Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/50058 |
Resumo: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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7160 |
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Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungiBiodegradationMycotoxinOchratoxin AOchratoxinaseStructural biology© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Background: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and characterization of low-frequency protein motions, we performed a proteome mining in filamentous fungi to characterize new enzymes with potential ochratoxinase activity. Results: the proteome mining results demonstrated the ubiquitous presence of fungal binuclear zinc-dependent amido-hydrolases with a high degree of structural homology to the already characterized ochratoxinase from Aspergillus niger. Ochratoxinase-like enzymes from ochratoxin-producing fungi showed more favorable substrate-binding pockets to accommodate ochratoxins A and B. Conclusions: filamentous fungi are an interesting and rich source of hydrolases potentially capable of degrading ochratoxins, and could be used for the detoxification of diverse food commodities.This work is supported by the Portuguese Foundation for Science and Technology (FCT) under the framework of the research grant PTDC-MED-GEN-29389-2017.MDPIRepositório da Universidade de LisboaLeitão, Ana LúciaEnguita, Francisco J.2021-10-29T14:12:41Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50058engBiomolecules. 2021 Jul 16;11(7):104010.3390/biom110710402218-273Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:06Zoai:repositorio.ul.pt:10451/50058Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:34.711005Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
title |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
spellingShingle |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi Leitão, Ana Lúcia Biodegradation Mycotoxin Ochratoxin A Ochratoxinase Structural biology |
title_short |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
title_full |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
title_fullStr |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
title_full_unstemmed |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
title_sort |
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi |
author |
Leitão, Ana Lúcia |
author_facet |
Leitão, Ana Lúcia Enguita, Francisco J. |
author_role |
author |
author2 |
Enguita, Francisco J. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Leitão, Ana Lúcia Enguita, Francisco J. |
dc.subject.por.fl_str_mv |
Biodegradation Mycotoxin Ochratoxin A Ochratoxinase Structural biology |
topic |
Biodegradation Mycotoxin Ochratoxin A Ochratoxinase Structural biology |
description |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-29T14:12:41Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/50058 |
url |
http://hdl.handle.net/10451/50058 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomolecules. 2021 Jul 16;11(7):1040 10.3390/biom11071040 2218-273X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134564567744512 |