Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/2521 |
Resumo: | Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC - 32, Control - 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log2 fold change (log2 FC) value of ≥0.58/≤-0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future. |
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Volatilomic insight of head and neck cancer via the effects observed on saliva metabolitesDiagnostic markersMass spectrometryMetabolomicsOral cancer detection.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da MadeiraHead and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC - 32, Control - 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log2 fold change (log2 FC) value of ≥0.58/≤-0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future.Nature ResearchDigitUMaTaware, RavindraTaunk, KhushmanPereira, Jorge A. M.Shirolkar, AmeySoneji, DharmeshCâmara, José S.Nagarajaram, H. A.Rapole, Srikanth2019-07-30T08:54:28Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/2521engTaware, R., Taunk, K., Pereira, J. A., Shirolkar, A., Soneji, D., Câmara, J. S., ... & Rapole, S. (2018). Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites. Scientific reports, 8(1), 17725.2045-232210.1038/s41598-018-35854-xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-17T05:57:35Zoai:digituma.uma.pt:10400.13/2521Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:05:18.611794Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
title |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
spellingShingle |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites Taware, Ravindra Diagnostic markers Mass spectrometry Metabolomics Oral cancer detection . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
title_short |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
title_full |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
title_fullStr |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
title_full_unstemmed |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
title_sort |
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites |
author |
Taware, Ravindra |
author_facet |
Taware, Ravindra Taunk, Khushman Pereira, Jorge A. M. Shirolkar, Amey Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
author_role |
author |
author2 |
Taunk, Khushman Pereira, Jorge A. M. Shirolkar, Amey Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Taware, Ravindra Taunk, Khushman Pereira, Jorge A. M. Shirolkar, Amey Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
dc.subject.por.fl_str_mv |
Diagnostic markers Mass spectrometry Metabolomics Oral cancer detection . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
topic |
Diagnostic markers Mass spectrometry Metabolomics Oral cancer detection . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
description |
Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC - 32, Control - 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log2 fold change (log2 FC) value of ≥0.58/≤-0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-07-30T08:54:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/2521 |
url |
http://hdl.handle.net/10400.13/2521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Taware, R., Taunk, K., Pereira, J. A., Shirolkar, A., Soneji, D., Câmara, J. S., ... & Rapole, S. (2018). Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites. Scientific reports, 8(1), 17725. 2045-2322 10.1038/s41598-018-35854-x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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