Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis

Detalhes bibliográficos
Autor(a) principal: Tuna, Stepan
Data de Publicação: 2020
Outros Autores: Izaguirre, Jon, Bondar, Marina, Marques, Mafalda, Fernandes, Pedro Carlos de Barros, Fonseca, Maria Manuela Regalo da, Cesário, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://www.sciencedirect.com/science/article/pii/S2215017X20301892#!
http://hdl.handle.net/10437/10408
Resumo: Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates.
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spelling Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensisBIOTECNOLOGIAGELIDIUM SESQUIPEDALEHALOMONAS BOLIVIENSISRESÍDUOS DE ALGAS MARINHASPOLI-3-HIDROXIBUTIRATORESÍDUOS DE MACROALGASENGENHARIA BIOTECNOLÓGICAALGASBIOTECHNOLOGYGELIDIUM SESQUIPEDALEHALOMONAS BOLIVIENSISSEAWEED RESIDUESPOLY-3-HYDROXYBUTYRATEMACROALGAE RESIDUESBIOTECHNOLOGY ENGINEERINGALGAEAgar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates.Elsevier2020-08-31T11:05:34Z2020-09-01T00:00:00Z2020-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S2215017X20301892#!http://hdl.handle.net/10437/10408engTuna, StepanIzaguirre, JonBondar, MarinaMarques, MafaldaFernandes, Pedro Carlos de BarrosFonseca, Maria Manuela Regalo daCesário, Mariainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:05:13Zoai:recil.ensinolusofona.pt:10437/10408Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:13:01.262458Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
title Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
spellingShingle Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
Tuna, Stepan
BIOTECNOLOGIA
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
RESÍDUOS DE ALGAS MARINHAS
POLI-3-HIDROXIBUTIRATO
RESÍDUOS DE MACROALGAS
ENGENHARIA BIOTECNOLÓGICA
ALGAS
BIOTECHNOLOGY
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
SEAWEED RESIDUES
POLY-3-HYDROXYBUTYRATE
MACROALGAE RESIDUES
BIOTECHNOLOGY ENGINEERING
ALGAE
title_short Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
title_full Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
title_fullStr Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
title_full_unstemmed Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
title_sort Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis
author Tuna, Stepan
author_facet Tuna, Stepan
Izaguirre, Jon
Bondar, Marina
Marques, Mafalda
Fernandes, Pedro Carlos de Barros
Fonseca, Maria Manuela Regalo da
Cesário, Maria
author_role author
author2 Izaguirre, Jon
Bondar, Marina
Marques, Mafalda
Fernandes, Pedro Carlos de Barros
Fonseca, Maria Manuela Regalo da
Cesário, Maria
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tuna, Stepan
Izaguirre, Jon
Bondar, Marina
Marques, Mafalda
Fernandes, Pedro Carlos de Barros
Fonseca, Maria Manuela Regalo da
Cesário, Maria
dc.subject.por.fl_str_mv BIOTECNOLOGIA
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
RESÍDUOS DE ALGAS MARINHAS
POLI-3-HIDROXIBUTIRATO
RESÍDUOS DE MACROALGAS
ENGENHARIA BIOTECNOLÓGICA
ALGAS
BIOTECHNOLOGY
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
SEAWEED RESIDUES
POLY-3-HYDROXYBUTYRATE
MACROALGAE RESIDUES
BIOTECHNOLOGY ENGINEERING
ALGAE
topic BIOTECNOLOGIA
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
RESÍDUOS DE ALGAS MARINHAS
POLI-3-HIDROXIBUTIRATO
RESÍDUOS DE MACROALGAS
ENGENHARIA BIOTECNOLÓGICA
ALGAS
BIOTECHNOLOGY
GELIDIUM SESQUIPEDALE
HALOMONAS BOLIVIENSIS
SEAWEED RESIDUES
POLY-3-HYDROXYBUTYRATE
MACROALGAE RESIDUES
BIOTECHNOLOGY ENGINEERING
ALGAE
description Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-31T11:05:34Z
2020-09-01T00:00:00Z
2020-09
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.sciencedirect.com/science/article/pii/S2215017X20301892#!
http://hdl.handle.net/10437/10408
url https://www.sciencedirect.com/science/article/pii/S2215017X20301892#!
http://hdl.handle.net/10437/10408
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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