Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals

Detalhes bibliográficos
Autor(a) principal: Silva, Nadia
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/799
Resumo: Dissertação mest., Ciências Biomédicas, Universidade do Algarve, 2009
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spelling Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary mineralsTesesTrutaOncorhynchus mykissCálcioFósforoEsqueletoGenéticaDissertação mest., Ciências Biomédicas, Universidade do Algarve, 2009Calcium (Ca) and phosphorus (P) play a critical role in diverse biological processes, and their regulation is essential for organism health. In the present study, the adaptive response to Ca and P dietary availability in trout (Oncorhynchus mykiss) was evaluated taking an integrative approach. The impact of dietary restriction in Ca and P on skeletal ontogeny, morphology and density was assessed. The expression of previously known Pi responsive genes, along with calciotropic hormones STC, CT and extracellular matrix proteins (osteonectin and osteopontin) was evaluated by RT-qPCR. The activity of the ultimobrachial gland and corpuscles of Stannius was determined by morphometry after immunohistochemistry for calcitonin (CT) and stanniocalcin (STC) respectively. P restricted individuals showed decreased whole body P and Ca content throughout the experiment. The restriction of dietary P led to delayed skeletal ontogeny which affected the mineralization level of both endochondral and dermal bones, and was associated with an increase in the incidence of skeletal malformation. Low Ca and increased P in the diet also delayed skeletal development at D11 but this effect was transient. High dietary P caused increased plasma levels of P by D11 and a cumulative mortality rate of 90% by the end of the experiment. These individuals had denser bones and a lower incidence of malformations, possibly due to the effect of “survival of the fittest”. Of the previously identified molecular markers of P deficiency in trout S10011A was found to be significantly downregulated in P deficient trout fry, while INaPiIIb was dowregulated in trout fry on low Ca and high P diets. The calciotropic hormone STC was upregulated in trout fry on low P diets and CT was downregulated in trout fry on high P and low Ca diets. OSN expression was also downregulated in the high P group. The study indicates that modifications in the dietary Ca and P during development significantly modify the ontogeny and density of the skeleton. The availability of Ca and P also led to modification in endocrine factors presumably to ensure maintenance of mineral homeostasis. It seems likely that changes in hormone production may be responsible for changes in transcript abundance of specific transporters/binding proteins of Ca and P although in the present study it was not possible to test this hypothesis.Power, DeborahSapientiaSilva, Nadia2011-09-07T16:05:37Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.1/799por597 SIL*Mor Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:38Zoai:sapientia.ualg.pt:10400.1/799Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:05.836444Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
title Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
spellingShingle Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
Silva, Nadia
Teses
Truta
Oncorhynchus mykiss
Cálcio
Fósforo
Esqueleto
Genética
title_short Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
title_full Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
title_fullStr Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
title_full_unstemmed Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
title_sort Morphologic and molecular responses of developing rainbow trout (Oncorhynchus mykiss) to dietary minerals
author Silva, Nadia
author_facet Silva, Nadia
author_role author
dc.contributor.none.fl_str_mv Power, Deborah
Sapientia
dc.contributor.author.fl_str_mv Silva, Nadia
dc.subject.por.fl_str_mv Teses
Truta
Oncorhynchus mykiss
Cálcio
Fósforo
Esqueleto
Genética
topic Teses
Truta
Oncorhynchus mykiss
Cálcio
Fósforo
Esqueleto
Genética
description Dissertação mest., Ciências Biomédicas, Universidade do Algarve, 2009
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2011-09-07T16:05:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/799
url http://hdl.handle.net/10400.1/799
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 597 SIL*Mor Cave
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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