The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1319 |
Resumo: | Aims: To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum. Methods and results: Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 ºC, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2–3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used. Conclusions: Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ. Significance and Impact of the Study: Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used. |
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The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum LinkScience & TechnologyAims: To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum. Methods and results: Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 ºC, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2–3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used. Conclusions: Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ. Significance and Impact of the Study: Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used.The Society for Applied MicrobiologyUniversidade do MinhoSantos, Isabel M.Abrunhosa, LuísVenâncio, ArmandoLima, Nelson20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1319eng"Letters in applied microbiology". ISSN 0266-8254. 35:4 (2002) 272-275.0266-825410.1046/j.1472-765X.2002.01186.x12358686info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:10Zoai:repositorium.sdum.uminho.pt:1822/1319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:15.288215Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
title |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
spellingShingle |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link Santos, Isabel M. Science & Technology |
title_short |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
title_full |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
title_fullStr |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
title_full_unstemmed |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
title_sort |
The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link |
author |
Santos, Isabel M. |
author_facet |
Santos, Isabel M. Abrunhosa, Luís Venâncio, Armando Lima, Nelson |
author_role |
author |
author2 |
Abrunhosa, Luís Venâncio, Armando Lima, Nelson |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Isabel M. Abrunhosa, Luís Venâncio, Armando Lima, Nelson |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Aims: To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum. Methods and results: Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 ºC, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2–3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used. Conclusions: Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ. Significance and Impact of the Study: Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 2002-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1319 |
url |
http://hdl.handle.net/1822/1319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Letters in applied microbiology". ISSN 0266-8254. 35:4 (2002) 272-275. 0266-8254 10.1046/j.1472-765X.2002.01186.x 12358686 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Society for Applied Microbiology |
publisher.none.fl_str_mv |
The Society for Applied Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132570578845696 |