Experimental determination of invasive fitness in Caenorhabditis elegans

Detalhes bibliográficos
Autor(a) principal: Chelo, Ivo M
Data de Publicação: 2014
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/809
Resumo: The deposited article is a post-print version and has been submitted to peer reviewing. This publication hasn't any creative commons license associated. The deposited article version contains attached some supplementary materials within the pdf. Some supplementary materials are not present in the uploaded version of the article, are present in the publisher's page in the following link: https://www.nature.com/articles/nprot.2014.098#integrated-supplementary-information
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spelling Experimental determination of invasive fitness in Caenorhabditis elegansAnimalsCaenorhabditis elegansComputer SimulationGenetic FitnessLikelihood FunctionsAllelesGenetics, PopulationThe deposited article is a post-print version and has been submitted to peer reviewing. This publication hasn't any creative commons license associated. The deposited article version contains attached some supplementary materials within the pdf. Some supplementary materials are not present in the uploaded version of the article, are present in the publisher's page in the following link: https://www.nature.com/articles/nprot.2014.098#integrated-supplementary-informationEstimation of fitness is a key step in experimental evolution studies. However, no established methods currently exist to specifically estimate how successful new alleles are in invading populations. The main reason is that most assays do not accurately reflect the randomness associated with the first stages of the invasion, when invaders are rare and extinctions are frequent. In this protocol, I describe how such experiments can be done in an effective way. By using the nematode model, Caenorhabditis elegans, a large number of invasion experiments are set up, whereby invading individuals carrying a visual marker are introduced into populations in very low numbers. The number of invaders counted in consecutive generations, together with the number of extinctions, is then used in the context of individual-based computer simulations to provide likelihood (Lk) estimates for fitness. This protocol can take up to five generations of experimental invasions and a few hours of computer processing time.Fundação para a Ciência e a Tecnologia grant: (EXPL/BIA-EFV/1211/2013); Human Frontiers Science Program grant: (RGP0045/2010).Nature Publishing GroupARCAChelo, Ivo M2017-11-22T12:34:53Z2014-05-222014-05-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/809engChelo, I. M. Experimental determination of invasive fitness in Caenorhabditis elegans. Nat Protoc 9, 1392–1400 (2014).10.1038/nprot.2014.098info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:13Zoai:arca.igc.gulbenkian.pt:10400.7/809Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:02.068373Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Experimental determination of invasive fitness in Caenorhabditis elegans
title Experimental determination of invasive fitness in Caenorhabditis elegans
spellingShingle Experimental determination of invasive fitness in Caenorhabditis elegans
Chelo, Ivo M
Animals
Caenorhabditis elegans
Computer Simulation
Genetic Fitness
Likelihood Functions
Alleles
Genetics, Population
title_short Experimental determination of invasive fitness in Caenorhabditis elegans
title_full Experimental determination of invasive fitness in Caenorhabditis elegans
title_fullStr Experimental determination of invasive fitness in Caenorhabditis elegans
title_full_unstemmed Experimental determination of invasive fitness in Caenorhabditis elegans
title_sort Experimental determination of invasive fitness in Caenorhabditis elegans
author Chelo, Ivo M
author_facet Chelo, Ivo M
author_role author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Chelo, Ivo M
dc.subject.por.fl_str_mv Animals
Caenorhabditis elegans
Computer Simulation
Genetic Fitness
Likelihood Functions
Alleles
Genetics, Population
topic Animals
Caenorhabditis elegans
Computer Simulation
Genetic Fitness
Likelihood Functions
Alleles
Genetics, Population
description The deposited article is a post-print version and has been submitted to peer reviewing. This publication hasn't any creative commons license associated. The deposited article version contains attached some supplementary materials within the pdf. Some supplementary materials are not present in the uploaded version of the article, are present in the publisher's page in the following link: https://www.nature.com/articles/nprot.2014.098#integrated-supplementary-information
publishDate 2014
dc.date.none.fl_str_mv 2014-05-22
2014-05-22T00:00:00Z
2017-11-22T12:34:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/809
url http://hdl.handle.net/10400.7/809
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chelo, I. M. Experimental determination of invasive fitness in Caenorhabditis elegans. Nat Protoc 9, 1392–1400 (2014).
10.1038/nprot.2014.098
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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