dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/684 |
Resumo: | Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. |
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dachshund Potentiates Hedgehog Signaling during Drosophila RetinogenesisHedgehog signalingCloningEyesCell differentiationCell cycle and cell divisionImaginal discsDrosophila melanogasterNeuronal differentiationProper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis.MINECO Spain grants: (BFU2012-34324, BFU2015- 66040); Research Foundation—Flanders FWO grants: (G.0640.13, G.0791.14, PhD fellowship); Fundação para a Ciência e Tecnologia grant: (IF/01031/2012).Public Library of ScienceARCABrás-Pereira, CatarinaPotier, DelphineJacobs, JelleAerts, SteinCasares, FernandoJanody, Florence2016-08-11T15:31:33Z2016-07-212016-07-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/684engBrás-Pereira C, Potier D, Jacobs J, Aerts S, Casares F, Janody F (2016) dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genet 12(7): e1006204. doi:10.1371/journal.pgen.100620410.1371/journal.pgen.1006204info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:04Zoai:arca.igc.gulbenkian.pt:10400.7/684Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:55.165836Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
spellingShingle |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis Brás-Pereira, Catarina Hedgehog signaling Cloning Eyes Cell differentiation Cell cycle and cell division Imaginal discs Drosophila melanogaster Neuronal differentiation |
title_short |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_full |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_fullStr |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_full_unstemmed |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_sort |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
author |
Brás-Pereira, Catarina |
author_facet |
Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence |
author_role |
author |
author2 |
Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence |
dc.subject.por.fl_str_mv |
Hedgehog signaling Cloning Eyes Cell differentiation Cell cycle and cell division Imaginal discs Drosophila melanogaster Neuronal differentiation |
topic |
Hedgehog signaling Cloning Eyes Cell differentiation Cell cycle and cell division Imaginal discs Drosophila melanogaster Neuronal differentiation |
description |
Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-11T15:31:33Z 2016-07-21 2016-07-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/684 |
url |
http://hdl.handle.net/10400.7/684 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brás-Pereira C, Potier D, Jacobs J, Aerts S, Casares F, Janody F (2016) dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genet 12(7): e1006204. doi:10.1371/journal.pgen.1006204 10.1371/journal.pgen.1006204 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130574673149952 |