Notch signaling in mouse blastocyst development and hatching

Detalhes bibliográficos
Autor(a) principal: Batista, Mariana R.
Data de Publicação: 2020
Outros Autores: Diniz, Patrícia, Torres, Ana, de Moura Murta, Daniel, Lopes-da-Costa, Luís, Silva, Elisabete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24102
Resumo: Research Areas: Developmental Biology
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spelling Notch signaling in mouse blastocyst development and hatchingBlastocystDevelopmentHatchingNotchMouseResearch Areas: Developmental BiologyBackground: Mammalian early embryo development requires a well-orchestrated interplay of cell signaling pathways. Notch is a major regulatory pathway involved in cell-fate determination in embryonic and adult scenarios. However, the role of Notch in embryonic pre-implantation development is controversial. In particular, Notch role on blastocyst development and hatching remains elusive, and a complete picture of the transcription and expression patterns of Notch components during this time-period is not available. Results: This study provided a comprehensive view on the dynamics of individual embryo gene transcription and protein expression patterns of Notch components (receptors Notch1–4; ligands Dll1 and Dll4, Jagged1–2; and effectors Hes1–2), and their relationship with transcription of gene markers of pluripotency and differentiation (Sox2, Oct4, Klf4, Cdx2) during mouse blastocyst development and hatching. Transcription of Notch1–2, Jagged1–2 and Hes1 was highly prevalent and dynamic along stages of development, whereas transcription of Notch3–4, Dll4 and Hes2 had a low prevalence among embryos. Transcription levels of Notch1, Notch2, Jagged2 and Hes1 correlated with each other and with those of pluripotency and differentiation genes. Gene transcription was associated to protein expression, except for Jagged2, where high transcription levels in all embryos were not translated into protein. Presence of Notch signaling activity was confirmed through nuclear NICD and Hes1 detection, and downregulation of Hes1 transcription following canonical signaling blockade with DAPT. In vitro embryo culture supplementation with Jagged1 had no effect on embryo developmental kinetics. In contrast, supplementation with Jagged2 abolished Jagged1 transcription, downregulated Cdx2 transcription and inhibited blastocyst hatching. Notch signaling blockade by DAPT downregulated transcription of Sox2, and retarded embryo hatching. Conclusion: Transcription of Notch genes showed a dynamic pattern along blastocyst development and hatching. Data confirmed Notch signaling activity, and lead to the suggestion that Notch canonical signaling may be operating through Notch1, Notch3, Jagged1 and Hes1. Embryo culture supplementation with Jagged1 and Jagged2 unveiled a possible regulatory effect between Jagged1, Cdx2 and blastocyst hatching. Overall, results indicate that a deregulation in Notch signaling, either by its over or under-activation, affects blastocyst development and hatching.BMCRepositório da Universidade de LisboaBatista, Mariana R.Diniz, PatríciaTorres, Anade Moura Murta, DanielLopes-da-Costa, LuísSilva, Elisabete2022-04-18T16:51:54Z2020-06-022020-06-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24102engBatista MR, Diniz P, Torres A, Murta D, Lopes-da-Costa L, Silva E. 2020. Notch signaling in mouse blastocyst development and hatching, BMC Developmental Biology, 20(1):9. Doi 10.1186/s12861-020-00216-21471-213X10.1186/s12861-020-00216-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24102Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.415025Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Notch signaling in mouse blastocyst development and hatching
title Notch signaling in mouse blastocyst development and hatching
spellingShingle Notch signaling in mouse blastocyst development and hatching
Batista, Mariana R.
Blastocyst
Development
Hatching
Notch
Mouse
title_short Notch signaling in mouse blastocyst development and hatching
title_full Notch signaling in mouse blastocyst development and hatching
title_fullStr Notch signaling in mouse blastocyst development and hatching
title_full_unstemmed Notch signaling in mouse blastocyst development and hatching
title_sort Notch signaling in mouse blastocyst development and hatching
author Batista, Mariana R.
author_facet Batista, Mariana R.
Diniz, Patrícia
Torres, Ana
de Moura Murta, Daniel
Lopes-da-Costa, Luís
Silva, Elisabete
author_role author
author2 Diniz, Patrícia
Torres, Ana
de Moura Murta, Daniel
Lopes-da-Costa, Luís
Silva, Elisabete
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Batista, Mariana R.
Diniz, Patrícia
Torres, Ana
de Moura Murta, Daniel
Lopes-da-Costa, Luís
Silva, Elisabete
dc.subject.por.fl_str_mv Blastocyst
Development
Hatching
Notch
Mouse
topic Blastocyst
Development
Hatching
Notch
Mouse
description Research Areas: Developmental Biology
publishDate 2020
dc.date.none.fl_str_mv 2020-06-02
2020-06-02T00:00:00Z
2022-04-18T16:51:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24102
url http://hdl.handle.net/10400.5/24102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Batista MR, Diniz P, Torres A, Murta D, Lopes-da-Costa L, Silva E. 2020. Notch signaling in mouse blastocyst development and hatching, BMC Developmental Biology, 20(1):9. Doi 10.1186/s12861-020-00216-2
1471-213X
10.1186/s12861-020-00216-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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