Variations on fibrinogen-erythrocyte interactions during cell aging

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2011
Outros Autores: Oliveira, Sofia de, Freitas, Teresa, Gonçalves, Sónia, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/6706
Resumo: © 2011 Carvalho et al
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spelling Variations on fibrinogen-erythrocyte interactions during cell aging© 2011 Carvalho et alErythrocyte hyperaggregation, a cardiovascular risk factor, is considered to be caused by an increase in plasma adhesion proteins, particularly fibrinogen. We have recently reported a specific binding between fibrinogen and an erythrocyte integrin receptor with a β3 or β3-like subunit. In this study we evaluate the influence of erythrocyte aging on the fibrinogen binding. By atomic force microscopy-based force spectroscopy measurements we found that increasing erythrocyte age, there is a decrease of the binding to fibrinogen by decreasing the frequency of its occurrence but not its force. This observation is reinforced by zeta-potential and fluorescence spectroscopy measurements. We conclude that upon erythrocyte aging the number of fibrinogen molecules bound to each cell decreases significantly, due to the progressive impairment of the specific fibrinogen-erythrocyte receptor interaction. Knowing that younger erythrocytes bind more to fibrinogen, we could presume that this population is the main contributor to the cardiovascular diseases associated with increased fibrinogen content in blood, which could disturb the blood flow. Our data also show that the sialic acids exposed on the erythrocyte membrane contribute for the interaction with fibrinogen, possibly by facilitating its binding to the erythrocyte membrane receptor.Fundação para a Ciência e a Tecnologia - Ministério da Ciência, Tecnologia e Ensino Superior and by Fundação Calouste Gulbenkian (Portugal).Public Library of ScienceRepositório da Universidade de LisboaCarvalho, Filomena A.Oliveira, Sofia deFreitas, TeresaGonçalves, SóniaSantos, Nuno C.2012-07-16T11:24:32Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6706engPLoS ONE 6(3): e181671932-6203http://dx.doi.org/10.1371/journal.pone.0018167info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:49:12Zoai:repositorio.ul.pt:10451/6706Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:40.927915Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Variations on fibrinogen-erythrocyte interactions during cell aging
title Variations on fibrinogen-erythrocyte interactions during cell aging
spellingShingle Variations on fibrinogen-erythrocyte interactions during cell aging
Carvalho, Filomena A.
title_short Variations on fibrinogen-erythrocyte interactions during cell aging
title_full Variations on fibrinogen-erythrocyte interactions during cell aging
title_fullStr Variations on fibrinogen-erythrocyte interactions during cell aging
title_full_unstemmed Variations on fibrinogen-erythrocyte interactions during cell aging
title_sort Variations on fibrinogen-erythrocyte interactions during cell aging
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Oliveira, Sofia de
Freitas, Teresa
Gonçalves, Sónia
Santos, Nuno C.
author_role author
author2 Oliveira, Sofia de
Freitas, Teresa
Gonçalves, Sónia
Santos, Nuno C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Oliveira, Sofia de
Freitas, Teresa
Gonçalves, Sónia
Santos, Nuno C.
description © 2011 Carvalho et al
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-07-16T11:24:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6706
url http://hdl.handle.net/10451/6706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE 6(3): e18167
1932-6203
http://dx.doi.org/10.1371/journal.pone.0018167
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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