Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/51455 |
Resumo: | "Available online 18 January 2018" |
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Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheeseAspergillusPenicilliumMycobiotaMycotoxinBioloadScience & Technology"Available online 18 January 2018"Penicillium and Aspergillus genera, both including mycotoxin producing species, were reported as associated to cheese and cheese working environment, but never studied in an extensive way in Italian grana cheese (Grana Padano and Parmigiano Reggiano). The aim of this work was to address the identification of Aspergilli and Penicillia associated to grana cheese in order to lay down the basis for risk assessment and safe processing for a high quality production. One hundred and four strains belonging to Aspergillus and Penicillium genera were obtained from cheese crust and from ripening room air (with the latter largely dominant), and identified following a polyphasic approach, strongly required for the identification at the species level. Morphological observation was used along with molecular techniques, RAPD-PCR fingerprinting and calmodulin gene sequencing (CaM), the former aimed to limit as much as possible the latter sequencing effort. Seventy four percent of the strains were assigned to Penicillium subgenus Penicillium, section Fasciculata. Main mycotoxin producing species identified were A. flavus, P. crustosum and P. verrucosum, while the dominant species in both air and cheese crust was P. solitum, which has never been so far reported as mycotoxigenic. Results obtained in this study confirmed that mycotoxin contamination is a possible issue to face during grana cheese making.The present work was supported by the following institutions: Consorzio del Formaggio Parmigiano Reggiano, Consorzio per la tutela del Formaggio Grana Padano, Fondazione Romeo ed Enrica Invernizzi, the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/ 2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Simone Decontardi carried out this work within the PhD school “Agrisystem” of the Universita Cattolica del Sacro Cuore (Italy).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoDecontardi, SimoneSoares, Célia Maria GonçalvesLima, NelsonBattilani, Paola2018-082018-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51455engDecontardi, Simone; Soares, Célia; Lima, Nelson; Battilani, Paola, Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese.0740-00201095-999810.1016/j.fm.2018.01.01229526199http://www.elsevier.com/locate/issn/07400020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:22Zoai:repositorium.sdum.uminho.pt:1822/51455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:28.671895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
title |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
spellingShingle |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese Decontardi, Simone Aspergillus Penicillium Mycobiota Mycotoxin Bioload Science & Technology |
title_short |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
title_full |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
title_fullStr |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
title_full_unstemmed |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
title_sort |
Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese |
author |
Decontardi, Simone |
author_facet |
Decontardi, Simone Soares, Célia Maria Gonçalves Lima, Nelson Battilani, Paola |
author_role |
author |
author2 |
Soares, Célia Maria Gonçalves Lima, Nelson Battilani, Paola |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Decontardi, Simone Soares, Célia Maria Gonçalves Lima, Nelson Battilani, Paola |
dc.subject.por.fl_str_mv |
Aspergillus Penicillium Mycobiota Mycotoxin Bioload Science & Technology |
topic |
Aspergillus Penicillium Mycobiota Mycotoxin Bioload Science & Technology |
description |
"Available online 18 January 2018" |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08 2018-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51455 |
url |
http://hdl.handle.net/1822/51455 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Decontardi, Simone; Soares, Célia; Lima, Nelson; Battilani, Paola, Polyphasic identification of Penicillia and Aspergilli isolated from Italian grana cheese. 0740-0020 1095-9998 10.1016/j.fm.2018.01.012 29526199 http://www.elsevier.com/locate/issn/07400020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133019839135744 |