Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/16000 |
Resumo: | The evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructured lipid carriers and polymeric nanoparticles. The effect of experimental conditions such as pH and the amount of surfactant present in the ultrafiltration media was addressed aiming at the selection of suitable conditions for the effective purification of nanocarriers. MTX-loaded nanoparticles were then submitted to ultrafiltration and the portions remaining in the upper compartment of the filtering device and in the ultrafiltrate were collected and analyzed by HPLC-UV using a reversed-phase (C18) monolithic column. A short centrifugation time (5 min) was suitable for establishing the amount of encapsulated MTX in nanostructured lipid carriers, based on the assumption that the free MTX concentration was the same in the upper compartment and in the ultrafiltrate. The defined conditions allowed the efficient separation of nanocarriers from the free drug, with recoveries of >85% even when nanoparticles were present in cell culture media and in pig skin surrogate from permeation assays. |
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Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in NanocarriersDrug deliveryUltrafiltrationEncapsulation efficiencySeparative methodsPermeationThe evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructured lipid carriers and polymeric nanoparticles. The effect of experimental conditions such as pH and the amount of surfactant present in the ultrafiltration media was addressed aiming at the selection of suitable conditions for the effective purification of nanocarriers. MTX-loaded nanoparticles were then submitted to ultrafiltration and the portions remaining in the upper compartment of the filtering device and in the ultrafiltrate were collected and analyzed by HPLC-UV using a reversed-phase (C18) monolithic column. A short centrifugation time (5 min) was suitable for establishing the amount of encapsulated MTX in nanostructured lipid carriers, based on the assumption that the free MTX concentration was the same in the upper compartment and in the ultrafiltrate. The defined conditions allowed the efficient separation of nanocarriers from the free drug, with recoveries of >85% even when nanoparticles were present in cell culture media and in pig skin surrogate from permeation assays.MDPIRepositório Científico do Instituto Politécnico do PortoMarques, Sara S.Ramos, Inês I.Fernandes, SaraBarreiros, LuisaLima, Sofia A. C.Reis, SaletteDomingues, M. Rosário M.Segundo, Marcela A.2020-06-19T09:28:14Z2020-042020-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/16000eng10.3390/molecules25081879info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:01:41Zoai:recipp.ipp.pt:10400.22/16000Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:35:44.370309Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
title |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
spellingShingle |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers Marques, Sara S. Drug delivery Ultrafiltration Encapsulation efficiency Separative methods Permeation |
title_short |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
title_full |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
title_fullStr |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
title_full_unstemmed |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
title_sort |
Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers |
author |
Marques, Sara S. |
author_facet |
Marques, Sara S. Ramos, Inês I. Fernandes, Sara Barreiros, Luisa Lima, Sofia A. C. Reis, Salette Domingues, M. Rosário M. Segundo, Marcela A. |
author_role |
author |
author2 |
Ramos, Inês I. Fernandes, Sara Barreiros, Luisa Lima, Sofia A. C. Reis, Salette Domingues, M. Rosário M. Segundo, Marcela A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Marques, Sara S. Ramos, Inês I. Fernandes, Sara Barreiros, Luisa Lima, Sofia A. C. Reis, Salette Domingues, M. Rosário M. Segundo, Marcela A. |
dc.subject.por.fl_str_mv |
Drug delivery Ultrafiltration Encapsulation efficiency Separative methods Permeation |
topic |
Drug delivery Ultrafiltration Encapsulation efficiency Separative methods Permeation |
description |
The evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructured lipid carriers and polymeric nanoparticles. The effect of experimental conditions such as pH and the amount of surfactant present in the ultrafiltration media was addressed aiming at the selection of suitable conditions for the effective purification of nanocarriers. MTX-loaded nanoparticles were then submitted to ultrafiltration and the portions remaining in the upper compartment of the filtering device and in the ultrafiltrate were collected and analyzed by HPLC-UV using a reversed-phase (C18) monolithic column. A short centrifugation time (5 min) was suitable for establishing the amount of encapsulated MTX in nanostructured lipid carriers, based on the assumption that the free MTX concentration was the same in the upper compartment and in the ultrafiltrate. The defined conditions allowed the efficient separation of nanocarriers from the free drug, with recoveries of >85% even when nanoparticles were present in cell culture media and in pig skin surrogate from permeation assays. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-19T09:28:14Z 2020-04 2020-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/16000 |
url |
http://hdl.handle.net/10400.22/16000 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/molecules25081879 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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