Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Detalhes bibliográficos
Autor(a) principal: Guerreiro, Adriana
Data de Publicação: 2020
Outros Autores: Andrade, Mariana, Menezes, Carina, Vilarinho, Fernanda, Dias, Elsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7501
Resumo: The HEK293T cell line and reagents for cell culture were kindly offered by Rafaela Lacerda, Department of Human Genetics, National Health Institute, Lisbon, Portugal.
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spelling Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological ApplicationsCyanobacteriaAntioxidant PotentialNatural AntioxidantsDPPH Assayβ-carotene Bleaching AssayPhenolicFlavonoidsHEK293T Cell LineCytotoxicity PreventionSegurança AlimentarComposição dos AlimentosToxicologia dos AlimentosToxicologiaAgentes Microbianos e AmbienteThe HEK293T cell line and reagents for cell culture were kindly offered by Rafaela Lacerda, Department of Human Genetics, National Health Institute, Lisbon, Portugal.Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (H2O2-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE µg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a “per cell” or “per cell volume” base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the H2O2-induced cytotoxicity. Freshwater Aphanizomenon gracile (LMECYA 009) and Aphanizomenon flos-aquae (LMECYA 088), terrestrial Nostoc (LMECYA 291) and wastewater Planktothrix mougeotii (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.MDPIRepositório Científico do Instituto Nacional de SaúdeGuerreiro, AdrianaAndrade, MarianaMenezes, CarinaVilarinho, FernandaDias, Elsa2021-03-15T16:53:35Z2020-08-262020-08-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7501engToxins (Basel). 2020 Aug 26;12(9):548. doi: 10.3390/toxins120905482072-665110.3390/toxins12090548info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:05Zoai:repositorio.insa.pt:10400.18/7501Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:12.415053Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
title Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
spellingShingle Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
Guerreiro, Adriana
Cyanobacteria
Antioxidant Potential
Natural Antioxidants
DPPH Assay
β-carotene Bleaching Assay
Phenolic
Flavonoids
HEK293T Cell Line
Cytotoxicity Prevention
Segurança Alimentar
Composição dos Alimentos
Toxicologia dos Alimentos
Toxicologia
Agentes Microbianos e Ambiente
title_short Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
title_full Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
title_fullStr Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
title_full_unstemmed Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
title_sort Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications
author Guerreiro, Adriana
author_facet Guerreiro, Adriana
Andrade, Mariana
Menezes, Carina
Vilarinho, Fernanda
Dias, Elsa
author_role author
author2 Andrade, Mariana
Menezes, Carina
Vilarinho, Fernanda
Dias, Elsa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Guerreiro, Adriana
Andrade, Mariana
Menezes, Carina
Vilarinho, Fernanda
Dias, Elsa
dc.subject.por.fl_str_mv Cyanobacteria
Antioxidant Potential
Natural Antioxidants
DPPH Assay
β-carotene Bleaching Assay
Phenolic
Flavonoids
HEK293T Cell Line
Cytotoxicity Prevention
Segurança Alimentar
Composição dos Alimentos
Toxicologia dos Alimentos
Toxicologia
Agentes Microbianos e Ambiente
topic Cyanobacteria
Antioxidant Potential
Natural Antioxidants
DPPH Assay
β-carotene Bleaching Assay
Phenolic
Flavonoids
HEK293T Cell Line
Cytotoxicity Prevention
Segurança Alimentar
Composição dos Alimentos
Toxicologia dos Alimentos
Toxicologia
Agentes Microbianos e Ambiente
description The HEK293T cell line and reagents for cell culture were kindly offered by Rafaela Lacerda, Department of Human Genetics, National Health Institute, Lisbon, Portugal.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-26
2020-08-26T00:00:00Z
2021-03-15T16:53:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7501
url http://hdl.handle.net/10400.18/7501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxins (Basel). 2020 Aug 26;12(9):548. doi: 10.3390/toxins12090548
2072-6651
10.3390/toxins12090548
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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