Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens

Detalhes bibliográficos
Autor(a) principal: Zwar, Ingrid P.
Data de Publicação: 2023
Outros Autores: Trotta, Caterina do Valle, Ziotti, Ana B. S., Neto, Milton Lima, Araújo, Welington L., Melo, Itamar S. de, Ottoni, Cristiane A., Souza, Ana O. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/29060
Resumo: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, inthis study, two strains of actinomycetes isolated from the soil of the Brazilianbiome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) wereutilized. The strains were identified by using the 16S rRNA gene sequencing asStreptomycessp., related toStreptomyces mimosusspecies. The obtained AgNPswere coded as AgNPs35and AgNPs58and characterized by size andmorphology using dynamic light scattering, zeta potential, transmissionelectron microscopy, and Fourier transformed infrared (FTIR). The antifungalactivity of the AgNPs35and AgNPs58was evaluatedin vitroby the minimalinhibitory concentration (MIC) assay on the phytopathogens,Alternariasolani,Alternaria alternata, andColletotrichum gloeosporioides. The phytotoxiceffect was evaluated by the germination rate and seedling growth of rice(Oryza sativa). AgNPs35and AgNPs58showed surface plasmon resonance andaverage sizes of 30 and 60 nm, respectively. Both AgNPs presented sphericalshape and the FTIR analysis confirmed the presence of functional groups suchas free amines and hydroxyls of biomolecules bounded to the external layer ofthe nanoparticles. Both AgNPs inhibited the growth of the three phytopatho-gens tested, andA. alternatewas the most sensible (MIC≤4 μM). Moreover,the AgNPs35and AgNPs58did not induce phytotoxic effects on thegermination and development of rice seedlings. In conclusion, these AgNPsare promising candidates to biocontrol of these phytopathogens withoutendangering rice plants
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spelling Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogensactinomycetesbiocontrolbiogenic silver nanoparticlesphytotoxicityrice (Oryza sativa)To find effective silver nanoparticles (AgNPs) for control of phytopathogens, inthis study, two strains of actinomycetes isolated from the soil of the Brazilianbiome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) wereutilized. The strains were identified by using the 16S rRNA gene sequencing asStreptomycessp., related toStreptomyces mimosusspecies. The obtained AgNPswere coded as AgNPs35and AgNPs58and characterized by size andmorphology using dynamic light scattering, zeta potential, transmissionelectron microscopy, and Fourier transformed infrared (FTIR). The antifungalactivity of the AgNPs35and AgNPs58was evaluatedin vitroby the minimalinhibitory concentration (MIC) assay on the phytopathogens,Alternariasolani,Alternaria alternata, andColletotrichum gloeosporioides. The phytotoxiceffect was evaluated by the germination rate and seedling growth of rice(Oryza sativa). AgNPs35and AgNPs58showed surface plasmon resonance andaverage sizes of 30 and 60 nm, respectively. Both AgNPs presented sphericalshape and the FTIR analysis confirmed the presence of functional groups suchas free amines and hydroxyls of biomolecules bounded to the external layer ofthe nanoparticles. Both AgNPs inhibited the growth of the three phytopatho-gens tested, andA. alternatewas the most sensible (MIC≤4 μM). Moreover,the AgNPs35and AgNPs58did not induce phytotoxic effects on thegermination and development of rice seedlings. In conclusion, these AgNPsare promising candidates to biocontrol of these phytopathogens withoutendangering rice plantsWileyRepositório da Universidade de LisboaZwar, Ingrid P.Trotta, Caterina do ValleZiotti, Ana B. S.Neto, Milton LimaAraújo, Welington L.Melo, Itamar S. deOttoni, Cristiane A.Souza, Ana O. de2023-10-17T15:39:05Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/29060engZwar, IP, Trotta, CdV, Ziotti, ABS, Lima Neto, M, Araújo, WL, de Melo, IS, et al. Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens. J Basic Microbiol. 2023; 63: 64–74.10.1002/jobm.202200439metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-22T01:32:39Zoai:www.repository.utl.pt:10400.5/29060Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:39:20.796370Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
title Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
spellingShingle Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
Zwar, Ingrid P.
actinomycetes
biocontrol
biogenic silver nanoparticles
phytotoxicity
rice (Oryza sativa)
title_short Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
title_full Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
title_fullStr Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
title_full_unstemmed Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
title_sort Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
author Zwar, Ingrid P.
author_facet Zwar, Ingrid P.
Trotta, Caterina do Valle
Ziotti, Ana B. S.
Neto, Milton Lima
Araújo, Welington L.
Melo, Itamar S. de
Ottoni, Cristiane A.
Souza, Ana O. de
author_role author
author2 Trotta, Caterina do Valle
Ziotti, Ana B. S.
Neto, Milton Lima
Araújo, Welington L.
Melo, Itamar S. de
Ottoni, Cristiane A.
Souza, Ana O. de
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Zwar, Ingrid P.
Trotta, Caterina do Valle
Ziotti, Ana B. S.
Neto, Milton Lima
Araújo, Welington L.
Melo, Itamar S. de
Ottoni, Cristiane A.
Souza, Ana O. de
dc.subject.por.fl_str_mv actinomycetes
biocontrol
biogenic silver nanoparticles
phytotoxicity
rice (Oryza sativa)
topic actinomycetes
biocontrol
biogenic silver nanoparticles
phytotoxicity
rice (Oryza sativa)
description To find effective silver nanoparticles (AgNPs) for control of phytopathogens, inthis study, two strains of actinomycetes isolated from the soil of the Brazilianbiome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) wereutilized. The strains were identified by using the 16S rRNA gene sequencing asStreptomycessp., related toStreptomyces mimosusspecies. The obtained AgNPswere coded as AgNPs35and AgNPs58and characterized by size andmorphology using dynamic light scattering, zeta potential, transmissionelectron microscopy, and Fourier transformed infrared (FTIR). The antifungalactivity of the AgNPs35and AgNPs58was evaluatedin vitroby the minimalinhibitory concentration (MIC) assay on the phytopathogens,Alternariasolani,Alternaria alternata, andColletotrichum gloeosporioides. The phytotoxiceffect was evaluated by the germination rate and seedling growth of rice(Oryza sativa). AgNPs35and AgNPs58showed surface plasmon resonance andaverage sizes of 30 and 60 nm, respectively. Both AgNPs presented sphericalshape and the FTIR analysis confirmed the presence of functional groups suchas free amines and hydroxyls of biomolecules bounded to the external layer ofthe nanoparticles. Both AgNPs inhibited the growth of the three phytopatho-gens tested, andA. alternatewas the most sensible (MIC≤4 μM). Moreover,the AgNPs35and AgNPs58did not induce phytotoxic effects on thegermination and development of rice seedlings. In conclusion, these AgNPsare promising candidates to biocontrol of these phytopathogens withoutendangering rice plants
publishDate 2023
dc.date.none.fl_str_mv 2023-10-17T15:39:05Z
2023-01
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/29060
url http://hdl.handle.net/10400.5/29060
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zwar, IP, Trotta, CdV, Ziotti, ABS, Lima Neto, M, Araújo, WL, de Melo, IS, et al. Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens. J Basic Microbiol. 2023; 63: 64–74.
10.1002/jobm.202200439
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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