Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi

Detalhes bibliográficos
Autor(a) principal: Leitão, Ana Lúcia
Data de Publicação: 2021
Outros Autores: Enguita, Francisco J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/50058
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungiBiodegradationMycotoxinOchratoxin AOchratoxinaseStructural biology© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Background: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and characterization of low-frequency protein motions, we performed a proteome mining in filamentous fungi to characterize new enzymes with potential ochratoxinase activity. Results: the proteome mining results demonstrated the ubiquitous presence of fungal binuclear zinc-dependent amido-hydrolases with a high degree of structural homology to the already characterized ochratoxinase from Aspergillus niger. Ochratoxinase-like enzymes from ochratoxin-producing fungi showed more favorable substrate-binding pockets to accommodate ochratoxins A and B. Conclusions: filamentous fungi are an interesting and rich source of hydrolases potentially capable of degrading ochratoxins, and could be used for the detoxification of diverse food commodities.This work is supported by the Portuguese Foundation for Science and Technology (FCT) under the framework of the research grant PTDC-MED-GEN-29389-2017.MDPIRepositório da Universidade de LisboaLeitão, Ana LúciaEnguita, Francisco J.2021-10-29T14:12:41Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50058engBiomolecules. 2021 Jul 16;11(7):104010.3390/biom110710402218-273Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:06Zoai:repositorio.ul.pt:10451/50058Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:34.711005Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
title Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
spellingShingle Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
Leitão, Ana Lúcia
Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
title_short Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
title_full Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
title_fullStr Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
title_full_unstemmed Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
title_sort Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
author Leitão, Ana Lúcia
author_facet Leitão, Ana Lúcia
Enguita, Francisco J.
author_role author
author2 Enguita, Francisco J.
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Leitão, Ana Lúcia
Enguita, Francisco J.
dc.subject.por.fl_str_mv Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
topic Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021-10-29T14:12:41Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/50058
url http://hdl.handle.net/10451/50058
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomolecules. 2021 Jul 16;11(7):1040
10.3390/biom11071040
2218-273X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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