Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31860 |
Resumo: | One of the most important targets and challenges in the second generation bioethanol is the development of a cost-effective process on large-scale. In this context, the high solid loading on saccharification and fermentation and the use of whole-slurry from pretreatment could be promising alternatives to obtain high ethanol concentrations and to decrease operational costs and wastewater. In this work, Eucalyptus globulus wood was submitted to non-isothermal autohydrolysis treatment (Tmax = 210 °C) and the whole-slurry obtained was assayed for the optimization of enzymatic saccharification at different solid and enzymes (CTec2 and HTec2) loadings using a Box–Behnken experimental design. Under the optimized conditions (liquid solid ratio 6.4 g/g, cellulase to substrate ratio 22.5 FPU/g and hemicellulase to substrate ratio 500 UI/g), two strategies were evaluated for ethanol production (Simultaneous Saccharification and Fermentation, SSF and Presaccharification and Simultaneous Saccharification and Fermentation, PSSF), using an industrial and robust Saccharomyces cerevisiae strain. High concentrations of ethanol (50.2 and 48.8 g/L) and productivities (0.63 and 0.55 g/L h) were obtained by SSF and PSSF, respectively. The SSF process proved to be an advantageous strategy to obtain concentrations >6% (v/v) of ethanol with elevated conversion (95%) even employing high solid loading and non-detoxified hydrolysate. Following an integrated optimization process, cost-effective bioethanol production conditions from whole-slurry E. globulus wood were determined and validated experimentally, representing a step-forward towards its industrial implementation. |
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Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadingsEucalyptus globulus woodAutohydrolysis pretreatmentWhole-slurryHigh-solid loadingsBioethanolScience & TechnologyOne of the most important targets and challenges in the second generation bioethanol is the development of a cost-effective process on large-scale. In this context, the high solid loading on saccharification and fermentation and the use of whole-slurry from pretreatment could be promising alternatives to obtain high ethanol concentrations and to decrease operational costs and wastewater. In this work, Eucalyptus globulus wood was submitted to non-isothermal autohydrolysis treatment (Tmax = 210 °C) and the whole-slurry obtained was assayed for the optimization of enzymatic saccharification at different solid and enzymes (CTec2 and HTec2) loadings using a Box–Behnken experimental design. Under the optimized conditions (liquid solid ratio 6.4 g/g, cellulase to substrate ratio 22.5 FPU/g and hemicellulase to substrate ratio 500 UI/g), two strategies were evaluated for ethanol production (Simultaneous Saccharification and Fermentation, SSF and Presaccharification and Simultaneous Saccharification and Fermentation, PSSF), using an industrial and robust Saccharomyces cerevisiae strain. High concentrations of ethanol (50.2 and 48.8 g/L) and productivities (0.63 and 0.55 g/L h) were obtained by SSF and PSSF, respectively. The SSF process proved to be an advantageous strategy to obtain concentrations >6% (v/v) of ethanol with elevated conversion (95%) even employing high solid loading and non-detoxified hydrolysate. Following an integrated optimization process, cost-effective bioethanol production conditions from whole-slurry E. globulus wood were determined and validated experimentally, representing a step-forward towards its industrial implementation.The authors A. Roman and F.B. Pereira thank to the "Fundacao para a Ciencia e a Techologia" (FCT, Portugal) for their fellowships (grant number, SFRH/BPD/77995/2011 and SFRH/BD/64776/2009, respectively) and Gil Garrote (University of Vigo, Spain) for assistance in the pre-treatment of EGW. Research described in this article was financially supported by FEDER and FCT: Strategic Project PEst-OE/EQB/LA0023/2013, Project "BioInd-Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028'' Co-funded by the Programa Operacional Regional do Norte (ON. 2 - O Novo Norte) QREN, FEDER.Elsevier B.V.Universidade do MinhoRomaní, AloiaRuíz, Héctor A.Pereira, Francisco B.Teixeira, J. A.Domingues, Lucília20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31860engRomaní, Aloia; Ruíz, H. A.; Pereira, F. B.; Teixeira, José A.; Domingues, Lucília, Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings. Fuel, 135, 482-491, 20140016-236110.1016/j.fuel.2014.06.061info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:02Zoai:repositorium.sdum.uminho.pt:1822/31860Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:46.112284Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
title |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
spellingShingle |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings Romaní, Aloia Eucalyptus globulus wood Autohydrolysis pretreatment Whole-slurry High-solid loadings Bioethanol Science & Technology |
title_short |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
title_full |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
title_fullStr |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
title_full_unstemmed |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
title_sort |
Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings |
author |
Romaní, Aloia |
author_facet |
Romaní, Aloia Ruíz, Héctor A. Pereira, Francisco B. Teixeira, J. A. Domingues, Lucília |
author_role |
author |
author2 |
Ruíz, Héctor A. Pereira, Francisco B. Teixeira, J. A. Domingues, Lucília |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Romaní, Aloia Ruíz, Héctor A. Pereira, Francisco B. Teixeira, J. A. Domingues, Lucília |
dc.subject.por.fl_str_mv |
Eucalyptus globulus wood Autohydrolysis pretreatment Whole-slurry High-solid loadings Bioethanol Science & Technology |
topic |
Eucalyptus globulus wood Autohydrolysis pretreatment Whole-slurry High-solid loadings Bioethanol Science & Technology |
description |
One of the most important targets and challenges in the second generation bioethanol is the development of a cost-effective process on large-scale. In this context, the high solid loading on saccharification and fermentation and the use of whole-slurry from pretreatment could be promising alternatives to obtain high ethanol concentrations and to decrease operational costs and wastewater. In this work, Eucalyptus globulus wood was submitted to non-isothermal autohydrolysis treatment (Tmax = 210 °C) and the whole-slurry obtained was assayed for the optimization of enzymatic saccharification at different solid and enzymes (CTec2 and HTec2) loadings using a Box–Behnken experimental design. Under the optimized conditions (liquid solid ratio 6.4 g/g, cellulase to substrate ratio 22.5 FPU/g and hemicellulase to substrate ratio 500 UI/g), two strategies were evaluated for ethanol production (Simultaneous Saccharification and Fermentation, SSF and Presaccharification and Simultaneous Saccharification and Fermentation, PSSF), using an industrial and robust Saccharomyces cerevisiae strain. High concentrations of ethanol (50.2 and 48.8 g/L) and productivities (0.63 and 0.55 g/L h) were obtained by SSF and PSSF, respectively. The SSF process proved to be an advantageous strategy to obtain concentrations >6% (v/v) of ethanol with elevated conversion (95%) even employing high solid loading and non-detoxified hydrolysate. Following an integrated optimization process, cost-effective bioethanol production conditions from whole-slurry E. globulus wood were determined and validated experimentally, representing a step-forward towards its industrial implementation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31860 |
url |
http://hdl.handle.net/1822/31860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Romaní, Aloia; Ruíz, H. A.; Pereira, F. B.; Teixeira, José A.; Domingues, Lucília, Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings. Fuel, 135, 482-491, 2014 0016-2361 10.1016/j.fuel.2014.06.061 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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