Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/37631 |
Resumo: | Polymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules. |
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Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiencyDoxorubicinPolymeric nanoparticleSN-38Surfactant-drug interactionPolymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules.Veritati - Repositório Institucional da Universidade Católica PortuguesaRebanda, Magda M.Bettini, SimonaBlasi, LauraGaballo, AntonioRagusa, AndreaQuarta, AlessandraPiccirillo, Clara2022-05-18T15:58:22Z2022-05-012022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/37631eng2079-499110.3390/nano1209155085129125792PMC910393535564258000795341900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-16T01:43:45Zoai:repositorio.ucp.pt:10400.14/37631Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:30:39.260076Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
title |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
spellingShingle |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency Rebanda, Magda M. Doxorubicin Polymeric nanoparticle SN-38 Surfactant-drug interaction |
title_short |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
title_full |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
title_fullStr |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
title_full_unstemmed |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
title_sort |
Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency |
author |
Rebanda, Magda M. |
author_facet |
Rebanda, Magda M. Bettini, Simona Blasi, Laura Gaballo, Antonio Ragusa, Andrea Quarta, Alessandra Piccirillo, Clara |
author_role |
author |
author2 |
Bettini, Simona Blasi, Laura Gaballo, Antonio Ragusa, Andrea Quarta, Alessandra Piccirillo, Clara |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Rebanda, Magda M. Bettini, Simona Blasi, Laura Gaballo, Antonio Ragusa, Andrea Quarta, Alessandra Piccirillo, Clara |
dc.subject.por.fl_str_mv |
Doxorubicin Polymeric nanoparticle SN-38 Surfactant-drug interaction |
topic |
Doxorubicin Polymeric nanoparticle SN-38 Surfactant-drug interaction |
description |
Polymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-18T15:58:22Z 2022-05-01 2022-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/37631 |
url |
http://hdl.handle.net/10400.14/37631 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2079-4991 10.3390/nano12091550 85129125792 PMC9103935 35564258 000795341900001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132029040721920 |