Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese

Detalhes bibliográficos
Autor(a) principal: Decontardi, Simone
Data de Publicação: 2018
Outros Autores: Soares, Célia Maria Gonçalves, Lima, Nelson, Battilani, Paola
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/51455
Resumo: "Available online 18 January 2018"
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spelling Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheeseAspergillusPenicilliumMycobiotaMycotoxinBioloadScience & Technology"Available online 18 January 2018"Penicillium and Aspergillus genera, both including mycotoxin producing species, were reported as associated to cheese and cheese working environment, but never studied in an extensive way in Italian grana cheese (Grana Padano and Parmigiano Reggiano). The aim of this work was to address the identification of Aspergilli and Penicillia associated to grana cheese in order to lay down the basis for risk assessment and safe processing for a high quality production. One hundred and four strains belonging to Aspergillus and Penicillium genera were obtained from cheese crust and from ripening room air (with the latter largely dominant), and identified following a polyphasic approach, strongly required for the identification at the species level. Morphological observation was used along with molecular techniques, RAPD-PCR fingerprinting and calmodulin gene sequencing (CaM), the former aimed to limit as much as possible the latter sequencing effort. Seventy four percent of the strains were assigned to Penicillium subgenus Penicillium, section Fasciculata. Main mycotoxin producing species identified were A. flavus, P. crustosum and P. verrucosum, while the dominant species in both air and cheese crust was P. solitum, which has never been so far reported as mycotoxigenic. Results obtained in this study confirmed that mycotoxin contamination is a possible issue to face during grana cheese making.The present work was supported by the following institutions: Consorzio del Formaggio Parmigiano Reggiano, Consorzio per la tutela del Formaggio Grana Padano, Fondazione Romeo ed Enrica Invernizzi, the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/ 2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Simone Decontardi carried out this work within the PhD school “Agrisystem” of the Universita Cattolica del Sacro Cuore (Italy).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoDecontardi, SimoneSoares, Célia Maria GonçalvesLima, NelsonBattilani, Paola2018-082018-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51455engDecontardi, Simone; Soares, Célia; Lima, Nelson; Battilani, Paola, Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese.0740-00201095-999810.1016/j.fm.2018.01.01229526199http://www.elsevier.com/locate/issn/07400020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:22Zoai:repositorium.sdum.uminho.pt:1822/51455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:28.671895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
title Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
spellingShingle Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
Decontardi, Simone
Aspergillus
Penicillium
Mycobiota
Mycotoxin
Bioload
Science & Technology
title_short Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
title_full Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
title_fullStr Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
title_full_unstemmed Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
title_sort Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
author Decontardi, Simone
author_facet Decontardi, Simone
Soares, Célia Maria Gonçalves
Lima, Nelson
Battilani, Paola
author_role author
author2 Soares, Célia Maria Gonçalves
Lima, Nelson
Battilani, Paola
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Decontardi, Simone
Soares, Célia Maria Gonçalves
Lima, Nelson
Battilani, Paola
dc.subject.por.fl_str_mv Aspergillus
Penicillium
Mycobiota
Mycotoxin
Bioload
Science & Technology
topic Aspergillus
Penicillium
Mycobiota
Mycotoxin
Bioload
Science & Technology
description "Available online 18 January 2018"
publishDate 2018
dc.date.none.fl_str_mv 2018-08
2018-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/51455
url http://hdl.handle.net/1822/51455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Decontardi, Simone; Soares, Célia; Lima, Nelson; Battilani, Paola, Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese.
0740-0020
1095-9998
10.1016/j.fm.2018.01.012
29526199
http://www.elsevier.com/locate/issn/07400020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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