Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/147022 |
Resumo: | The authors would like to thank the financial support of FEDER through the program COMPETE POCI-01-0145-FEDER-007440 and by Portuguese funds through FCT (UID/NEU/04539/2013 and PTDC/SAU-ENB/113696/2009 to R.P.N.), FCT (SFRH/BD/51942/2012 to A.L.); Bloodwise and CRUK program grants to T.E. BFU2013-45918-R and BFU2016-79127-R to F.J.I. |
| id |
RCAP_92c9419baf7b21d488d74109f01d03c2 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/147022 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulationGeneralThe authors would like to thank the financial support of FEDER through the program COMPETE POCI-01-0145-FEDER-007440 and by Portuguese funds through FCT (UID/NEU/04539/2013 and PTDC/SAU-ENB/113696/2009 to R.P.N.), FCT (SFRH/BD/51942/2012 to A.L.); Bloodwise and CRUK program grants to T.E. BFU2013-45918-R and BFU2016-79127-R to F.J.I.Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34 + umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.Faculdade de Ciências e Tecnologia (FCT)RUNLima, A. F.May, G.Colunga, J.Pedreiro, S.Paiva, A.Ferreira, L.Enver, T.Iborra, F. J.Pires Das Neves, R.2023-01-05T22:11:20Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147022eng2045-2322PURE: 13588502https://doi.org/10.1038/s41598-018-25517-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:00Zoai:run.unl.pt:10362/147022Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:45.036663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| title |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| spellingShingle |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation Lima, A. F. General |
| title_short |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| title_full |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| title_fullStr |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| title_full_unstemmed |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| title_sort |
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation |
| author |
Lima, A. F. |
| author_facet |
Lima, A. F. May, G. Colunga, J. Pedreiro, S. Paiva, A. Ferreira, L. Enver, T. Iborra, F. J. Pires Das Neves, R. |
| author_role |
author |
| author2 |
May, G. Colunga, J. Pedreiro, S. Paiva, A. Ferreira, L. Enver, T. Iborra, F. J. Pires Das Neves, R. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Faculdade de Ciências e Tecnologia (FCT) RUN |
| dc.contributor.author.fl_str_mv |
Lima, A. F. May, G. Colunga, J. Pedreiro, S. Paiva, A. Ferreira, L. Enver, T. Iborra, F. J. Pires Das Neves, R. |
| dc.subject.por.fl_str_mv |
General |
| topic |
General |
| description |
The authors would like to thank the financial support of FEDER through the program COMPETE POCI-01-0145-FEDER-007440 and by Portuguese funds through FCT (UID/NEU/04539/2013 and PTDC/SAU-ENB/113696/2009 to R.P.N.), FCT (SFRH/BD/51942/2012 to A.L.); Bloodwise and CRUK program grants to T.E. BFU2013-45918-R and BFU2016-79127-R to F.J.I. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12-01 2018-12-01T00:00:00Z 2023-01-05T22:11:20Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/147022 |
| url |
http://hdl.handle.net/10362/147022 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2045-2322 PURE: 13588502 https://doi.org/10.1038/s41598-018-25517-2 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138118409912320 |