Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/49032 |
Resumo: | Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1 and testosterone levels were evaluated by ELISA. Penile SDF-1 protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1 levels, which were significantly decreased in animals with diabetes. SDF-1 protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1 protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways. |
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Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?Erectile DysfunctionDiabetesEndothelial Progenitor CellsInsulinStromal Derived Factor-1 AlphaCiências Médicas::Medicina BásicaScience & TechnologyErectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1 and testosterone levels were evaluated by ELISA. Penile SDF-1 protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1 levels, which were significantly decreased in animals with diabetes. SDF-1 protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1 protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways.European Society for Sexual Medicine (ESSM Award for Medical Research 2011), and by the Portuguese Society of Andrology (Professor Alexandre Moreira Award “Research in Sexual Medicine 2013”)info:eu-repo/semantics/publishedVersionWileyUniversidade do MinhoCastela, AngelaGomes, PedroSilvestre, Ricardo Jorge LealGuardão, LuísaLeite, LilianaChilro, RuiRodrigues, IldaVendeira, PedroVirag, RonaldCosta, Carla20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/49032engCastela, A., Gomes, P., Silvestre, R., Guardão, L., Leite, L., Chilro, R., ... & Costa, C. (2017). Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?. Journal of cellular biochemistry, 118(1), 82-911097-464410.1002/jcb.2561327237706http://onlinelibrary.wiley.com/doi/10.1002/jcb.25613/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:09Zoai:repositorium.sdum.uminho.pt:1822/49032Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:19.973672Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
title |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
spellingShingle |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? Castela, Angela Erectile Dysfunction Diabetes Endothelial Progenitor Cells Insulin Stromal Derived Factor-1 Alpha Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
title_full |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
title_fullStr |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
title_full_unstemmed |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
title_sort |
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control? |
author |
Castela, Angela |
author_facet |
Castela, Angela Gomes, Pedro Silvestre, Ricardo Jorge Leal Guardão, Luísa Leite, Liliana Chilro, Rui Rodrigues, Ilda Vendeira, Pedro Virag, Ronald Costa, Carla |
author_role |
author |
author2 |
Gomes, Pedro Silvestre, Ricardo Jorge Leal Guardão, Luísa Leite, Liliana Chilro, Rui Rodrigues, Ilda Vendeira, Pedro Virag, Ronald Costa, Carla |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Castela, Angela Gomes, Pedro Silvestre, Ricardo Jorge Leal Guardão, Luísa Leite, Liliana Chilro, Rui Rodrigues, Ilda Vendeira, Pedro Virag, Ronald Costa, Carla |
dc.subject.por.fl_str_mv |
Erectile Dysfunction Diabetes Endothelial Progenitor Cells Insulin Stromal Derived Factor-1 Alpha Ciências Médicas::Medicina Básica Science & Technology |
topic |
Erectile Dysfunction Diabetes Endothelial Progenitor Cells Insulin Stromal Derived Factor-1 Alpha Ciências Médicas::Medicina Básica Science & Technology |
description |
Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1 and testosterone levels were evaluated by ELISA. Penile SDF-1 protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1 levels, which were significantly decreased in animals with diabetes. SDF-1 protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1 protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/49032 |
url |
http://hdl.handle.net/1822/49032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castela, A., Gomes, P., Silvestre, R., Guardão, L., Leite, L., Chilro, R., ... & Costa, C. (2017). Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?. Journal of cellular biochemistry, 118(1), 82-91 1097-4644 10.1002/jcb.25613 27237706 http://onlinelibrary.wiley.com/doi/10.1002/jcb.25613/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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