fracture Healing/drug effects Fracture Healing/immunology Guided Tissue Regeneration/instrumentation Immunologic Factors/administration & dosage Male Rats Rats, Wistar Tissue Scaffolds Treatment Outcome |
title_short |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
title_full |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
title_fullStr |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
title_full_unstemmed |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
title_sort |
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration |
author |
Vasconcelos, DM |
author_facet |
Vasconcelos, DM Gonçalves, RM Almeida, CR Pereira, IO Oliveira, MI Neves, N Silva, AM Ribeiro, AC Cunha, C Almeida, AR Ribeiro, CC Gil, AM Seebach, E Kynast, KL Richter, W Lamghari, M Santos, SG Barbosa, MA |
author_role |
author |
author2 |
Gonçalves, RM Almeida, CR Pereira, IO Oliveira, MI Neves, N Silva, AM Ribeiro, AC Cunha, C Almeida, AR Ribeiro, CC Gil, AM Seebach, E Kynast, KL Richter, W Lamghari, M Santos, SG Barbosa, MA |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vasconcelos, DM Gonçalves, RM Almeida, CR Pereira, IO Oliveira, MI Neves, N Silva, AM Ribeiro, AC Cunha, C Almeida, AR Ribeiro, CC Gil, AM Seebach, E Kynast, KL Richter, W Lamghari, M Santos, SG Barbosa, MA |
dc.subject.por.fl_str_mv |
Absorbable Implants Animals Bone Regeneration/drug effects Bone Regeneration/immunology Cytokines/immunology Drug Implants/administration & dosage Femoral Fractures/immunology Femoral Fractures/pathology Femoral Fractures/therapy Fibrinogen/administration & dosage Fibrinogen/chemistry Fracture Healing/drug effects Fracture Healing/immunology Guided Tissue Regeneration/instrumentation Immunologic Factors/administration & dosage Male Rats Rats, Wistar Tissue Scaffolds Treatment Outcome |
topic |
Absorbable Implants Animals Bone Regeneration/drug effects Bone Regeneration/immunology Cytokines/immunology Drug Implants/administration & dosage Femoral Fractures/immunology Femoral Fractures/pathology Femoral Fractures/therapy Fibrinogen/administration & dosage Fibrinogen/chemistry Fracture Healing/drug effects Fracture Healing/immunology Guided Tissue Regeneration/instrumentation Immunologic Factors/administration & dosage Male Rats Rats, Wistar Tissue Scaffolds Treatment Outcome |
description |
The hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NKT lymphocytes and myeloid cell, including the Mac-1+ (CD18+/CD11b+) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1β and increased TGF-β1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2018-12-31T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/110351 |
url |
http://hdl.handle.net/10216/110351 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0142-9612 10.1016/j.biomaterials.2016.10.004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136174136098816 |