Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS

Detalhes bibliográficos
Autor(a) principal: Ložnjak Švarc, Petra
Data de Publicação: 2020
Outros Autores: Oveland, E., Strandler, H.S., Kariluoto, S., Campos-Giménez, E., Ivarsen, E., Malaviole, I., Motta, Carla, Rychlik, M., Striegel, L., Jakobsen, J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7276
Resumo: Many thanks to Dr. Andrew Hanson (University of Florida) and Dr. Rocío Díaz de la Garza (Technologíco de Monterrey) who generously gave us the permission to produce the GGH used in this study, as well as permission to establish a commercial production in the future. Authors are very grateful to Merck and Phenomenex for providing a significant discount for the internal standards and the SPE columns used, and to NMKL to be responsible for shipping cost. We would like to thank the following individuals for their contribution with the analyses: Nina Wollertsen, Tone Myhra, Susanna Petrova, and Torill Berg at IMR (Bergen, Norway); Sylvie Bénet at Nestlé Research (Lausanne, Switzerland); Brian Hermansen at Eurofins Vitamin Testing (Vejen, Denmark); Marlène Daminato at Laboratoire Aquanal (Pessac, France); Tiago Moreira at INSA (Lisbon, Portugal) and to Heidi Jahn at DTU Food (Denmark) for assisting with standards and packing.
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spelling Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MSTetrahydrofolatesInter-laboratory StudyPlant EnzymeDeconjugaseHomogenizationComposição dos AlimentosMany thanks to Dr. Andrew Hanson (University of Florida) and Dr. Rocío Díaz de la Garza (Technologíco de Monterrey) who generously gave us the permission to produce the GGH used in this study, as well as permission to establish a commercial production in the future. Authors are very grateful to Merck and Phenomenex for providing a significant discount for the internal standards and the SPE columns used, and to NMKL to be responsible for shipping cost. We would like to thank the following individuals for their contribution with the analyses: Nina Wollertsen, Tone Myhra, Susanna Petrova, and Torill Berg at IMR (Bergen, Norway); Sylvie Bénet at Nestlé Research (Lausanne, Switzerland); Brian Hermansen at Eurofins Vitamin Testing (Vejen, Denmark); Marlène Daminato at Laboratoire Aquanal (Pessac, France); Tiago Moreira at INSA (Lisbon, Portugal) and to Heidi Jahn at DTU Food (Denmark) for assisting with standards and packing.Quantification of the specific folate vitamers to estimate total folate in foods is not standardized. A collaborative study, including eight European laboratories, was conducted in order to determine the repeatability and reproducibility of the method for folate quantification in foods using the plant-origin γ-glutamyl hydrolase as part of the extraction procedure. The seven food samples analyzed represent the food groups; fruits, vegetables, dairy products, legumes, offal, fish, and fortified infant formula. The homogenization step was included, and six folate vitamers were analyzed using LC-MS/MS. Total folate content, expressed as folic acid equivalent, was 17–490 μg/100 g in all samples. Horwitz ratio values were within the acceptable range (0.60–1.94), except for fish. The results for fortified infant formula, a certified reference material (NIST 1869), confirmed the trueness of the method. The collaborative study is part of a standardization project within the Nordic Committee on Food Analysis (NMKL).Highlights: Specific LC-MS/MS method for folate analysis in food is inter-laboratory validated; Total folate results acceptable in six tested food samples; for salmon unacceptable; HorRat values for total folate and 5-CH3-H4folate were within the acceptable range; Acceptability of HorRat values for other studied vitamers depends on food matrix; For total folate: repeatability at 2.3%–15% and reproducibility at 8.7%–58%ElsevierRepositório Científico do Instituto Nacional de SaúdeLožnjak Švarc, PetraOveland, E.Strandler, H.S.Kariluoto, S.Campos-Giménez, E.Ivarsen, E.Malaviole, I.Motta, CarlaRychlik, M.Striegel, L.Jakobsen, J.2022-07-04T00:30:21Z2020-12-152020-12-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7276engFood Chem. 2020 Dec 15;333:127447. doi:10.1016/j.foodchem.2020.127447. Epub 2020 Jul 4.0308-8146https://doi.org/10.1016/j.foodchem.2020.127447.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:03Zoai:repositorio.insa.pt:10400.18/7276Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:10.086707Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
title Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
spellingShingle Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
Ložnjak Švarc, Petra
Tetrahydrofolates
Inter-laboratory Study
Plant Enzyme
Deconjugase
Homogenization
Composição dos Alimentos
title_short Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
title_full Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
title_fullStr Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
title_full_unstemmed Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
title_sort Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
author Ložnjak Švarc, Petra
author_facet Ložnjak Švarc, Petra
Oveland, E.
Strandler, H.S.
Kariluoto, S.
Campos-Giménez, E.
Ivarsen, E.
Malaviole, I.
Motta, Carla
Rychlik, M.
Striegel, L.
Jakobsen, J.
author_role author
author2 Oveland, E.
Strandler, H.S.
Kariluoto, S.
Campos-Giménez, E.
Ivarsen, E.
Malaviole, I.
Motta, Carla
Rychlik, M.
Striegel, L.
Jakobsen, J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Ložnjak Švarc, Petra
Oveland, E.
Strandler, H.S.
Kariluoto, S.
Campos-Giménez, E.
Ivarsen, E.
Malaviole, I.
Motta, Carla
Rychlik, M.
Striegel, L.
Jakobsen, J.
dc.subject.por.fl_str_mv Tetrahydrofolates
Inter-laboratory Study
Plant Enzyme
Deconjugase
Homogenization
Composição dos Alimentos
topic Tetrahydrofolates
Inter-laboratory Study
Plant Enzyme
Deconjugase
Homogenization
Composição dos Alimentos
description Many thanks to Dr. Andrew Hanson (University of Florida) and Dr. Rocío Díaz de la Garza (Technologíco de Monterrey) who generously gave us the permission to produce the GGH used in this study, as well as permission to establish a commercial production in the future. Authors are very grateful to Merck and Phenomenex for providing a significant discount for the internal standards and the SPE columns used, and to NMKL to be responsible for shipping cost. We would like to thank the following individuals for their contribution with the analyses: Nina Wollertsen, Tone Myhra, Susanna Petrova, and Torill Berg at IMR (Bergen, Norway); Sylvie Bénet at Nestlé Research (Lausanne, Switzerland); Brian Hermansen at Eurofins Vitamin Testing (Vejen, Denmark); Marlène Daminato at Laboratoire Aquanal (Pessac, France); Tiago Moreira at INSA (Lisbon, Portugal) and to Heidi Jahn at DTU Food (Denmark) for assisting with standards and packing.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-15
2020-12-15T00:00:00Z
2022-07-04T00:30:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7276
url http://hdl.handle.net/10400.18/7276
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Chem. 2020 Dec 15;333:127447. doi:10.1016/j.foodchem.2020.127447. Epub 2020 Jul 4.
0308-8146
https://doi.org/10.1016/j.foodchem.2020.127447.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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