"Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings

Detalhes bibliográficos
Autor(a) principal: Martins, A. M.
Data de Publicação: 2008
Outros Autores: Pham, Q. P., Malafaya, P. B., Raphael, R. M., Kasper, F. K., Reis, R. L., Mikos, A. G.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59133
Resumo: [Excerpt] The present study reports the use of non-porous, ‘‘smart’’ and stimulus responsive chitosan-based scaffolds with the capability of gradual in situ pore formation for bone tissue engineering applications. Biomimetic calcium phosphate (CaP) coatings were used as a strategy to incorporate lysozyme at the surface of chitosan based materials the main objective of controlling their degradation profile as a function of immersion time. In order to confirm the concept, degradation tests with concentration similar to those incorporated into CaP chitosan-based scaffolds were used to study the degradation of the scaffolds and the formation of pores as function of immersion time. Degradation studies with lysozyme (1.5 g/L) showed the formation of pores, indicating an increase of porosity (~5% - 55% up to 21 days) resulting in porous 3-D structures with interconnected pores. […]
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spelling "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatingsScience & Technology[Excerpt] The present study reports the use of non-porous, ‘‘smart’’ and stimulus responsive chitosan-based scaffolds with the capability of gradual in situ pore formation for bone tissue engineering applications. Biomimetic calcium phosphate (CaP) coatings were used as a strategy to incorporate lysozyme at the surface of chitosan based materials the main objective of controlling their degradation profile as a function of immersion time. In order to confirm the concept, degradation tests with concentration similar to those incorporated into CaP chitosan-based scaffolds were used to study the degradation of the scaffolds and the formation of pores as function of immersion time. Degradation studies with lysozyme (1.5 g/L) showed the formation of pores, indicating an increase of porosity (~5% - 55% up to 21 days) resulting in porous 3-D structures with interconnected pores. […]info:eu-repo/semantics/publishedVersionMary Ann Liebert Inc.Universidade do MinhoMartins, A. M.Pham, Q. P.Malafaya, P. B.Raphael, R. M.Kasper, F. K.Reis, R. L.Mikos, A. G.20082008-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/59133eng1937-3341info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:16:17Zoai:repositorium.sdum.uminho.pt:1822/59133Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:16:17Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
title "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
spellingShingle "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
Martins, A. M.
Science & Technology
title_short "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
title_full "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
title_fullStr "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
title_full_unstemmed "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
title_sort "Smart'' and stimulus responsive chitosan-based scaffolds/cells for bone tissue engineering: Influence of lysozyme upon scaffold degradation and osteogenic differentiation of cultured marrow stromal cells induced by cap coatings
author Martins, A. M.
author_facet Martins, A. M.
Pham, Q. P.
Malafaya, P. B.
Raphael, R. M.
Kasper, F. K.
Reis, R. L.
Mikos, A. G.
author_role author
author2 Pham, Q. P.
Malafaya, P. B.
Raphael, R. M.
Kasper, F. K.
Reis, R. L.
Mikos, A. G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, A. M.
Pham, Q. P.
Malafaya, P. B.
Raphael, R. M.
Kasper, F. K.
Reis, R. L.
Mikos, A. G.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description [Excerpt] The present study reports the use of non-porous, ‘‘smart’’ and stimulus responsive chitosan-based scaffolds with the capability of gradual in situ pore formation for bone tissue engineering applications. Biomimetic calcium phosphate (CaP) coatings were used as a strategy to incorporate lysozyme at the surface of chitosan based materials the main objective of controlling their degradation profile as a function of immersion time. In order to confirm the concept, degradation tests with concentration similar to those incorporated into CaP chitosan-based scaffolds were used to study the degradation of the scaffolds and the formation of pores as function of immersion time. Degradation studies with lysozyme (1.5 g/L) showed the formation of pores, indicating an increase of porosity (~5% - 55% up to 21 days) resulting in porous 3-D structures with interconnected pores. […]
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59133
url http://hdl.handle.net/1822/59133
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1937-3341
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc.
publisher.none.fl_str_mv Mary Ann Liebert Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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