Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/27334 https://doi.org/10.1016/j.idairyj.2013.05.019 |
Resumo: | A multi-residue screening method for 33 antibiotics from five different families was employed to simultaneously determine sulphonamide, tetracycline, macrolide, quinolone and chloramphenicol antibiotics using ultra high pressure liquid chromatography tandem mass spectrometry. A simple sample preparation method was developed using protein precipitation, centrifugation and solid phase extraction and was optimised to achieve the best recovery for all compounds. The methodology was validated for quantitative screening methods, by evaluating the detection capability (CCβ), specificity, selectivity, precision, applicability and ruggedness. Precision, in terms of relative standard deviation, was under 21% for all compounds. Because CCβ was determined for screening purposes and, according to maximum residue limit, the limit of detection of the method was calculated and ranged from 0.010 μg kg−1 to 3.7 μg kg−1. This validation provided evidence that the method is suitable to be applied in routine analysis for the detection of antibiotics in bovine, caprine and ovine milk. |
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Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milkA multi-residue screening method for 33 antibiotics from five different families was employed to simultaneously determine sulphonamide, tetracycline, macrolide, quinolone and chloramphenicol antibiotics using ultra high pressure liquid chromatography tandem mass spectrometry. A simple sample preparation method was developed using protein precipitation, centrifugation and solid phase extraction and was optimised to achieve the best recovery for all compounds. The methodology was validated for quantitative screening methods, by evaluating the detection capability (CCβ), specificity, selectivity, precision, applicability and ruggedness. Precision, in terms of relative standard deviation, was under 21% for all compounds. Because CCβ was determined for screening purposes and, according to maximum residue limit, the limit of detection of the method was calculated and ranged from 0.010 μg kg−1 to 3.7 μg kg−1. This validation provided evidence that the method is suitable to be applied in routine analysis for the detection of antibiotics in bovine, caprine and ovine milk.Elsevier2013-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/27334http://hdl.handle.net/10316/27334https://doi.org/10.1016/j.idairyj.2013.05.019engFREITAS, Andreia; BARBOSA, Jorge; RAMOS, Fernando - Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk. "International Dairy Journal". ISSN 0958-6946. Vol. 33 Nº. 1 (2013) p. 38-430958-6946http://www.sciencedirect.com/science/article/pii/S0958694613001520Freitas, AndreiaBarbosa, JorgeRamos, Fernandoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-05-25T07:46:04Zoai:estudogeral.uc.pt:10316/27334Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:49:59.145888Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
title |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
spellingShingle |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk Freitas, Andreia |
title_short |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
title_full |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
title_fullStr |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
title_full_unstemmed |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
title_sort |
Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk |
author |
Freitas, Andreia |
author_facet |
Freitas, Andreia Barbosa, Jorge Ramos, Fernando |
author_role |
author |
author2 |
Barbosa, Jorge Ramos, Fernando |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Freitas, Andreia Barbosa, Jorge Ramos, Fernando |
description |
A multi-residue screening method for 33 antibiotics from five different families was employed to simultaneously determine sulphonamide, tetracycline, macrolide, quinolone and chloramphenicol antibiotics using ultra high pressure liquid chromatography tandem mass spectrometry. A simple sample preparation method was developed using protein precipitation, centrifugation and solid phase extraction and was optimised to achieve the best recovery for all compounds. The methodology was validated for quantitative screening methods, by evaluating the detection capability (CCβ), specificity, selectivity, precision, applicability and ruggedness. Precision, in terms of relative standard deviation, was under 21% for all compounds. Because CCβ was determined for screening purposes and, according to maximum residue limit, the limit of detection of the method was calculated and ranged from 0.010 μg kg−1 to 3.7 μg kg−1. This validation provided evidence that the method is suitable to be applied in routine analysis for the detection of antibiotics in bovine, caprine and ovine milk. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/27334 http://hdl.handle.net/10316/27334 https://doi.org/10.1016/j.idairyj.2013.05.019 |
url |
http://hdl.handle.net/10316/27334 https://doi.org/10.1016/j.idairyj.2013.05.019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FREITAS, Andreia; BARBOSA, Jorge; RAMOS, Fernando - Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk. "International Dairy Journal". ISSN 0958-6946. Vol. 33 Nº. 1 (2013) p. 38-43 0958-6946 http://www.sciencedirect.com/science/article/pii/S0958694613001520 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133781010939904 |