Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1038/s41598-019-49195-w |
Resumo: | We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029). |
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Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinomaGeneralSDG 3 - Good Health and Well-beingWe would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029).The lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing approach of 16S ribosomal RNA gene (V3-V6) using a total of 103 bronchoalveaolar lavage fluid samples. Then, identified taxa were used to inspect 1009 cases from The Cancer Genome Atlas and to annotate tumor unmapped RNAseq reads. Microbial diversity was analyzed per cancer subtype, history of cigarette smoking and airflow obstruction, among other clinical data. We show that LC microbiota is enriched in Proteobacteria and more diverse in SCC than ADC, particularly in males and heavier smokers. High frequencies of Proteobacteria were found to discriminate a major cluster, further subdivided into well-defined communities’ associated with either ADC or SCC. Here, a SCC subcluster differing from other cases by a worse survival was correlated with several Enterobacteriaceae. Overall, this study provides first evidence for a correlation between lung microbiota and cancer subtype and for its influence on patient life expectancy.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNGomes, SílviaCavadas, BrunoFerreira, Joana CatarinaMarques, Patrícia IsabelMonteiro, CatarinaSucena, MariaSousa, CatarinaVaz Rodrigues, LuísTeixeira, GilbertoPinto, PaulaTavares de Abreu, TiagoBárbara, CristinaSemedo, JúlioMota, LeonorCarvalho, Ana SofiaMatthiesen, RunePereira, LuísaSeixas, Susana2019-09-20T22:42:11Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-019-49195-weng2045-2322PURE: 14750540http://www.scopus.com/inward/record.url?scp=85071896383&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-019-49195-winfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:36:27Zoai:run.unl.pt:10362/81887Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:09.069078Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
title |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
spellingShingle |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma Gomes, Sílvia General SDG 3 - Good Health and Well-being |
title_short |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
title_full |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
title_fullStr |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
title_full_unstemmed |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
title_sort |
Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma |
author |
Gomes, Sílvia |
author_facet |
Gomes, Sílvia Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
author_role |
author |
author2 |
Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
dc.contributor.author.fl_str_mv |
Gomes, Sílvia Cavadas, Bruno Ferreira, Joana Catarina Marques, Patrícia Isabel Monteiro, Catarina Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Pereira, Luísa Seixas, Susana |
dc.subject.por.fl_str_mv |
General SDG 3 - Good Health and Well-being |
topic |
General SDG 3 - Good Health and Well-being |
description |
We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON. 2 - O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referencia Estrategico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-20T22:42:11Z 2019-12-01 2019-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1038/s41598-019-49195-w |
url |
https://doi.org/10.1038/s41598-019-49195-w |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 PURE: 14750540 http://www.scopus.com/inward/record.url?scp=85071896383&partnerID=8YFLogxK https://doi.org/10.1038/s41598-019-49195-w |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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